#StudentsCanCreate

[@bikilahordofa6165]

Thank you Very i had learnt so many think from your video.

[@tessacorsetti1410]

This video seems to have a lot of great information, but please consider having the music stop playing the background while you're teaching. For those of us that are hard of hearing, it makes hearing the lesson that you are teaching extremely difficult to understand.

[@sciexpos]

I m sorry for that at that time I was fascinate about music 🎶

[@ayeellapradeepgoud3377]

Good information sir👍 Keep going on ..

[@JuanJo_Sierra]

Great video!!

[@dominiquevargas4364]

thanks for the tutorials

[@gamersarkar9995]

dear make some videos on binary data like RAPD

[@saniilanah9470]

Sir, your videos are so informative, easy to grasp and thank you for letting me to learn. I had a query, i have installed GenAIEx, used the same tutorial data format but no execution whatsoever when I try to analyse. what could be the reason? kindly help me. thank you

[@sciexpos]

Data set is ok I think you need to check first two rows and columns. Where need fill up genotype and markers info for that please check geneAlex data file. And one more please save excel file in 97-2003 format i. e .xls not xlsx

[@NasirHamad]

hi sir i hope ur doing great ,,,,how can prepare input file for amova analysis plzz guide me sir

[@sciexpos]

The AMOVA video already on the channel. Explain how to analysis. Best luck

[@NasirHamad]

@@sciexpos yes sir but I don't know how to prepare the input file ....I have 518 sequences in fasta file how can I prepare input from that

[@NasirHamad]

@@sciexpos yes sir but I don't know how to prepare the input file ....I have 518 sequences in fasta file how can I prepare input from that

[@sciexpos]

For DNA seq. Data AMOVA analysis Search Arlequin Software online follow the user guidelines instructions. Best luck

[@NasirHamad]

@@sciexpos okay thank you sir

[@kukzzktara4925]

Is Microsoft Excel 2019 supports GeneAIX 6.51?

[@sciexpos]

Yes it will support

[@ajinkyamandave1965]

which method used for calculate genetic distance

[@sciexpos]

You can use any one. I m preferring nies genetic distance new one

[@rizwanasyed14]

nice ppt, may i know more information i have SNP data and from this how to make input input file. thanks in advance

[@Yostena2018]

Your videos are highly informative. But how to analyze SSR amplified tetraploid and hexaploids using GeneAlex and powermarker softwares? Is it possible to analyze both of them at once or separately? Can you please suggest me? Thank you!

[@sciexpos]

As per my knowledge you can analyze by using Ntsys and GGT software which can handle binary data set.

[@monpihazarika7510]

i have a binary data set scoring as o for absent and 1 for present..while calculating nit shows 100% among population .. suggesting to help me to solve the issue regarding analysis

[@sciexpos]

Check my previous videos on how to prepare data input file formats. Or try to copy n past as numeric on another sheet.

[@kukzzktara4925]

Sir 1. Nei's genetic distance can be calculated by which software? 2. please make a video on constructing a dendogram using ssr marker in MEGA X software

[@sciexpos]

Watch my Pwoermarker analysis video in that I am detailed explains how to do that

[@kukzzktara4925]

@@sciexpos plz make a video on MEGA X software too. Power marker has limitations.as.it cannot be installed in our personal laptop

[@kukzzktara4925]

@@sciexpos oky I'll watch power Marker video

[@gongtae7836]

Hello.. i use 60 accessions on my research using rapd and srap markers.. for each accessions i only use 1 plant and one time pcr.. so how to analyse amova?

[@sciexpos]

As far as my knowledge concerned rapd amplify more than two alleles. If you manage to score max two alleles in each genotype then it is possible to do so. Try to prepare data was shown in video and follow the steps I think it will work. All the best

[@tabithalomotey2984]

Thank you.

[@userq58_436]

Sir , can we install this software on MS office 2021

[@sciexpos]

No need to install this software, you can use exe file to normal use

[@drnavneeti]

Sir why I am getting heterozygocity and fixation index vale 0 ??

[@sciexpos]

Your marker should be polymorphic in population.

[@drmuhammadabaidullahlally8526]

@SCIEXPO please help me how can i adjust missing values in data sheet

[@sciexpos]

Follow the instructions in videos and manuals. For SSR genotyping for the missing scoring I will suggest repeat pcr. If gene specific markers go through the standard band size.

[@nguyenhuunhan284]

How calculate blup in genealex 6.5?

[@sciexpos]

I will see it Thanks

[@twoworld5707]

Is it possible to analyze if i only have one individu on some populations? Pls help me

[@sciexpos]

Yes you can do it

[@twoworld5707]

@@sciexpos i tried to update project but it failed and showed that i cannot open the file blablabla .tree, do you know how to solve it? I already have R program

[@sciexpos]

Which program did you used and what exact analysis want to do?

[@ssthapit]

Wanted to watch but the music was distracting and could not follow

[@sciexpos]

Noted

[@kukzzktara4925]

Sir I tried to run distance and AMOVA, but no progress. What are those 1, 12 2, 6 ... In first row

[@kukzzktara4925]

I'm working on musk melon, so should my population be reticulatus as 1, conomon as another and so on?

[@kukzzktara4925]

I tried many time but its not happening. Can you kindly give me your email ID or contact address

[@sciexpos]

Watch my video I all mentioned in that

[@sciexpos]

It is or population information

[@kukzzktara4925]

Thankyou sir. It's very informative

[@harishbjadhav6844]

How to confirm p is significant or not

[@sciexpos]

As far as my knowledge concerned the 95% (0.05) is a significant value used for as general analysis. But in case of the analysis of genetic association studies, a parameter of statistical significance, a P-value, is used to determine the certainty of an association. The significant p value should be less or equal to 0.05. Most minimum value should be given first priority.

[@khaledsalem4297]

Dear Dr., Greetings, I hope that you could help me in calculate data from 0-1 matrix and estimate the following mean number of alleles (Na), Shannon diversity index (I), observed (Ho) and expected (He) heterozygosity and inbreeding coefficient (F) and AMOVA. Hope know file format and upload data using genealex and I have office 365 apps waiting your kindly reply

[@sciexpos]

I will suggest to you covert your data into base paired format. For file format you can watch my video. One more while converting alleles in each genotype consider max two allies based on band intensity.

[@khaledsalem4297]

@@sciexposMy office is 365 apps and I could not find the Genealex icon in excel file. Are their any suggestions?

[@sciexpos]

Check Manul of GenAlex compatibility and enables macro settings I hope it works and one more go to the add in top tab inside in left top corner is GeneAlex is there and click and enjoy

[@khaledsalem4297]

@@sciexpos I have tried but could not success could send me simple steps show how could download and solve these troubles

[@sciexpos]

If possible Try to use less version of office 2007,2016 it will work

[@andrewsearle6050]

Please remove music from your future videos. I find it difficult to hear what you are saying over the music.

[@andrewsearle6050]

my favorite part is between 08:00 and 08:10 when i can hear your voice lmao

[@haikalarsyad5957]

9P