Tissue Culture Propagation: Class 101

2/12/94-Host Steve Dobbs is joined by assistant horticulture professor Doug Needham to help viewers understand tissue culture propagation.

[@theflaca]

Unbelievable! I was working in a hood subbing orchids in 1983. Then to big scaled up industrial size propagation for export, then labs, and genetic modifcation shooting genes into callus with a modified gun. Then virus elimination for quarantine germplasm. Yet after all this I click on this little old vid and I still learn something new. Never seen a roasting bag used for sterile transfer. Never knew it was sterile. thanks for this upload. It's a pearl.

[@thombaz]

What you learned to get in these field of jobs?

[@StaceyHerewegrowagain]

I really enjoyed this video. I'm starting to experiment with tissue culture but I know it's pretty tough to get it going the right way, using different growing media's and nutrient hormones etc.. This is so interesting and I just love the science behind all different walks of nature. Thank you for sharing! I wish I could visit a lab that does this. So fascinating.

[@plantsinjars]

Such a helpful video (even though it was apparently filmed before I was born). I followed your steps in my latest vid :)

[@fortuneflux]

Damn it. I just spent $500 on a small laminar flow hood and I could have done all this in a 20 cent bag..... sigh.....

[@justing8821]

The hood is going to make your life way easier. I use a rubber made container turned on its side at home. My contamination in the rubber made container is about 30 percent. At work I use a flow hood and my contamination is 5 to 10 percent.

[@destinee9903]

Where can you buy that hood for $500?

[@fortuneflux]

@@destinee9903 At the time it was on eBay but I can't any at that price anymore

[@timothygrady7552]

What temps are you storing tissue once inoculated? Incubator or refrigerator? Any gas overlays?

[@carmendompig6804]

Hallo,i want to do this at home,what you use for making de voeding of the plant,i have a lot of flowers to try.can you give me the first step for beginning

[@fatmatunaboylu8122]

Could you show, how do you prepare MS whit hormone for african violet? Which kinds of hormon can ı prefer and how much ml?

[@hoangdang1971]

Where can I get information on large scale tissue culture and freezing plants cells

[@hoangdang1971]

Thank you

[@funny11744]

Could sucrose be replaced by sugar from market ?

[@DeRien8]

Yes, they said table sugar works. Just make sure you don't accidentally use artificial sweetener or syrups. Most of those won't work well or at all.

[@Shibu11229]

At what temperature should the test tubes be stored?

[@cebidexpharmaceutical6314]

26 degrees celcius

[@bonitabromeliads]

What is that high pitched tone in the background?

[@1FISH]

plant screams

[@yurimodin7333]

you see kids back in the day we had VHS.....

[@thombaz]

Would be a great 'scientist' character in a movie.

[@KnobCRICK]

was this filmed in 1998?

[@leadingwithautism]

1994

[@GourmetGaloreNET]

You don't have a laminar flow hood? I've got one of those at my house...

[@russellwilliams7589]

Nerd.

[@GourmetGaloreNET]

@@russellwilliams7589 Thanks :) You caught me.

[@cameronm896]

found the mycologist

[@howaboutnowdad4582]

This looks like a high-school in 1995 dude give him a break

[@KevinSmith-dx6xq]

@@cameronm896 I had to look up what a mycologist did. Thank you, I learned something today.

[@thegautam8240]

Nice 👌

[@jessietanzo6195]

Hi.. where can we buy this materials ang chemical?

[@fortuneflux]

I tried getting the chemicals from Sigma-Aldrich but they won't sell to individuals or home users. So ended going to eBay and Phytotech labs. Also, as he states you can use normal table sugar for sucrose.

[@joekunedo]

@@fortuneflux how did it go?

[@fortuneflux]

@@joekunedo ahh I haven't had opportunity to perform tissue culture yet as I've been waiting for my orchid seed to mature. Orchids require similar procedures, will be able to test in about a month i think.

[@superman200021]

Devonly to break the dormancy period, you should try soaking your plant/seed in a solution of gibberellin.

[@theflaca]

@@fortuneflux I worked a year in an orchid nursery with a lab for flasked stock. We used banana and potato as the main food in the agar. Worked well.

[@Ms2real4ya]

💗🥰💗

[@shesmypresident1637]

Can this be done with dried leaves ?

[@boabab95]

no.

[@fortuneflux]

what he means is "no, because dried leaves are dead tissue. You tissue that is alive and the fresher the better"

[@NYOOOOW]

Where's cartman?

[@LucasGuillemette]

Please don't look in the camera sir. I'd like to keep my soul.

[@plateau2002]

what about his filthy tie dragging all over everything?

[@DeRien8]

Yup, that's the first hint to tell this is just a demo. Once they pulled it out of the bag to see it better and show the cameras it was confirmed. Good point though: clean, tight/well fitting clothing is an overlooked lab standard.

[@theflaca]

And as I've been in the industry of broad scale mass production with a dozen staff chopping away, it was always, hairy arms and beards that produced the most contamination. Even with hexafoam, body hair just carries too many bugs.