Size Exclusion Chromatography | Lab Procedure

#BaaYo
Gel filtration chromatography (also known as size exclusion chromatography, molecular sieve chromatography, or gel permeation chromatography) is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight.
In gel filtration chromatography, the stationary phase is comprised of porous beads packed into a column. The mobile phase is the running buffer or other solvent. Sample components partition between the stationary and mobile phases based on their size-based accessibility to the pores of the matrix beads.
The smaller the size of the molecule or particle, the greater access it has to the pores of the matrix (or the accessible stationary phase), and the slower it moves through the column. Very large particles, which are completely excluded from the pores, elute first from the column at the void volume.
The separated components are visualized as a plot of the volume of the mobile phase eluted through the column versus the detector signal. This plot, called a chromatogram, shows the location of the individual peaks and the quality of resolution of these peaks.The location of the peaks, their heights, and widths convey a great deal of information about the sample components as well as the column.
In this video we have discussed complete laboratory procedure of size exclusion chromatography and also, how to calculate KD (distribution coefficient) of a particular component from chromatogram (Graph) and how KD valve can be use to determine the molecular weights of the sample components.
Please watch this video for more details.
Please SHARE with all your friends , SUPPORT and SUBSCRIBE to this channel !
Subscribe: www.youtube.co....
Related Videos:
Column Chromatography: • Column Chromatography ...
HPLC Chromatography: • HPLC Chromatography Ba...
Thin Layer Chromatography : • Thin Layer Chromatogra...
Ion Exchange Chromatography : • Ion Exchange Chromatog...
Gas Chromatography: • Gas Chromatography Pri...
Subscribe: www.youtube.co....

[@BaaYo]

To access notes and practice questions, please join our Telegram channel t.me/baayo_official

[@Al-Imtiaz]

You deserve millions of likes.....best conceptual teacher ever. Much love and respect from Pakistan😍😍😍😍😍😍😍

[@cookiebank6909]

Mam u are really great ,i understand all chromatography techniques one day before exam . Amazing love from Pakistan today ❤❤❤

[@sidraghu1c]

Mam your way of teaching is marvelous. You are really helping alot to make our concept crisp clear Waiting for more videos Thanks alot mam

[@razorr1920]

Just wanted to say thanks and congratulate you on the good work you have put through. I am a Flight engineer by profession, but love to learn. I found your presentation of the topic very elaborate and accurate. Will be following you...😊

[@BaaYo]

Thanks a lot 😊, your feedback is really motivating 😊🙏🏻

[@anchalkoundal745]

Mam thank you so much for your all videos on types of chromatography. Because of these videos I am able to learn chromatography and their types very easily ...thank you for your help 😊😊 .

[@BaaYo]

Thank You very much Anchal 😊😊, really like you comment.

[@hayaahmad8133]

Whole topic was explained well! Love from PAKISTAN... KEEP IT UP 👍

[@marva7991]

Thank you so much such a brief yet descriptive video. Loved learning about it. I used to find this topic boring but not now :)

[@shanu9094]

Fantastic mam I saw your all vedios based upon chromatography and I just loved the way u described every single point of topic very clearly 😊😊😊😊

[@BaaYo]

Thank You Shanu 😊, I am glad that my videos could help you with these topics

[@shanu9094]

@@BaaYo mam I don't like those vedio which based on electrophoresis on you tube plzzz upload your vedios on the topic electrophoresis

[@BaaYo]

its live now : kzbin.info/www/bejne/mZrXfqlmnduZr8U hope it will met with your expectation

[@siraj2343]

we need a lecture on Sedimentation basic principles of centrifugation.. and maam ur videos are so good and helpful for us .

[@VijayPatel-cr8pq]

Very nice maam...🙏

[@devaguptapuchayasri8842]

Ma'am thank you very much for the brief explanation... I'm now able to learn different Chromatographic techniques easily by watching your videos..

