Migration Assay

( www.abnova.com ) - The migration assay (also known as the Boyden Chamber Assay) is a commonly used test to study the migratory response of endothelial cells. During this assay, cells are placed on the upper layer of a chamber and a solution containing the test agent is placed below the chamber. Following an incubation period, the cells migrated through the membrane are stained and counted. More videos at Abnova www.abnova.com

[@muthiikawati8306]

I have never been using this method, but as far I know Boyden chamber has a porous membrane at the bottom where migrated cells "trapped", scrapping only remove non-migrated cells. cmiiw

[@asmaaamel1840]

you wash with PBS+ or PBS - ? how much volume of giemsa, methanol and formaldhyde is added ? 3,7 % of formaldhyde in PBS- or PBS + ? it should be noted

[@yannacademy]

Thanks for the video. I especially appreciate the comment that follow the video indicating the layer that contain the test agent. Thanks a bunch

[@rippeduthjghgkj9]

did anyone count?

[@Throughmylens01]

what is the catalog number of the migration chamber?

[@kunalapavan]

Thanks for sharing. It will be very helpful for me while performing the experiment.

[@francescosilvestro2092]

Dear Abnova, where could I found the music in the background? Are there any kinds of playlist?

[@Learningnfun643]

After swabing with cotton how do you know which are migrated cells??why cotton swapping done???plz assist

[@deborahwokpetah6600]

THIS VIDEO HELPED A TON!! THANK YOU

[@20rayt]

It would be good if you cut the membrane and then mount it on a slide and observe/count cells. Thanks

[@calummcfetridge7649]

Most protocols I've seen suggest scraping off non-migrated cells prior to fixation and staining. However I can see the appeal of performing this step after staining, as you can see if the apical stained cells have been removed. Does anyone know if either method is superior?

[@takina9445]

Do you mean scratch by scraping?

[@abdullahimohamedsaid1813]

is it same procedure when we use crystal violet die? this experiment is used Giemsa stain instead of crystal violet.

[@learn-bioinformatics]

AWESOME!! It really helped me a lot!!

[@iraklismoschonas5214]

Do you know how I can count the migrated distance of the cells?

[@hamadali3400]

Iraklis Moschonas I think some researches using image J software

[@prayaschakmashanto5275]

How much pore size of the insert?

[@manuelp.jimenezgarcia6703]

Does anyone have the exact reference for Abnova's boyden chamber product?

[@ShanatSD]

Thanks for the video, it was very very helpful!

[@mitalbhatt5558]

how to cite this video tutorial

[@user-pv3ui5uj3f]

Thanks!

[@m7mady0]

great video

[@judnarokjudnaddff6541]

excellent vidio.

[@pallekranthi]

excellent