CRISPR Cas9 vs. Cpf1: 5 ways the Cpf1 nuclease outsnips Cas9

What's the difference between CRISPR Cas9 and Cpf1?
➜ Dear fellow biotech lovers, thanks for watching our first episode of Molecular Minutes! In this episode, we introduce you to Cpf1, a new CRISPR nuclease discovered by the Zhang lab which we'll soon make available over here at abm. Cpf1 is smaller than Cas9 and targets complementary areas of the genome, making it an excellent addition to the CRISPR dream team. Watch our video to learn more about why Cpf1 deserves its own fan following!
For more info on the CRISPR Cpf1 or Cas9 system and for a list of the sources used, please visit:
➜ Our Knowledge Base: info.abmgood.c...
➜ All CRISPR Resources: info.abmgood.c...
Want more tips, tricks, guides, and protocols on how to knockout a gene using CRISPR? Join our free, 4-week #CRISPRCrashCourse:
➜ info.abmgood.co...
*Note: For those of you who may have turned off annotations, please be aware of the error at ~1:32 mins: trans-activating CRISPR RNA is abbreviated as "tracrRNA" not "tRNA". Our apologies!
Check out our other video series:
➜ Polymerase Chain Reaction (PCR) - An Introduction: • 1) PCR (Polymerase Cha...
➜ CRISPR Cas9: • CRISPR Cas9 - A Brief ...
➜ Adeno Associated Virus: • 1) Adeno Associated Vi...
➜ Cell Culture: • Cell Culture
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[@anonymoushy7815]

I do think that Cpf1 has a lot of potential because of its sticky end cuts and that it doesn't need cRNA. However, because Cpf1 is pretty new, while Cas9 has already been around for years, so perhaps people don't want to use Cpf1 over Cas9 for now since that would mean a lot more research and trials. But who knows, I hope Cpf1 gets more interest in future.

[@abmgood]

Thanks for your comment!

[@JorgeRamos-xw6dy]

CRISPR Cas9 is in many late stage studiesand if and when approved their technology will get an immense boost. There will always be newcomers but the true and tried can not be easily swept aside.

[@chitraanbu6430]

Tq so much for this informative video

[@user-nb9dt2lo8i]

Thx for providing this great video!!!!!

[@abmgood]

No problem! ;) Glad you found the video helpful.

[@AdolfsTimrots]

Good video with a great potential. It would be nice if you got better microphone. Keep it up!

[@jumpingpools]

Love the dad jokes. Thanks for the info, it's been helpful!

[@rakibalmahdi2401]

can somebody explain between T-rich PAM sequences and G-rich PAM sequences?

[@abmgood]

Hi Rakib, thanks for watching and commenting -- Cpf1 is considered to have a "T-rich" PAM sequence as it's PAM sequence is "TTN" whereas Cas9 is considered to have a "G-rich" PAM sequence as its PAM sequence is "NGG"! Hope this helps!

[@ManosTheOne]

Hi Rakib. I have a better explanation. T-rich PAM sequence is T-rich and G-rich PAM sequence is G-rich. Hope this helps!

[@jiji-f5j]

good video i can understand the cpf1 more thank you

[@salarahmad1039]

wow brilliant thanks mam please give me any good advice about study i would be thankful to you for that.

[@mikebenstead7600]

Who, if anyone, monitors and regulates CRISPR usage? What's to stop this tech falling into the wrong hands?

[@rakeshhrx617]

Hello I'm rakesh from India , I'm a muscular dystrophy paitient, can you say me whene it's start in human body ???

[@abmgood]

Hello Rakesh, This disease is genetic based and is related to many factors - it would be best if you talk to your doctor for more information!

[@rakeshhrx617]

Applied Biological Materials - abm You are right but Indian dr are so lazy he knows about this tretment but not refare any kinds of tretment. He says nothing any kinds of tretment right now just wait and watch your dade time.