This microbe explodes

You will be able to see what happens when a 3% solution of hydrogen peroxide is added to water microorganisms. Hydrogen peroxide is a strong disinfectant that can cause microbes to explode due to oxidative stress. Observe the effects of hydrogen peroxide on these microorganisms and learn about the process of oxidative stress. [Video 251]
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#Microbes #OxidativeStress #HydrogenPeroxide

[@datenraffzahn6094]

This is impressive, I'd imagine not many people have seen the effects of disinfectants outside of a lab. I don't know if its possible, but seeing the effects of antibiotics like peniciline or something would be very interessting. In any case, thanks for the video!

[@eugenetswong]

Yes, penicillin would be a great idea. Various medicines would be very interesting.

[@johnschuster1770]

I wish that I had you as an instructor when I took my microbiology class. You explain and demonstrate this topic very well.

[@johns9652]

When I was a kid, I saw chickens being killed and prepped for the first time. It was a revelation to me then, to actually see a "chicken running around like it's head was chopped off". I was reminded of that by the spiral motions of the organisms on the slide.

[@grimsas]

If only my biology lessons would be that interesting. I wanted to study pharmacology at university, but the lessons at school were so boring I stopped going to them and ended up in IT and electronic engineering...

[@leepride7978]

Waterplants use a combination of vinegar and hydrogen peroxide to sanitize their filter chambers. The walls and troughs are sprayed vigorously and thoroughly.

[@jrobertfreeman990]

Most interesting! I am writing a report on the dirt, grit and grime that are a concern for the car wash industry. Do you have a video of some grime that you can direct me to. I would greatly appreciate the help. I have found many pictures of dirt and grit but not of "grime". The microscpic world is an amazing and scary place that is all around us and goes unnoticed. Thank you for sharing and keep up the good work Sir.

[@Microbehunter]

The "grime" that you refer to is called "biofilm" in biology. Biofilms can be found where water (or even oils) are contacting a surface over a longer time. In your household, check the bottom side of the drain plugs (in the bathroom) they feel slimy. This is a biofilm. Also in a lake, rocks feel slimy and this is a biofilm. Bacteria (etc) will start to grow on the surface. Biofilms are a huge concern because they can cause the blocking of pipes and tubes. Even in the oil industry biofilms have caused problems, where there were microorganisms growing on surfaces (of tubes, valves etc) using the oil as a food source. So you might want to check those parts of a car where there is liquid (cooling water, etc). Other interesting places to check and clean are the air filters (ventilation and air conditioner). When these filters are clogged, then they can not remove particles such as pollen anymore. People who are allergic therefore have a problem. This is why air filters should be exchanged frequently. Not only the filter, but also the inside of the tubes carrying the air there is a dust deposit. And then there is of course the carpet. Large surface area for organisms to grow in - a true ecosystem.

[@glassbackdiy3949]

I think that's the best picture of a ciliate I've ever seen! I have serious scope envy! it puts my 1970's Olympus FHT to shame even with the phase contrast set-up on it! I've been trying to isolate different photosythetic bacteria from a Winogradsky column but I think the summer temps have been too high for my first few attempts, or my agar recipie is off (PDA/Molasses mix).

[@SimplyElectronicsOfficial]

I'd imagine they spin because the cilia are affected on one side before the other?

[@burningoyster]

More of this please: 70% IPA, 70% EtOH, Bleach, Umonium, gentamicin, pen/strep, ... Would be very interesting to see the differences in real time

[@peterashcroftq1494]

Hi sir, love your videos, I’m a cleaner and we have two options to clean with; bleach and hydrogen peroxide. We predominantly use bleach but it leaves residue on the surface we are cleaning, the peroxide doesn’t. I’m wondering which product would be more effective in destroying any germs or bacteria?

[@theanalymous]

Bleach is not good for your lungs, I would avoid it if you work with it everyday.

[@张腾飞-y1n]

The objective lens with large NA is the key factor of the dic effect, because the amount of light entering is larger, it allows you to adjust the dic prism closer to the pseudo dark field, the shadow and highlight ratio is closer to one to one, and the stereoscopic feeling is stronger and the effect is better.

