ZINC FINGER NUCLEASES - GENE EDITING EXPLAINED!

[@zeinabaltoufaili5791]

I am working on my paper analysis project for my undergraduate genetics course and this playlist has really helped me in grasping new concepts. Thank you so much for making this knowledge available to everyone.

[@GenomicsGurus]

You are very welcome. Best wishes for your studies. Stay safe, GG

[@sebastiansaavedra2149]

Great explanation of an older--yet somewhat complicated-- technique. Really appreciate it !

[@GenomicsGurus]

Glad you found it useful. ZFNs still have merit as they are very small and easier to deliver in viral vectors.

[@nikaudymond5874]

This has been really helpful for my chemistry assesment at school. I personally found it very interesting and informative as well.

[@GenomicsGurus]

Great! Good link with your assessment

[@jennamuhlberg6762]

These videos were perfect. Concise, thorough and incredibly helpful. Thank you!

[@GenomicsGurus]

Thank you Jenna. We're pleased that you found them to be useful!

[@renee_chu]

Super helpful before my genetics exam! Thanks!

[@GenomicsGurus]

Glad it was helpful!

[@huonghuong9586]

so touching for an excellent video

[@GenomicsGurus]

Glad you think so!

[@erfan74ir]

Thanks for the great resources you made. Very helpful :-).

[@crisprtalk6963]

Excellent! Thank you

[@GenomicsGurus]

Glad you enjoyed it!

[@shivamsingh21]

Superb concept So much Thank

[@javiervalls5311]

Hello, what I don't fully understand is how by changing some aminoacids from the ZFN domain (each square) it is possible to recognize a specific trinucleotide.

[@GenomicsGurus]

Hi Javier, I suggest that you read reference 6 listed in the description or some other articles on zinc finger specificity. Best wishes

[@RichardLee-c5z]

Tremblay Drive

[@guixien]

This is incredible and invaluable, thank you. Just curious, when you mentioned that zinc finger arrays are small, I guess you imply that they are smaller than TALE and Crispr Cas 9 proteins. Without worrying about how the zinc finger arrays are made, does this "small" genome editing tool imply that the tool can be more effective in general, or are there cases such that only small enough tools like zinc finger array is effective?

[@hughspotswood5702]

Good stuff Adam. I remember meeting you in Glasgow when I was in sales at Millipore. Now I work for Sangamo (via Sigma)!

[@GenomicsGurus]

Thanks. Hope everything is going well with you

[@laravandermerwe526]

Busy with my Honors in Biochem - this is pretty helpful

[@GenomicsGurus]

Thanks for the feedback Lara. Hope you find the rest of the series just as helpful!

[@christuohino3960]

I really hope there's a Fok2 lol

[@RoseAnderson-q1e]

Moore Shore

[@ElvisManni-v3o]

Bartoletti Course

[@Akanshanarang]

What's the colour pf zinc finger protein?

[@ibelmerishanongpluh2365]

Gene and genome editing is the same or not???

[@GenomicsGurus]

'Genome editing' would include genes, gene regulatory regions, and other non-coding intragenic regions, so is just a broader term.

[@ibelmerishanongpluh2365]

That means it's more or less the same thing right

[@GenomicsGurus]

The editing process is the same, the intended target is different

[@ibelmerishanongpluh2365]

Ohh okay thank you so much ☺️

[@ashutoshtiwari3098]

Please help me sir do you tell me what is the source of nuclease in saliva of human

[@GenomicsGurus]

Goggle it! 😀

[@jayshreeram24572]

Now crisper cas 9 available

[@GenomicsGurus]

Indeed. See our videos on them. ZFNs still have value as they are small proteins, which is good for viral vector delivery

[@RobertRowell-o6y]

Ernser Rest