Why you shouldn't routinely use antibiotics in mammalian cell culture, & when you should use them

[@healthbabe2691]

That is resourceful. Thanks for sharing your knowledge again along with the links for the related scientific literatures.

[@GeorgeCelis-en8jc]

Your videos are awesome, I’m starting my biochem PhD in September and your videos just build up my excitement! 😊

[@thebumblingbiochemist]

Thanks so much! And how exciting! Best of luck with your studies and reach out if anything comes up you have questions about.

[@thebumblingbiochemist]

For more information: * Hassan, S. N., & Ahmad, F. (2020). The relevance of antibiotic supplements in mammalian cell cultures: Towards a paradigm shift. Gülhane Tip Dergisi, 62(4), 224-230. doi:doi.org/10.4274/gulhane.galenos.2020.871 * Ryu, A.H., Eckalbar, W.L., Kreimer, A. et al. Use antibiotics in cell culture with caution: genome-wide identification of antibiotic-induced changes in gene expression and regulation. Sci Rep 7, 7533 (2017). doi.org/10.1038/s41598-017-07757-w * Eppendorf, Contamination in Cell Culture, handling-solutions.eppendorf.com/cell-handling/contamination/introduction/ * addgene, Tetracycline (Tet) Inducible Expression www.addgene.org/collections/tetracycline/ More on antibiotics as selectable markers: blog form: bit.ly/antibioticselections ; KZbin: kzbin.info/www/bejne/mna6qKSleradobs   More on antimicrobial resistance: blog form: bit.ly/antibiotic_resistance_mech  ; KZbin: kzbin.info/www/bejne/q5azkGyMn5KEZs0 more on mammalian cell culture media: bit.ly/cell_culture_media & kzbin.info/www/bejne/bniuf2lmf56geZY More posts on mammalian cell culture: bit.ly/cell_culture   More videos on mammalian cell culture: kzbin.info/aero/PLUWsCDtjESrH_qWp2oWcVMH9DLlZreX92   more about all sorts of things: #365DaysOfScience All (with topics listed) 👉 bit.ly/2OllAB0 or search blog: thebumblingbiochemist.com

[@natalias374]

Just in time as I’ve started a course in cell cultures at my uni

[@thebumblingbiochemist]

Best wishes with the course! Hope you enjoy