[@originalguy4535]

Make video on benzalkonium chloride vs hydrogen peroxide against germs.

[@krishnam4218]

Very good job.

[@deucedeuce1572]

It's amazing that even though the cell is dead, they internals still show signs of life for a while.

[@notallthatbad]

Interesting, and a wee bit like a horror movie. Thanks for showing it!

[@markarca6360]

Even soap does the same thing. Soap dissolves the phospholipid membrane of the bacteria, which are enhanced by mechanical action (rubbing the hands together).

[@FoxWolfWorld]

That wasn’t an oxygen bubble forming it was a small tear in space-time opening a dimensional void

[@beefstew8547]

More than likely

[@diamondkelp3993]

Wonderful thankyou !!

[@chaudry123]

Amazing stuff

[@liveuk]

Stunning what we can see now just for a few quid.

[@airplanegeorge]

those poor little critters

[@aashwood972]

Amazing Oliver. But you did not use dettol. Y ???

[@crowlsyong]

7:45 The way you kindly and gently suggested the subscribe button...so chill. Take my sub and my like and have a great day.

[@marcelmaes5275]

Fascinating indeed. I almost feel sorry for the tiny life. But this microscopic scale is surely a good and quick indicator for environmental pollution and/or climate change.

[@fairdinkum1079]

Can you please do a video with colloidal silver and bacteria

[@spencercoffin4277]

Great video - which microscope / magnification level were you using for this video?

[@____.__._.._]

Its interesting that the rotating behaviour is repetitive. Is this because H2O2 damages cilia, and so there is no directional movement, yet some erratic behaviour remains for some time?

[@julianneedsblood7091]

Beautiful

[@sjp8969]

From 6:30 to 6:38 in the video, What are those faint hairs or crystals forming around the microbe?

[@andrewkamoha4666]

What about sanitary water ???? From low concentration (as in tap water) to high concentrations(as in cleaning products) ????

[@kujaneck]

Thus is sooo cool!!

[@AHIDDENWORLDmicrobiology]

Nice!

[@AmaroqStarwind]

What if you mix alcohol, hydrogen peroxide, salt and soap together?

[@beefstew8547]

Thats what I was thinking

[@astrospeedcuber]

Hi, what magnifying objective was used for these clips?

[@jenniferdarline]

Well this video is 9 months old but if your still reading the comments or someone else knows the answer here is a question. After the cells are exploding you can see long thin spike looking things, are these part of the cell or some sort of crystallization?

[@tauceti.]

Very interesting :-). I wonder how would look like under the microscope the effect of povidone-iodine (alias Betadine) on microorganisms

[@Leradelie]

Thank you for sharing these videos! I have a microscope and have looked at different things, but I haven't known what they were. Looking at a hair with the root attached, there are other things around it, so what are they?

[@simonphuket7782]

How about if you add a drop of kefir? I would love to see what happens if you add beneficial microbes. Are you familiar with the work of Antoine Bechamp and pleimorphism?

[@rommelfcc]

Microbehunter; cool! Did kinda feel sad for their little live... I'm over it now. Can you please test cinnamon. I saw a video when they added cinnamon to their sample and looked pretty effective in killing the microbes. And would be an effective treatment of water when in a pinch? When purifying tablets have run out... And stuck in the worldniss, or post earthquake, and lots of ground water starts flowing out of the roads etc...

[@mr.octopus6972]

Calling a microbe that's dying "a sad thing" really ? 😂

[@XtalProject]

Mr Bean versus Microbes ..... No Contest !

[@israelramos7441]

Will you talk about Thiomargarita magnifica ?

[@dimitriisov1262]

What are the multicolored bits inside the paramecium, especially around 1:06? Are they just other microbes?

[@thedealer799]

I don't know what these guys are called 3:58. I popped one under the 100x condenser using it dry. Where did you collect this sample from?

[@jagibs5494]

MicrobeHunter...can you suggest a good form of hydrogen water capsules for human consumption to rid the internal body of bad micro organisms?

[@PratikChandrani]

Can you please make a video of alkaline water and it's effect on various microbes? Nowadays alkaline water is marketed as miracle water by many companies. I am curious to learn what impact it could have on microbes?

