Spore syringe vs liquid culture (LC)

[@cryptohound]

I had lc shipped all summer and temp was over 100f and they worked great

[@jakelooter5139]

This video contains a Prodigious quantity of Aspiration...

[@jakelooter5139]

Great content. I used to hate your channel thinking you were a total Newbie but it is apparent that your acumen is actual and more importantly, your research projects demonstrate real inquisitive love of mycological pursuits, which I love, because same kind of interest personally. Very interesting test and honestly this kind of content, as more and more people discover agar, develop proficiency with mycology, will become extremely popular. Thanks!

[@dosisemmanouil]

great video very informative . I have one question.... most likekly the spores will have contam in them, how do you get to grow a non contam mychlium?

[@jcondron]

Thank you for explaining the advantages of working from spores. I was really starting to wonder why anyone would ever want a spore syringe, since it is so much faster and more efficient to work from liquid culture. As a newbie who isn't really interested in breeding new strains, I will go with liquid culture, but it is good to know why so many vendors sell spore syringes.

[@glennplatvoet7111]

Thanks for spore transfer information I'm probably going to use it

[@davideluciano7761]

Make a video about saving agar when drying

[@lunarya3820]

I love how you explain

[@FreshfromtheFarmFungi]

thanks for watching! 🍄❤️🙏🏻

[@FreshfromtheFarmFungi]

thanks for watching! 🍄❤️🙏🏻

[@westcoast8185]

Very well explained for sure! Can you go inoculate from LC to agar? Or is can this only be done from Spore Syringe to agar?

[@FreshfromtheFarmFungi]

yes I do LC to agar regularly - mostly to ensure it’s health and sterility but also to store backups

[@skwest]

If you inoculate (via a spore syringe) a liquid culture container, why are the resulting mycelia not, possibly, multi-phenotype? In other words, how can you be sure that ALL the mycelia in the LC is the same phenotype? Thx!

[@CEOofCTOs]

For newbies, don’t bother with the multi spore syringes (MSS). Go for the liquid culture and enjoy watching your spawn get devoured by chosen mycelium!

[@sunshinevalley0]

Why do I feel like the spores I chose are colonizing much fast

[@houstoner1320]

Nah, spores are fine to start with, just gotta have a little mote patience. Can't always get the live culture you want, and have to go MSS/swab/print🤷‍♂️

[@JAYYBEATS]

But where do I get some quality LC?

[@CEOofCTOs]

@@JAYYBEATS you can't it's illegal because it contains the illegal chemicals :/ at least that's how it is in the US

[@nicolemichele-darkwoodland2136]

@@JAYYBEATS depends what species/strain you're looking for, a lot of mushroom suppliers sell them

[@dpratte]

Hi Gary. I'm an avid and fairly new watcher of your videos, which I find excellent. I just placed an order with you for a few LCs, for the purpose of establishing a outdoor bed, hopefully by mid - October here in Louisville, KY. I have my spawn bags ready, and I'm fighting time. I am looking for your video you reference about fall planting, and desirable species. Can you refer me to that, or alternately, CAN YOU SUGGEST some varieties which you have in stock? On hand I have Stropharia Regusoannulata, Pioppino, Brown Beach, and King Oyster. You mentioned Morel and some others. I'm fighting a first hard frost in December, likely. Thanks, Gary. I love your new house. Gorgeous view. But back to the mushroom topic: I'd like to have three varieties in the ground in 5 weeks. Thanks!

[@familiajackson958]

Muchas gracias! Saludos desde Chile!

[@Marlene5018]

Saludos desde San Francisco Área de la Bahía 😊

[@geezluis55]

Great content! Just purchased some liquid cultures from you :)

[@FreshfromtheFarmFungi]

thanks for watching and for the order! sending out tomorrow AM!

[@hongotopiadada7574]

Can you please activate the subtitles? My girlfriend isn't speaking English that well and we like to enjoy your content together with Spanish subtitles (auto translated) EDIT: We would also like to see spores!

[@CMZneu]

Great video! question, suppose i want to breed a better yielding(or faster,etc) ostreatus strain but only have one strain so it would technically be inbreeding and there wouldn't be any new genetics, what are the chances it would be an improvement compared to cross breeding two different ostreatus strains assuming the successful cross of two genetics? So basically am i wasting my time trying to get something better from inbreeding one strain or is this a viable way and how does it compare to crossing different strains? My reasoning is maybe there is more than enough genetic diversity potencial in one set of genetics for it to not be an issue, what do you normally do? I'm asking because there aren't many different genetics here in my country.

