ImmunoHistoChemistry (IHC) - Video Protocol Series

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Immunohistochemistry (IHC) refers to the process of detecting antigens (e.g. proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. IHC takes its name from the roots "immuno", in reference to antibodies used in the procedure, and "histo," meaning tissue (compare to immunocytochemistry). The procedure was conceptualized and first implemented by Albert Coons in 1941.
Immunohistochemical staining is widely used in the diagnosis of abnormal cells such as those found in cancerous tumors. Specific molecular markers are characteristic of particular cellular events such as proliferation or cell death (apoptosis).
Immunohistochemistry is also widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.
Visualising an antibody-antigen interaction can be accomplished in a number of ways. In the most common instance, an antibody is conjugated to an enzyme, such as peroxidase, that can catalyse a colour-producing reaction (see immunoperoxidase staining). Alternatively, the antibody can also be tagged to a fluorophore, such as fluorescein or rhodamine.

[@akshayvyawahare6296]

Very nice explanation.

[@clg1711]

Well illustrated. Thank you Team StJohnsLab. Audio quality could have been bit more distinct.

[@zahrlov]

Excuse me, i am miss something or u didnt use decloaking chamber? Thank you, best regards

[@priyankajain4455]

Hi Is the first step applicable if the slides are positively charged?

[@rajeshnimbu3018]

Great sir

[@totAli-mm1jq]

ماهي مدة ضهور نتيجة اختبار IHC؟

[@DoctorJPTV]

Our tissue sample kept slipping off the glass slide. What should we do to prevent it from happening?

[@azizfatimahussain8944]

Use TOMO slides

[@Prasad-io4jf]

I want Telugu

[@user-cs3gy3eh1p]

I go to a school named IHC

[@MalashettyIndi]

i want to kannads

[@donvin.5921]

Nice video. Can you please try to be a little audible in subsequent videos. Many of your Audience are from third world countries and have to strain their ears to understand what you're saying.

[@anusmananushu1969]

Ihc

[@jyotikumbar8048]

Which marker pen is used for the prevent the antibody flowing on the silde.?

[@frenchfryequeen]

@@jyotikumbar8048 I think any hydrophobic pen would work

[@avanaavan2575]

The music is annoying!!

[@user-fk5ql8fy4z]

일 똑바로해라 헐박사야? 장난하냐?

[@BlizzyFoxTF]

Desellation??? BS