The art of perfect agar transfers | Using inoculation loops for LC's

[@amospena2046]

Video starts at 4:55 your welcome

[@Haraharabole]

Thanks for everything you do keep up the good work

[@WillysWorld]

Thank you guys for the love and support!! Down below you'll find links to the private library and much more! Private library, step by step videos, private chat & Reddit 👇🏼 www.patreon.com/willymyco Social media links 👇🏼 Instagram ~ instagram.com/official_ttf_willy (Backup account) instagram.com/ttf_willy Twitter ~ twitter.com/willy_myco If you would like to share your breakthrough story, please send a brief summary over to [e-mail] Music by: D'rok the menace

[@sydneyhunt6681]

Love watching videos on brake through sooooo interesting stuff 👾🥰

[@paulrod81]

Thanks again. Always spreading knowledge.

[@awakenedfate7568]

Thanx excellent information liked and subscribed 👍

[@fullspectrumalchemist]

I just got inoculation loopand my first real spore prints,just waiting on my new laminar flow hood (bonsai 12x12) before taking the prints out the package

[@stefaniejoy2034]

I'm trying the bucket method on Blue Oysters and I did it wrong on pine with lol 7 buckets. Live & Learn. So trying again with different buckets and Aspen wood. Wondering if dumping 5 galleons of boiling water on the Aspen shavings and letting them cool or letting them cool till cool enough to handle is the right thing to do. Kinda murky on the difference between pasteurization and Sterilization here. I know milk get flash heated to almost 300F then quickly cooled down and that denatures the proteins and I'm thinking that is Pasteurization. But Sterilization is long term hard core High (boiling hot) heat to kill everything. Right? This is my first foray into growing via the bucket method. I just bought some of your brown treasure and then going to try that with a popular dung loving species. Getting my feet wet here. Grateful for all you do. So passionate, fun, generous, easy to understand and downright awesome and KIND.

[@TheIndianaOutlaw]

Many people are using the Tovolo Strawberry Huller available on Amazon

[@CELERYLAWN]

I looked it up and it seems pretty good for the job it would call for, imo it’s probably better than the straw

[@Theisolationclub]

These are PERFECT!

[@kevinshepherd6306]

Metal straw guys

[@kellyeldershomesteadbeginn8242]

Willy is legit a teacher. Love how you speak plain English for the newbie.

[@MysticNorthwest]

Thank you for the video, is it possible to put agar cut into liquid culture medium to let the mycelium grow to make lcs?

[@Allaboutpie]

I was gonna make my own flow hood. I was gonna put a ultraviolet light inside the box and turn it for about ten or fifteen minutes before work. Does this sound like it would help keep contamination down? Looking for an opinion on if this is a good idea or a waste of time and money. I haven't seen this done yet?

[@jobrrrahman298]

Hello! I have been following you no one els. I build your mono tub I bought your substrate. I not getting any fruit. I didn’t mix anything substrate. Do I have to do anything to your brand substrate? Every day when I go check on it the substrate is completely dry I am checking my mono tub it’s working the way it supposed to be. Is any way you go over your mono tub see if it’s anything different from the video please. Thank you

[@Broc971]

I’m aspiring to dedicate myself to mycology I’m kinda broke rn but I will be joining your patreon man thanks for all the free info and I will be supporting your patreon as soon as I can!!!

[@shawnmagby4399]

Best 20 you can spend

[@corneliuscross7873]

Does anyone have a link to Willy's catalog of old videos? Can't find it on the Patreon and need to go back to basics on a particular subject.

[@gordotek]

Do not swipe your agar plate all over the place like Willy, keep one small inoculation at the center of the plate (you want the culture growing outward from a single point, and you will take cuttings from the outer edge of growth before it hits the side of the plate). And its really not very hard to use a sterile (flame sterilized) xacto knife or scalpel to cut a small piece of agar out, practice on a spare agar plate, takes 5 minutes to learn to do it easily. Using a hole punch seems a bit ridiculous to me and its going to be harder to keep that clean (vs. a scalpel) and it probably won't be as sharp.

[@Pope-Hope]

Aye, I think I watched a how-to video by you on DMT extraction. I can't find it anymore

[@SouthernScaffolder88]

@@Pope-Hope it’s on odysee

[@ChilSc]

Not suprised that Willie doesn’t respond to any comments

[@arbarb7204]

Notice folks he uses 70% alcohol 90 %good for electronics because it evaporates faster.

[@stefaniejoy2034]

Hey Willy, awesome Edu here. Appreciate all your enthusiasm, tips, tricks, passion and love! So ya a question? Can you explain the difference between Pasteurization and Sterilization? inspirations

[@jessicahay9305]

Sterilization kills everything living in/on something. No bacteria, mold, microbes etc. Pasteurization kills most of it but not all.

[@Gottiline_Ace]

Are there really people who care about perfect circular transfer cuts? Boy, I am slacking big time. 😂

[@mattb2544]

fr, like sooooooo many. Yes it looks great but makes 0 difference. now their selling $30-$40 'myco punches" yea theyre neat and cool but why.....

[@mengelman2]

cant find you on patreon

[@billprice6458]

Skills.

[@kellyeldershomesteadbeginn8242]

What's the name of the song that always plays on your videos?

[@ericpoe2694]

Man all I can say is thank u I'm fixing to bring in my first harvest and it's all because of u

[@ericpoe2694]

@STEPHEN_.SPORE_ON_INSTAGRAM I looked up that Stephen name on insta and nothing popped up I not sure if I looked it up right

[@mikeflanigan1201]

Man so that really amped wire’y guy that’s always pushing that shopping cart is using a “agar punch” riiiight lol

[@andrewmodell5889]

Yea he needs to smear .and that loop isn't used like that it has a cutting edge to scoop out the dish it makes a little scoop thrn you can just transfer it but I'd definatly smear soci could see growth and have wider parameters to transfer he over top him self and talking lc should be done with a syringe it's it's off a slant I'd definatly use a swab ...and a little multiplayer like a soy peptide ...squiggle is right he knows his stuff but you could use the centerdudge bendil and Swerell like a lc and thrn swab off that bit remember sterile teck...

[@andrewmodell5889]

Youmcsn get those loops for those circles and that looks shaky

[@sydneyhunt6681]

How long can new growth last before you need to make new dishes????????

[@legendno1]

60 days...and more. depends on how well you keep them, temps, mixtures etc etc❤

[@cameroncook975]

Love your content bro, I think the first videos I watched before ever starting. But, I gotta say, I was freaking out inside every time you set your dish lids in front of your dish. Should always open and move away from your airflow, don't work between the filter and your open plates.

[@lukemartin6927]

No parafilm use grafting tape works better.

[@WillysWorld]

Try Budding tape, it also works really well

[@BrainboxOfficial_1]

Hit up 👆 the handle. He ships🍄💊🍫....

[@austinlink7817]

@@WillysWorld grafting = budding tape

[@lhicks1354]

I wanna see that spreadsheet. Also, much better at doing less talking and more showing.

[@BrainboxOfficial_1]

👆 👆 Legit plug 🍄

[@NativeSteelPlayer]

I would love to watch your content, but that mask of yours Has to go!!

[@furrsync4522]

Maybe you should not be in this field.

[@FBIcounterscammerdepartment]

Does he own milly wyco?

[@1993whitenoise]

No two different people. I had the same question a few years back.