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@hifza21074 ай бұрын
What is Sanger sequencing? A conventional method used since 1977, is still used today In this we denature the DNA, by increasing the temperature. Cool down to cause primers to anneal. DNA polymerase then extend the DNA fragments until a fluorescently labeled dideoxyribonucleotide is reached. The chain is terminated. The colour of the florescent indicates the base at which the chain is terminated. When the DNA fragments are separated by size, we can determine the sequence of terminal nucleotides and determine the sequence of DNA/genome
@leenahmed147 Жыл бұрын
Spectacular explanation! It’s amazing how you simplified it thank you
@AliShaikh_7449 ай бұрын
Very concise yet gets the concept across in an excellent manner which is very easy to understand! Thank you!
@beckseamons Жыл бұрын
Wow. Amazing explanation, it really helped me understand how genius this method is.
@EllieBrowne-z1i7 ай бұрын
Great video! One thing--I think it's misleading to say the ddNTP is "randomly" inserted. It's inserted according to Watson-Crick rules.
@poldi77 ай бұрын
He means that, by chance, a ddNTP is inserted instead of a normal dNTP, which would not stop elongation.
@EllieBrowne-z1i7 ай бұрын
Yep, I get that! Just pointing out that his statement could be misinterpreted. @@poldi7
@poldi77 ай бұрын
@@EllieBrowne-z1i Yeah, that could be true. You‘re right
@doanhatvyАй бұрын
Lovely and simple to understand!
@ReseekaN2 ай бұрын
superb explanation
@ZARIASALIM Жыл бұрын
Great explanation
@wahhajmustafa1907Ай бұрын
Thanks a lot! This was easy to understand
@greennamm19079 ай бұрын
Thank you! You're a lifesaver!
@Kai-dl9cp11 сағат бұрын
does anyone know the name of the background music?
@erzascarlet47Ай бұрын
Wait.. isn't this the procedure for Polymerase Chain reaction?
@rockyisaac5612 күн бұрын
Omg Wow i totally get it now..
@egegezer82205 ай бұрын
Finally a good video
@rabia40098 ай бұрын
I don't understand how we get to know about the sequence and why we are terminating it with ddNTPs?? Can someone help me with this?
@barbarosxeczaci74106 ай бұрын
When we terminate the chain with ddNTP’s we understand that in this spesific place there is spesific nucleotide that we added in the tube and after that you repeat the action and figure out all of different spesific places too. I hope i could explain you
@zemaxe13 Жыл бұрын
@0:48 ... is missing a hydroxyl group which allows the next nucleotide to bind - you mean it does not allow the next nucleotide to bind, thus ending in termination...
@promega Жыл бұрын
Hi there, thanks for your comment. The dideoxynucleotide is what terminates the chain.
@CoalCrafteris11 ай бұрын
Hydroxyl group allows the next nucleotide to bind, thus ddNTP (which is missing one) terminates the sequence, he explained it fine.
@aditisoumendra11507 ай бұрын
Yeah, basically we need a hydroxyl group at 3' carbon atom in a pentose, for phosphodiester bond to be formed and for DNA sequencing to happen, right.. so ddNTP lacks that 3' OH group as well. So no further bond formation, and no more sequencing.