Do you have a video where you show how to count cells in an invasion assay? Like how to compare surface cells to invaded cells and how to tell them apart?
@AllThatExists_Channel12 жыл бұрын
i am gonna do this experiment....it's really helpful for me....thanks so much
@aditibanerjee21923 жыл бұрын
What is the dilution of Matrigel? I could see the same question asked by MG.
@burcuyener74212 жыл бұрын
Do you touch to the membrane when you scraping off with cotton swaps?
@hothiluu55562 жыл бұрын
Why do you count invasive cells in the upper chamber? It should be counted in the lower chamber, right?
@emisaelstenio92544 жыл бұрын
congratulations on the work, I would like to know which matrigel was used for the experiment and which was the dilution of the matrigel
@clydevan77573 жыл бұрын
instablaster.
@anil31616 жыл бұрын
What is that lower chamber? Can v see tube formation using this chamber? I mean angiogenesis assay
@歐彥廷-n5e4 жыл бұрын
what is matrigel dilute ratio?
@ratihsuprapto53694 жыл бұрын
How much % of giemsa that used in this video ? thank you so much
@rosemania998 жыл бұрын
Excuse me, but what's the difference between this assay and the migration assay? I see they're the same except for the Matrigel step here.. Another question; what is the function of the matrigel in this assay? Thanks.
@rraff79386 жыл бұрын
Matrigel is what the cells migrate through. It mimics a basement membrane. The migration assay measures migration across a surface- a pipette is usually used to clear an area of cells. The Invasion assays measure cell movement through a medium (matrigel on a filter insert).
@isabelgomez18524 ай бұрын
Matrigel and time are two factors that differs between migration and invasion. You don't need matrigel for migration essay, and you can evaluate it at 12 or 13 hours. Invasion needs matrigel because it mimics ECM with BM elements, just as an invasive cell. And it takes around 22 to 24 hours.
@JoiDior9 жыл бұрын
The BD protocol specifies to removed the membrane from the insert with a sharp scape. Does anyone use this technique before counting the invaded cells?
@edward8208289 жыл бұрын
Wash the chamber by PBS before fixing cells... Won't the cells on the membrane be washed out ?
@黃湘萍-v3g9 жыл бұрын
每天面對這些無趣的東西,會不會打瞌睡?
@edward8208289 жыл бұрын
哈哈…不會無趣啦
@黃湘萍-v3g9 жыл бұрын
了不起! 祝你成功!
@heycharliemot6 жыл бұрын
Nope, because these are adherent cells so they stick to the chemically treated plastic chamber . We also use PBS to wash the debris off culture flasks when changing complete media.
@ddy001636 жыл бұрын
then you mean if I use suspension cells for same experiment, then cells will be washed out? then what should I have to do with suspension cells?...
@Jason-o5s5 ай бұрын
Cheer~~~determine the content or quality of (a metal or ore).