Some Aseptic Techniques You Can Use In Your Tissue Culture Process

[@steeeebs7286]

Your videos are so helpful. Please keep them coming

[@PlantCellTechnology]

Thanks!

[@eljardindesofi6288]

Gracias por compartir tus videos siempre lo veo y me encanta

[@breehammond3042]

Hi, great video! Where did you purchase your bead sterilizer?

[@Yenty8]

Thanks for the video! what size grow tent are you using as a tissue culture room?

[@h0b0licious]

I'm wondering the same thing.

[@danielmoosonda9941]

Recomiendas algún ventilador con filtro HEPA en específico o cuál usas en este video? Gracias

[@sharolmongrain8022]

Well done thanx

[@PlantCellTechnology]

Hey Sharol. Thanks you so much for appreciating our work!

[@chauyeongphoon2923]

can i know where do you purchase the plastic container? does it have any special requirements?

[@PlantCellTechnology]

PP portion cups from this store. lollicupstore.com/catalogsearch/result/?cat=&q=portion+cups

[@joeygiordani8987]

@@PlantCellTechnology are they autoclavable?

[@siimolen]

is the air filter circulating the air inside the tent? Or does It take in air from outside?

[@PlantCellTechnology]

Is circulating the air.

[@MrWhiltetail]

Doesn't isopropyl alcohol have an oil in it? Wouldn't denatured alcohol be a much better choice? (I know nothing about ethanol)

[@PlantCellTechnology]

70% isopropyl is perfectly fine for tissue culture.

[@rafaelpulido6946]

Veo que también habláis español. He hecho un pedido de ppm y Agar a vuestra tienda , estoy deseando que llegue! Una pregunta, en este vídeo subcultivais una planta de acuario, ¿que medio de cultivo habéis usado? Gracias de antemano

[@PlantCellTechnology]

Gracias, uso 1/2 MS, con 30 g de azucar, 1 ml/l BA, 0.1 ml/l NAA, 3 g de Gallan gum (O 8 de Agar), y 1 ml/l de PPM.

[@rigobertoaguilar8624]

@@PlantCellTechnology cuáles reguladores de crecimiento me recomiendas para generar callo en explante de orquídea phalenopsys y en que cantidad?