[@navnathdhage632]

mam ur teaching is very clear and very nice speaking and also they are very helpfull

[@BaaYo]

Thank You Navath 🙏🏻😊

[@saurabhshrivastva4641]

ma'am Bahut achha samjhati hain ap.........Dhanyavad👌👌

[@mdaabidhussain7039]

Ma'am aapka smjhane ka tareeka bhut accha hai.. Thank you ma'am 😊♥️

[@Krishnakrutka]

Thank you so much ma'am for making the concept clear. I was tensed about chromatography topic and now you made it easier for me🌷❤

[@Nuzhatsheikh16]

It's very good video, help in my studies and my dought get cleared my watching these videos

[@shubhamgade3164]

Mam your videos are very easy to understand And your explanation is very nice thank u mam

[@monica6899]

Thank you soo much mam now I understand chromatography easily thank you once again for your efforts 🖤✨ Love from Nagpur . ❤️

[@19deepikayadav14]

Very nicely explained mam Tysm sab samjha.

[@mrniravpatel9591]

I learn main basic principles about chromatography in your video I am not learn this kind of basic thing in my 2 years carrier in industries

[@BaaYo]

🙏🏻🙏🏻 thanks Nirav.

[@Ankitsingh-qf6el]

Bhai kaun si industry m kaam kr rahe the😂😂

[@jhalendraverma7415]

@@Ankitsingh-qf6el ab to 5 saal ho gya Hoga😂

[@priyankavhatkar8070]

The Way u teach is too good..Perfection💯. Thank you.

[@BaaYo]

It's my pleasure

[@ajayprakash4307]

Hii Priyanka where r u from You have and notes of MSC CHEMISTRY 4th Sem?

[@pragatimishra786]

Easily explained concept ... Thank you Ma'am.

[@radheradhe1371]

Mam iam loving ❤️❤️ your teching way

[@musicstudio554]

I Learn main basic concept about technique in your lecture mam thank u so much mam for very informative easy lecture

[@madhumitanishad9462]

Your voice and your teaching techniques is very good maim👌👌👏

[@aysha840]

Assalam o alikum Mam very impressed by your teaching method. 💓🙋

[@nasirchauhan3622]

You are a brilliant teacher Mam and cute also you are beautibrillinty teachers

[@ajimshaikh8779]

Thanks ma'am way of explaining is very good can easily understand...

[@shikamarunara2827]

Wish my teachers could teach like u

[@DpkTamta157]

Thanku mam ap bahut achha explaine krte ho.

[@ajayprakash4307]

Thankuuuú Ma'm your Explanation Technique is So Good and Incredible 👌👌😍😍

[@narendraverma1017]

Awesome explanation and nice presentation madam

[@muhammadumer4759]

Mam ,i really appreciate u for sharing such valuable information .

[@PrakashChandraJoshii]

After 3 days I'm going to attend exam, after watching all your video on chromatography , I'm sure i will pass the exam (I'm job holder no time to study) Love from Nepal ❤🇳🇵

[@harisapkotavlogs]

Ah bro Analytical chemistry xa yr 😆

[@PrakashChandraJoshii]

Center ka ho bro ? TC bata ho ?

[@harisapkotavlogs]

@@PrakashChandraJoshii haina TC ta DMC ho

[@manjulasatishmanjulasatish85]

Thank you so much mam u r the saviour!! explanation is mind blowing ❤

[@anupriyamedhi2729]

Thank you so much maam.😊

[@POOJACHAUHAN-le9sg]

Hello mam your video clear my concept and very useful for pharmacy profession .I request you to please make details video on hplc And handling of hplc and it's software please I request depth of my heart

[@shaikayeshasiddiqi3895]

Tqs to u mam to clear al doubts about these topics Happy teachers day💐mam

[@pradeepkumarsingh742]

very nice mam

[@suraj.rathod4670]

Good explanation

[@hazratullahkhan4661]

Beautiful lecture with beautiful face ❤❤

[@sunilmaahi]

every video is superb with quality... thnx

[@BaaYo]

Thank You Sunil 🙏🏻😊

[@suhalah]

Dynamic light spectroscopy would be very helpful

[@rohitdhiman1931]

Thank you so much for clear my concept

[@nazianoreen9192]

❤❤❤❤outstanding teacher

[@ayeshajabeen2654]

Superb explanation mam.

