Transwell Migration Assay | CSU BMB Exploring Biochem

[@teguhsantoso5248]

Thank you Professor. Very clear explanation for the protocols.

[@deboradasilvabaldivia2277]

Teacher, congratulations on the video, very clear and explanatory. I would like to suggest to you to make a video with Invasion Essay using Matrigel. Thank you very much in advance.

[@lorenademoraisribeirosilva5880]

thank you for sharing this protocol, very educative!

[@eduardoduarte1968]

Thank you so much for sharing this protocol!

[@hudanoor6009]

Well explained 👍

[@petersamuelemeka8710]

Thank you for this video, Professor. You said "clean upper membrane to remove migrated cells", then "fix the cells on lower membrane". How many membranes has the insert?

[@coagulatedsalts4711]

there is one membrane. She is referring to the top of the membrane and the bottom. The top has cells that can migrate to the bottom. When she cleans the upper membrane (the top of the membrane), she is removing cells that haven't migrated out of the top well, at the lower membrane (the bottom of the membrane) there will usually be cells that have migrated if the treatment is unsuccessful. if the treatment is successful, no cells will migrate at all! hope that helps.

[@LionLion632]

Thanks so much for clear explain. Which staining dye did you use ?

[@MedguidewithDr.Mudasirsaeed]

Thankyou it was very informative video 🌹

[@himanshusarkar9412]

Thankyou it was very nice video 😊

[@sci_djw7670]

We have this assay working well in our lab with automated quantification of migrated cells 😊

[@algirmaalkhamkhuu3223]

Could you please share your protocol or methodology?

[@avocadosnumber498]

Thanks :)