Vesicle Cycle Explained (& Clathrin-Mediated Endocytosis) | Clip

Welcome to Science With Tal!
In this video, we will cover the main steps of the vesicle cycle. The video will cover neurotransmitter uptake, the reserve pool, docking and priming to membrane, exocytosis and endocytosis. The video will also cover the kiss and run and kiss and stay pathways.
To improve the quality of my content, I highly value the feedback from the viewer so do not hesitate to give any feedback in the comment section.
Timestamps
0:00 Introduction
0:16 Brief historical perspective
0:52 Neurotransmitter uptake
1:41 Reserve pool
2:06 Docking & priming to membrane
3:43 Fusion (exocytosis)
5:00 Complex disassembly (SNARE dissociation)
5:38 Clathrin-mediated endocytosis
6:33 Reversible fusion pore mechanisms (Kiss and run/stay)
7:40 Summary of each step and each protein involved
8:16 Conclusion
Resources used
Here is a list of the resources that I’ve used to produce this video. (Author: title resource)
- Dale Purves: Neuroscience (6th edition)
- Eric Kandel: Principles of neural science (6th edition)
- Robertson, J. David: Some Features of the Ultrastructure of Reptilian
Skeletal Muscle
- Heuser, J E et al. : Synaptic vesicle exocytosis captured by quick freezing
and correlated with quantal transmitter release
- Heuser, J E and T S Reese: Evidence for recycling of synaptic vesicle
membrane during transmitter release at the frog neuromuscular junction
- Llinás, R and Y Yarom: Properties and distribution of ionic
conductances generating electroresponsiveness of mammalian inferior olivary neurones in vitro
- Katz, B, and R Miledi: A study of synaptic transmission in the
absence of nerve impulses
- BOYD, I A,and A R MARTIN: The end-plate potential in mammalian
muscle
- DEL CASTILLO, J, and B KATZ: Quantal components of the end-plate potential
To have more information on these resources, you can refer to the conclusion section where a more formal citation is provided.
Video credits
Writing: Tal Klimenko
Voice: Tal Klimenko
Animations: Tal Klimenko
Drawings: Tal Klimenko
Editing: Tal Klimenko
Introductory jingle: Thierry Du Sablond
Conclusion music: lukrembo - jay ( • lukrembo - jay (royalt... )

[@silviaaaaaa]

There seems to be a mistake at 1:37, where the vGLUT anti porter is shown: instead of ACh import, there probably should be written GLUT import no?

[@sciencewithtal]

You are right, it should say glutamate import and not acetylcholine. Thank you for pointing that out!

[@christianvandermaas8932]

Your videos have been a real treat to enjoy.

[@sciencewithtal]

Thank you!

[@That_Chemist]

This is helpful context for our upcoming neurotransmitter video! Many thanks!

[@sciencewithtal]

Thank you for the feedback, I am glad it was helpful!

[@palomabrt]

Those videos are amazing, well done for your work (animation + content). I'm honestly so surprised that you only got a hundred followers, you deserved thousands !!

[@sciencewithtal]

Thank you for your support!

[@donut_eeba]

I JUST FELL IN LOVE❤❤❤❤❤

[@johnb7762]

What a god send. Thank you Tal!

[@sciencewithtal]

My pleasure!

[@thesecularfactman9307]

Impeccable . Thank you🙏

[@sciencewithtal]

Thank you for the feedback!!

[@marisolceja]

Thank you so much for this video! Super helpful!

[@sciencewithtal]

Thank you for the feedback!

[@griseldapadilla8747]

Impeccable!! Thank you 🙏

[@sciencewithtal]

My pleasure!

[@Abdo-vg1ni6mz2v]

Thank you very much for this professional videos 🎉🎉❤❤

[@sciencewithtal]

My pleasure, thank you!!

[@charlientumba6673]

Thank you so much 🙏🏾

[@sciencewithtal]

Thanks for the support!

[@futuredocteur]

Thnk u so much this video is so helpful but I have a question what is the role of zippering??

[@sciencewithtal]

I am glad you found the video useful! Could you clarify what you mean by 'zippering'?

[@futuredocteur]

@@sciencewithtal I found it in a scheme where it was used as something used to push the vesicle into the membrane but I'm not sure because it wasn't so clear

[@sciencewithtal]

@@futuredocteur From some diagrams I have seen online, is it possible that zippering refers to the association of the SNAREs? That would make sense, given the fact that SNAREs are an integral component of exocytosis (pushing vesicles into the membrane). Let me know if that makes sense!

[@futuredocteur]

Can I contact you in social media? In order to send you the diagramme ?

[@sciencewithtal]

@@futuredocteur Yes of course. Send it to me at [e-mail],and I'll take a closer look!