[@niharikakashyap5649]

Thank you so much mem this video has helped me a lot 🙏🙏🙏

[@AmandeepKaur-cy8ds]

It was well explained in a very simple manner 👍👍

[@chinmayeethakur5223]

So clear and concise ♥️

[@akhileshverma9978]

Thank you Maam you made it soo easy 👍

[@pritamvyavahare5568]

Your all videos are best , there is no need of to open book after watching ... No need of further Google search , just come on your channel n see videos . Thanku so much for your great help . 😃

[@learnwitheaglestar]

Very informative and easy understanding

[@BaaYo]

Thank you

[@chiragpatel9248]

Superb..👌👌👌👌

[@musicstudio554]

Excellent

[@waqarch8333]

Well explained

[@jaydeepdumadiya2464]

Thank you ma'am ... GOD BLESS YOU

[@abidhussain2484]

mam i like your every lecture... thanks for teach us

[@BaaYo]

Thanks abid..Please share this video with your friends also.

[@dipesh7921]

mam apke to diwane ho gye

[@loardsofcooking4537]

Bht acha

[@zainabqureshi9178]

You're Brilliant

[@sahirahmad7091]

Mam i like ur lectures so much.ur way of teaching is very interesting. Mam please make some videos on basics of centrifugation.

[@justinfaiz1186]

Aslamu alaikum Mam your way of teaching is outstanding. MashaAllah simpler and easier but please include difinitions in your lectures for notes .it will be your kindness .hope you act on it thnx

[@ramchandrasuthar2848]

Great analysis

[@BaaYo]

Thanks Ramchandra 😊

[@darshakeditz9591]

Mind blowing di👌👌

[@ImranAhmad-df3mi]

Nyc explanation mam

[@bingeb3543]

U r superbly good

[@pritipandey6083]

Thank you so much ma'am ❤

[@jaysinha9500]

कौन कौन इसे २०२४ में देख रहे हो ?

[@mridul7820]

Thank you Aarti Mam👍👍👍👍👍

[@BaaYo]

Thanks Mridul 😊

[@NiranjanKathavate]

thankyou maam i am loving chromatography

[@BaaYo]

Thank you

[@quickbiology5159]

Mam you are amazing ❤️

[@saritasharma6557]

Nice

[@aliraza-hc4nw]

mam apkaa method of teaching is very nice mam plz hmray mphil ka exam hn january tk page gel pa video upload krdain thanks

[@mmm-oo5lm]

Everything is good but what is saaperation

[@YogendraYadav_16]

Thank You

[@pushprajpatel1217]

Great mam

[@pitabasamaharana8749]

Why only akta machine only Good and clear information 😊

[@laraibzia8905]

you are best

[@yogitabasnal]

Size exclusion is same as gel filtration, right??

[@sktek370]

Thanking you mam for these videos 🙏

[@priyalgupta9380]

Thank you so much man

[@faizakahloon7961]

v good

[@diadaksh7149]

Thanku very much mam

[@liyaquatali755]

Thanks

[@priyatabassum1190]

really helpful

[@bhatusuryawanshi1089]

Hi mam, your video on size exclusive chromatography is very good. I learn about the importance principle of GPC. Mam can you plz explian about types of detector used in GPC and their working .

[@shivaramsmjadhav3298]

U have not discussed about the type of detector is used in gel filtration/permeability chromatography or size exclusion C.

[@vinaynagar3567]

I like these video and mam you are so cute..😊😊😊

[@harshilsaradhara4908]

interesting video...

[@BaaYo]

Thanks Harshil

[@owaischemist8280]

Mam can you please make a video on type of mobile phase

[@Biotechie_Concepts]

Maam i have a doubt Why Larger size molecules Gives lesser peak and Small size molecules High peak?

[@mrniravpatel9591]

I am analytical developer of GC ,HPLC in Analytical development laboratory at AARTI industries if you have any questions regarding GC , HPLC Application in industries please asked me mam.

[@BaaYo]

sure Nirav. thanks

[@neerajacw]

Thanks for sharing

[@sadiquenirban7299]

Osm mam😍😍

[@sammrayounas2681]

After the separation of large size molecules which component separate first intermediate or small size component of the sample...??? If intermediate eluted after large size molecules then why intermediate eluted first not small size reason???

[@archanapandey7932]

Mam plz make a video on isotope dilution ...my exms are near..plz

[@bhaktibhudiya9983]

Can you upload adsroption chromatography technique video

[@yogeshanjansinger2872]

Very very Thanku mam 😊😊

[@razorr1920]

Just a small question. How does the detector detect the elute sample?