RVD stands for Repeat Variable Diresidue. The amino acids in the middle are called central TAL targeting domain.
@xavierwong50442 жыл бұрын
Watched your video on ZFNs and CRISPR as well, has helped a heap with my assignment. Thanks you for the great videos 👍
@GenomicsGurus2 жыл бұрын
Great to hear Xavier. Best wishes for your studies 👍🏼
@anirakbenadam1124 ай бұрын
Your explantions are very clear, I enjoyed your videos and learned a lot. Thank you very much!!!
@jackpertzel4 жыл бұрын
Extremely helpful making my assignments much easier Thank you!!
@GenomicsGurus4 жыл бұрын
Glad you found it useful Jack. Check out our other genome editing videos.
@brandonunger84394 жыл бұрын
Really enjoying this series of videos Thank You
@GenomicsGurus4 жыл бұрын
You're very welcome. Glad you're enjoying them!
@stanleysalivan75873 жыл бұрын
Great video; Shortly and very clearly explained. Thank you!
@GenomicsGurus3 жыл бұрын
Thanks Stanley. Glad you found it useful!
@TheDRNA3 жыл бұрын
Great video, detailed yet short thank you
@GenomicsGurus3 жыл бұрын
Glad you found it useful!
@caiorodriguescorrea4973 жыл бұрын
Thank you for the very helpful video and good job on the channel!
@GenomicsGurus3 жыл бұрын
Thanks for your kind feedback. Hope you enjoy the rest of the videos!
@carissejadeangulo203 жыл бұрын
Extremely helpful in making my topic discussion for one of my plant breeding subjects. Thank you so much sir!
@GenomicsGurus3 жыл бұрын
Glad it helped. Best wishes for your studies!
@carissejadeangulo203 жыл бұрын
@@GenomicsGurus Sir if I may. Does "predictable specificity" pertain to the exception of the 12th and 13th amino acids in the repeat?
@GenomicsGurus3 жыл бұрын
Residues 12/13 in each repeat are the variable amino acids that make contacts with the bases and thus direct specificity
@karolinawasilewska17483 жыл бұрын
Very clear and helpful presentation. Thanks you so much :))
@GenomicsGurus3 жыл бұрын
Thanks Karolina. Glad you found it useful.
@mohammadhammad89082 жыл бұрын
ITS REALLY A GOOD PRECISE AND HELPFUL VIDEO BE BLESSED
@GenomicsGurus2 жыл бұрын
Glad it was helpful!
@deepikakumar1430 Жыл бұрын
it is so good ,very helpful
@akashphukan6452 Жыл бұрын
Thank you
@johannacatalinaroseroroser69933 жыл бұрын
Thank you you so much! :)
@GenomicsGurus3 жыл бұрын
Glad you found it useful!
@halihammer Жыл бұрын
Very nice images!
@kiara_l87323 жыл бұрын
is this method susceptible to off target cleavage like with CRISPR enzymes?
@GenomicsGurus3 жыл бұрын
It is more specific than CRISPR, but all nuclease technologies have a degree of off-target activity. This introductory talk briefly mentioned obligate heterodimer mutations. These greatly reduce off-target cleavage arising from binding to only one half-site. With these FokI mutations, TALENs are arguably the most specific platform. Specificity isn’t the only issue though, new technologies like prime editing, which avoids creating a DSB, also reduce unwanted repair outcomes. See my talk on that...
@kiara_l87323 жыл бұрын
@@GenomicsGurus thank you for the help! :)
@jamesraymond1158 Жыл бұрын
Excellent! I finally understand it. However, one question: at 3:40 where the TALENs are biding to the DNA, it seems that the TALENs are too bulky to fit close enough together to bind to successive nucleotides.
@GenomicsGurus Жыл бұрын
The successive domains curve round the the DNA helix like a propeller - it's an amazing structure