Separation & Identification of Amino Acids by Paper Chromatography| Rf Value Calculation | HINDI

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This video is also available in ENGLISH. Paper Chromatography = Separation of Amino Acids Mixture by Paper Chromatography Technique (ENGLISH) • Paper Chromatography =...
Chromatography is a laboratory technique for the separation of a mixture. The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. The various constituents of the mixture travel at different speeds, causing them to separate. The separation is based on differential partitioning between the mobile and stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation.
Paper chromatography is one of the types of chromatography procedures which runs on a piece of specialized paper. It is a planar chromatography system wherein a cellulose filter paper acts as a stationary phase on which the separation of compounds occurs.
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How to identify which is solvent and which is sample

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In case of seperation of leaf pigments by paper chromatography Does for the rf value we need to take distance from origin to the centre of the band or to the upper side of end of that band.

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