[@sosobk720]

Thank you so much for this information I'm from Algeria watching your videos cuz I love mushrooms

[@SilverPhoenix-PlazmaMoon]

Do you have to use a fruit whose veil has broken to get a spore print, or can you use one whose veil is still intact?

[@WildMycoMycology]

Very informative I learned alot thanks for the content

[@ronbecraft0890]

If I take spore prints from a liquid culture flush, will the spores be identical phenotype? Or does this open up the “genetic library” ? Thanks for the video Gary 👋🏻

[@psst...heyyou6508]

I see different sites listing research syringes and premium research syringes. That seems a bit ambiguous being as they are all for "research". Would these be LC syringes? Thanks

[@FreshfromtheFarmFungi]

research may mean psychoactive ha

[@PokePackPioneers]

I have a good question/idea/experiment would it be posable to make a spore/liquid culture? Instead of making big batch but little ones in the syringe.

[@cuongle-xu3xn]

Hello Fresh from the Farm Fungi, I want to ask how to avoid contaminant from surface of mushroom when collect spores? Or we have someways to remove them from spores? Thank you :D

[@FreshfromtheFarmFungi]

watch our latest cordyceps breeding video this is my favorite method kzbin.infoo2aifWsy68s?si=X6lWdJ-9gT7V5B4G

[@chrishudson4940]

So with that spore print plate do you dispose after 1 use or does it dry out again for reuse?

[@FreshfromtheFarmFungi]

I usually avoid that area and use from an untouched part or let it air dry

[@jamesderoc6717]

which is better for inoculating substrate? great video

[@FreshfromtheFarmFungi]

LC

[@jamesderoc6717]

@@FreshfromtheFarmFungi faster but both will work?

[@oscarm.j1340]

Feelgrabz,..,

[@oscarm.j1340]

Reach him via his instagram handle

[@mattnsim]

Have you got a video where you isolate from a contaminated plate?

[@FreshfromtheFarmFungi]

kzbin.info/www/bejne/nJOwqYikesyVeLM I will do a better one as well once I begin our winter breeding

[@tinbiotech51]

Hello Teacher The spores of the straw fungus (volvariella volvaces) are heat resistant and it can germinate in water? If it sprouts in water, will it keep for a long time?

[@FreshfromtheFarmFungi]

It may be viable in cooler temps - I think a spore print would be a better option to store spores in this case

[@Marlene5018]

@@FreshfromtheFarmFungi So spore print is the best option for most tropical species of mushrooms that will not survive in winter or refrigeration. I love your videos, I’m a beginner trying to learn the most posible 😊

[@josephellis3660]

So Gary, does merging spores reset the clock on senescence?

[@FreshfromtheFarmFungi]

sometimes yes. sometimes the resulting strains are kind of weak - in this case I might go and add another spore from a different mushroom to keep the gene pool strong (different mushroom, same species)

[@maxplank3782]

What if you added a little bit of Honey to the sterile water?

[@FreshfromtheFarmFungi]

it would create a liquid culture. This is a whole different rabbit hole but worth exploring! 🍄❤️🙏🏻

[@ICESTORM667]

How to grow from spore syringes

[@tinman00]

If you make a liquid culture from spores .. is the mycilium have multiple genomics

[@FreshfromtheFarmFungi]

yes I will be explaining my process for that shortly

[@Yvci]

Idk what you are really talking about bc I haven’t grown a myself but what do you think I’m very new but ik what to do do you think a spore syringe or liquid culture is better I just want the mushrooms for myself I don’t care about seeing them grow

[@FreshfromtheFarmFungi]

get a liquid culture from a reputable source for your first grow it will work better

[@lancelootzee5244]

I like the part of the video how you explained how to make liquid culture. 🤷‍♂️🤦‍♂️

[@badseedkramer]

Wouldn't the water activate the spores

[@jeremyshook9429]

I have a four year old syringe. Is it even worth trying?

[@Tommybouy666]

Idk

[@anotherguy9402]

8:30 you can grow it on wood"trips" 🤫

[@MissTEO1]

Do you use gaskets under your mycology lids or do they seal well enough by themselves? Thanks!

[@FreshfromtheFarmFungi]

they seal against the plastic 👍

[@samuelgeorge8524]

Is it always better to transfer multi spores to water agar rather than nutrient agar ?

[@FreshfromtheFarmFungi]

It is debatable but I like to plump them up in water for a few hours before plating

[@samuelgeorge8524]

@@FreshfromtheFarmFungi Thank you. This video is good and just got me thinking. All the best with your new farm! Looking ahead for your outdoor patch cultivation content!!. I've recently been trying it out too with decent success on one of local Indian mushrooms!

