Thin Layer Chromatography (TLC) | Identification of Caffeine by TLC | TLC क्रोमैटोग्राफी

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1. This video is also available in ENGLISH. Thin Layer Chromatography (TLC) = Identification of Sample with Standard Caffeine (ENGLISH) • Thin Layer Chromatogra...
2. TLC Plate Preparation Video- • Thin Layer Chromatogra...
Thin-layer chromatography (TLC) is a chromatography technique used to separate non-volatile mixtures. Thin-layer chromatography is performed on a sheet of glass, plastic, or aluminum foil, which is coated with a thin layer of adsorbent material, usually silica gel, aluminum oxide (alumina), or cellulose. This layer of adsorbent is known as the stationary phase.
TLC plates, preferably ready made with a stationary phase: These are stable and chemically inert plates, where a thin layer of stationary phase is applied on its whole surface layer. The stationary phase on the plates is of uniform thickness and is in a fine particle size. In the process of identification, we have spotted an isolated caffeine sample and compare that with the standard caffeine sample. and calculated its Rf Value.
TLC Plate preparation consists of following steps-
1- Selection and cleaning of the glass plate for Thin-layer Chromatography (TLC)
2- Drying of plate Thin-layer Chromatography (TLC)
3- Making a slurry of silica gel and water Thin-layer Chromatography (TLC)
4- Transfering the slurry into a beaker
5- Transferring of slurry on a glass plate Thin-layer Chromatography (TLC)
6- Uniformly distribution of a slurry
7- Allow the slurry to dry at room temperature
8- Transfer the glass slide on the oven to make it dry
9- Dry and activate a glass plate Thin-layer Chromatography (TLC)
10- Use as per instruction Thin-layer Chromatography (TLC)
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