WHAT IS CRISPR? - GENE EDITING EXPLAINED!

[@Joseph_C4JB]

Absolute legend, massive thanks from a 3rd year struggling on with his Genetics dissertation with only 1.5 years of in person teaching (strikes + a pandemic are a mean combo).

[@GenomicsGurus]

You’re welcome Joseph. Good luck with your studies!

[@praneetasrivastava1919]

thank you so much for the genome editing series.

[@GenomicsGurus]

You're welcome. Glad it was useful

[@fabianperson]

Hi! Thanks a lot for the great lecture series about genome editing! It helps a lot to understand the topic! At 2:38 , do you mean double stranded RNA sequence instead of DNA? Greetings from Berlin!

[@GenomicsGurus]

Thanks Fabian. Well spotted. Yes, dsRNA of course. Best wishes for your studies!

[@christiannissen2381]

Thanks for sharing your knowledge! What does PAM stand for?

[@GenomicsGurus]

Glad you found it helpful Christian! Protospacer Adjacent Motif - the protospacer is the first ~20 bases that match the genomic target. The PAM is the next 3 bases in the genomic DNA that follow after the protospacer target, which Cas9 locks on to.