[@mohammednazemuddinsiddiqui1071]

How long we can keep cordycep militaris spores syringe like this under 6°C

[@FreshfromtheFarmFungi]

Im not sure about those I assume they will be viable for a long period but I don’t have enough experience with it myself

[@timothy4016]

What happens if I inject a spore syringe into a liquid culture?

[@FreshfromtheFarmFungi]

you can create a multispore/polyculture solution but it has a high risk for contamination

[@danwilmott3918]

Soo, I have my licqad culture, in the fridge, is that to cold

[@FreshfromtheFarmFungi]

no it should be fine as long as it doesn’t freeze

[@CDCLLC2023]

What is, close to sterile water?

[@FreshfromtheFarmFungi]

sorry for the subjective description- I would say less than 10CFU/mL would be a good starting point but I got lazy 🙂🍄❤️

[@glenw3814]

Good stuff 👍🙏🍄💖

[@oscarm.j1340]

*Check out this dude i get my stuff from, he's got Magic Mushrooms, Chocolate Bars, Lsd, DMT, MDMA, Spores, Syringe, Grains, and other psych's, 🍄💯 he could direct you also if you're a newbie in it,,,*

[@oscarm.j1340]

Feelgrabz,..,

[@glenw3814]

@@oscarm.j1340 Please don't post your spam here. You put me in the awkward position on of appreciating your offering, but despising your spam. If you want to advertise make a channel.

[@jakelooter5139]

I highly doubt you will encounter significant variability among stropharia rugosoannulata TBH I would probably also though use the multi spore to force more dominant phenotypes for your specific microclimate to outcompete others though...that said probably not much genetic variability speaking from extensive cultivation experience and extensive experience collecting this species in numerous locations in field planted and wild, as well as long term grower of it and expert biocultivator. what would be interesting though would be to see if you can fruit it indoors and or create an albino or leucistic version of it? See if you can fruit it indoors would be more fun,....this is the most aggressive mycelium I have ever seen in real life it can colonize underground like 100 feet a month according to research. Also to any people reading who havent tried it imagine a Portabello but about twice as good. the "wine" segment of the name refers to the burgundy coloration of the cap top (pileus) but also to the flavor, which tastes like, at least pan sauteed properly (meaning perfect french gastronomic technique not by amateurs) it has already been deglazed with wine when it has not actually been. these are way better than Agaricus bisporus flavorwise, while texture is similar. shape is NOT very similar I am just using A. bisporus as a relevant example because most people are familiar with this species, and when weighing the options to grow versus not it needs to be known this is significantly better than your most common grocery store option. the downsides to this species is that your fruiting is not wide range, meaning, does NOT fruit year round. it is ephemeral but can be dried easily and picked huge. grilled they are fantastic. I grow shiitakes Japanese style and have them virtually year round and my shiitakes taste way better than sawdust block grown grocery store ones and I pick wine cap over shiitakes, even the 50-100 USD per LB log grown shade grown ones I have (market price for quality in Japan NOT here, but it isnt the same product so price is not relevant comparatively speaking). Spawn location doesnt matter and it colonizes rapaciously easiest to grow mushroom I have EVER grown except for Pleurotus pulmonarius that stuff is weedy as hell and tastes like licorice (JUNK) but is a great newbie project, as is Agaricus blazeii spawn planted in soil in pots in chunks (does better broken into like silver dollar sized versus completely broken up extensively tested and VERY easy if you have spawn and the temps of the soil are over 70 F).

[@Mitzi712]

Is there a reason why injecting a spore syringe directly into a liquid culture is not much talked about?

[@FreshfromtheFarmFungi]

kzbin.info/www/bejne/Y3i6iYl9jt95qZY it’s the same principle here

[@JosephKeenanisme]

Another good one. The way I think of spores is that they are a cross between sperm and eggs in animals. They have to hook up with another spore to get going.

[@johnriperti3127]

no sound ?

[@FreshfromtheFarmFungi]

there is sound

[@johnriperti3127]

@@FreshfromtheFarmFungi yes now, probably it was still uploading. Thanks, good stuff

[@themyceliumnetwork]

I have sound, update your browser or try to reboot

[@jakelooter5139]

For the love of baby jesus, put an inoc port on your sterile water jars! people drill a hole and stick a stick on self healing injection port DONT open your jars unnecessarily please! Come on bro the jar is full, even w a 1.5 inch needle that would be super easy to pull from...Maybe this is a sloppy technique yet good results game flex????

[@whoelseisdeadinside]

Such bad audio bro fix your mic lol

[@TacosGaming]

I just shot a spore syringe into grain, was that a bad idea? What would be the correct approach?

[@FreshfromtheFarmFungi]

It may work if the spore syringe is clean but watch this video too kzbin.info/www/bejne/Y3i6iYl9jt95qZY