TALENs - TALE NUCLEASES - GENE EDITING EXPLAINED!

[@qwerty00000hghgmhvmgh]

RVD stands for Repeat Variable Diresidue. The amino acids in the middle are called central TAL targeting domain.

[@xavierwong5044]

Watched your video on ZFNs and CRISPR as well, has helped a heap with my assignment. Thanks you for the great videos 👍

[@GenomicsGurus]

Great to hear Xavier. Best wishes for your studies 👍🏼

[@anirakbenadam112]

Your explantions are very clear, I enjoyed your videos and learned a lot. Thank you very much!!!

[@jackpertzel]

Extremely helpful making my assignments much easier Thank you!!

[@GenomicsGurus]

Glad you found it useful Jack. Check out our other genome editing videos.

[@brandonunger8439]

Really enjoying this series of videos Thank You

[@GenomicsGurus]

You're very welcome. Glad you're enjoying them!

[@stanleysalivan7587]

Great video; Shortly and very clearly explained. Thank you!

[@GenomicsGurus]

Thanks Stanley. Glad you found it useful!

[@TheDRNA]

Great video, detailed yet short thank you

[@GenomicsGurus]

Glad you found it useful!

[@caiorodriguescorrea497]

Thank you for the very helpful video and good job on the channel!

[@GenomicsGurus]

Thanks for your kind feedback. Hope you enjoy the rest of the videos!

[@carissejadeangulo20]

Extremely helpful in making my topic discussion for one of my plant breeding subjects. Thank you so much sir!

[@GenomicsGurus]

Glad it helped. Best wishes for your studies!

[@carissejadeangulo20]

@@GenomicsGurus Sir if I may. Does "predictable specificity" pertain to the exception of the 12th and 13th amino acids in the repeat?

[@GenomicsGurus]

Residues 12/13 in each repeat are the variable amino acids that make contacts with the bases and thus direct specificity

[@karolinawasilewska1748]

Very clear and helpful presentation. Thanks you so much :))

[@GenomicsGurus]

Thanks Karolina. Glad you found it useful.

[@mohammadhammad8908]

ITS REALLY A GOOD PRECISE AND HELPFUL VIDEO BE BLESSED

[@GenomicsGurus]

Glad it was helpful!

[@deepikakumar1430]

it is so good ,very helpful

[@akashphukan6452]

Thank you

[@johannacatalinaroseroroser6993]

Thank you you so much! :)

[@GenomicsGurus]

Glad you found it useful!

[@halihammer]

Very nice images!

[@kiara_l8732]

is this method susceptible to off target cleavage like with CRISPR enzymes?

[@GenomicsGurus]

It is more specific than CRISPR, but all nuclease technologies have a degree of off-target activity. This introductory talk briefly mentioned obligate heterodimer mutations. These greatly reduce off-target cleavage arising from binding to only one half-site. With these FokI mutations, TALENs are arguably the most specific platform. Specificity isn’t the only issue though, new technologies like prime editing, which avoids creating a DSB, also reduce unwanted repair outcomes. See my talk on that...

[@kiara_l8732]

@@GenomicsGurus thank you for the help! :)

[@jamesraymond1158]

Excellent! I finally understand it. However, one question: at 3:40 where the TALENs are biding to the DNA, it seems that the TALENs are too bulky to fit close enough together to bind to successive nucleotides.

[@GenomicsGurus]

The successive domains curve round the the DNA helix like a propeller - it's an amazing structure

[@subhojeetroychoudhary6364]

Boka_____ amake aro guliye dilo

[@ahmadnaveed170]

SIR PDF AWABILE THIS LECATURE

[@GenomicsGurus]

There is not.