[@Microbehunter]

Depends on the pH of the water. If the pH is 9.5, like some ads claim, it is, then this might already be too high for some microorganisms. As soon as it reaches your stomach, it will be neutralized. There might be additional minerals in them, but this is nothing unusual and independent of the pH. Can try it out.

[@PratikChandrani]

@@Microbehunter Agree, pH 9.5 is most advertised and could be easily tested. It may be neutralised immediately upon reaching the stomach but I think it may still affect the gut microbes, probably adversely. It will be an interesting topic for people, I believe.

[@user-lv9my6jw3x]

​@@MicrobehunterTo add to Pratik, it would be interesting to simulate a scenario where one drinks a glass of water (with different PH levels tested) and mixes with gastric acid (using just HCL for this may be beneficial enough). Using realistic ratios if possible. It would be interesting to see the change in PH of the HCL and the effect it has on microbes. We might infer as to what degree water (and its respective PH) affects the sterilisation and even digestion of an ingested substance.

[@SurrogateActivities]

SPEEEEEEEN

[@tobe3940]

Does Betadine (Povedone Iodine) have the same effect on them too?

[@sioux22]

Vinny would be proud

[@ddbabybella58]

Wow😍 look at those appearance oxygen bubbles look like giant explosion of Nuclear Bombs....Kaboooom...💥

[@pamelarolston9905]

The whole death spin thing is fascinating. I have seen this in some insects as well just prior to death. You can see this spiralling/spin in all of life and even the end of life. The golden mean, Fibonacci sequence, tornadoes (which can bring death) planets and in the study of hyper-dimensional physics ( torsion). Interesting video.

[@Blaiwnez]

Oxygen bubbles look like black holes.

[@TheMoreYouKnoe]

What kind of microscope is being used ?

[@Microbehunter]

I use a compound microscope with DIC contrasting.

[@craigcorson3036]

Are you perhaps from the Schleswig-Holstein area of Germany?

[@LouieG88]

Bleach or detergent!

[@peterc5512]

Hi Oliver, do the organelles, proteins and RNA/DNA, polypeptide and polysaccharide fragments spilled from the cell when the membrane breaks down get also damaged? What would break those apart to beyond recognition by qPCR or other forensic methods of observation? And, does this “soup” in the surrounding watery solution get used up by other cells if this occurs in natural environment and not under the cover glass on a microscope slide which is usually a dead end street anyhow, with or without hydrogen peroxide.

[@Microbehunter]

It is well possible that some proteins denature (change their shape) when the cell breaks up. The proteins are then not supported anymore by eg. a membrane (if they are membrane proteins) or the change in pH also might render then unfunctional. I think this depends mostly on the type of protein and how stable it is. The H2O2 will do a lot of unspecific oxidation and will also break bonds randomly, so I would not be surprised that after intensive H2O2 treatment there would also not be usable nucleotides left for PCR. As a matter of fact, I have used more concentrated H2O2 to completely remove organic material from specimens (I wanted to purify diatom shells this way.) The debris is certainly recycled by other organisms.

[@bendirval3612]

Providone iodine? Chlorhexidine? Antibiotics? Bleach? benzalkonium chloride?

[@Darksagan]

Thats badass.

[@TheOpacue]

Damn cool!,

[@rewschreijewschreier]

i love peroxide.

[@rewschreijewschreier]

it is truly a warrior of goodness. quite visceral. making it's enemies spill their guts all over the place

[@natf5869]

So if they are single cell organisms what is the stuff coming out of them?

[@Haven_city_civilian]

Organelles and proteins.

[@wambee0616]

I was wondering the same

[@pierreracine128]

interesting

[@bilygates2509]

This is encouraging me to do my own research 👍

[@Life_42]

What happens if you jump in a tub of 70%+ isopropyl alcohol holding your breath, eyes closed, nose pinched, and a few seconds later shower in water?

[@ruud9767]

You will start to spin and then explode in a burst of little blobs. A few seconds later the shower will wash everything away.

[@omegaroyal]

smooshy

[@thomasmrkeby6488]

Imagen the oxygen bubble as a black hole and with it there is a giant alien from a different time dimension commenting on the occuring disaster as life dissolves into nothingness.

[@YeetTheAn3On]

hoho