He looks like dr mandell but with hair HAHAHAHAHAHAHAH
@ivapadourkova86309 сағат бұрын
Greetings from the Czech Republic and thank you very much for your videos, which are very inspiring for me, you explain everything beautifully. Today I start eating according to 18:6, so we'll see when I lose a few kg. Beautiful days :-)
@MikeCola7 сағат бұрын
Thanks so much and good luck with 18:6 👍
@abhi_ya_kabhi_nahitelegram452111 сағат бұрын
I have been following OMAD diet since 3 months, Now I'm feeling very good and energetic. But my blood pressure is 65/100. Do I need to worry?
@givetothepoor5523Күн бұрын
I do the 36 hour fast 3 times a week with no food on my fasting days. I was surprised at how i wasnt as hungry as i thought i would be on my eating days. This is my 3rd week and im adjusted to it. Headache at the beginning but its smooth sailing now! Keep pressing 💪
@pranavpendse8611Күн бұрын
I am following OMAD everyday and i will come here after 3 months ie 18 th November 2024
@pixielated2003Күн бұрын
I have had no gallbladder for 10 years and suffer all the time with weight gain,sickness ,diarrhea, and other issues. Doctors do not recognise or don't want to take on that they have not told you this before they remove the gallbladder or the fact that the ibs type symptoms or because of this . I recently had a health check and was told not to eat all the things I don't eat . I'm sure they don't believe me . I'm now working on an 18 hr fast as a last resort to loose weight. As they say I am high in cholesterol and insulin pre diabetic They have never after many visits had an answer . Your video is the most informative simple guide and reassurance that I have needed on my journey forward . I know I'm not alone with these issues as there are so many confusing long winded videos. I want to thank you so much for your practical approach and explanation .
@charlesacaranci9039Күн бұрын
Who the hell would want to train this way if a normal human being
@chemurrКүн бұрын
Coach
@MikeColaКүн бұрын
You can follow this link and fill out an application to learn more about my coaching program: mikecola.typeform.com/to/xOjnQtp2 Best - Mike
@decrypt832 күн бұрын
1. 30- 40 min. brisk walking and 10 sit-ups a day 2. Adjust what you eat 3. Adjust how much you eat 4. Be happier because you are not just eating diet
@sasharumbold40042 күн бұрын
Loving your videos and contents, thanks soo much Mike! Have been vegan for 12 years. My diet is basically all carbs, after watching some of your videos I understand now why I need to up my protein but I think may have to supplement as can’t see me reaching protein requirements through food alone, any advice? You’re super, thanks!
@MikeCola2 күн бұрын
Thanks so much.. Try increasing your protein I think it would be a good idea. my brother is a vegetarian and my best friend is vegan they both increase their protein and definitely felt a difference, muscle mass and strength.
@sasharumbold400410 сағат бұрын
@@MikeCola thanks for the advice😊 I had a terrible ankle injury two years ago and was on crutches for 11months, it’s still not quite right, have struggled regaining muscle.. have ordered some hemp powder and will make mindful decisions to up protein through food also 🤞🏻
@leowvm62152 күн бұрын
👍🏿👍🏿👍🏿
@MikeCola2 күн бұрын
🙌🙌
@Stefano-gr4xo2 күн бұрын
Good video Thank You Mike.
@MikeCola2 күн бұрын
Glad you enjoyed it
@GallivantingGrandma2 күн бұрын
How is autophagy measured?
@MikeColaКүн бұрын
Autophagy is a cellular process that involves the degradation and recycling of cellular components. Measuring autophagy can be complex because it involves multiple steps and can vary across different cell types and conditions. Here are some common methods used to measure autophagy: 1. **LC3 Conversion and Detection:** - **LC3-I to LC3-II Conversion:** LC3 (microtubule-associated protein 1A/1B-light chain 3) is a key protein involved in autophagy. During autophagy, LC3-I is converted to LC3-II, which is associated with the autophagosome membrane. This conversion can be detected using Western blot analysis. An increase in the LC3-II/LC3-I ratio is often used as an indicator of autophagy. - **Immunofluorescence Microscopy:** The localization of LC3 can be visualized using fluorescence microscopy. The formation of LC3-positive puncta (small dots in the cytoplasm) indicates the presence of autophagosomes, which can be quantified to measure autophagy levels. 2. **p62/SQSTM1 Degradation:** - p62/SQSTM1 is a protein that binds to ubiquitinated proteins and is selectively degraded by autophagy. A decrease in p62 levels, detected by Western blot or immunofluorescence, is often used as a marker of increased autophagy. 3. **Autophagosome and Autolysosome Counting:** - **Electron Microscopy:** This is a gold-standard method for directly visualizing autophagosomes and autolysosomes at the ultrastructural level. Counting these structures provides a direct measure of autophagy. - **Fluorescence and Confocal Microscopy:** Cells can be stained with fluorescent markers specific to autophagosomes and lysosomes. The colocalization of these markers can be analyzed to measure autophagic flux. 4. **Lysosomal Degradation Activity:** - The degradation activity of lysosomes can be measured using substrates that release a fluorescent signal upon cleavage by lysosomal enzymes. An increase in lysosomal degradation activity can be indicative of autophagic flux. 5. **Autophagic Flux Assays:** - Autophagic flux refers to the complete process of autophagy, including the formation of autophagosomes, their fusion with lysosomes, and the degradation of cargo. To measure flux, inhibitors like bafilomycin A1 (which blocks the fusion of autophagosomes with lysosomes) are used in combination with the detection methods above. By comparing conditions with and without the inhibitor, researchers can determine the rate of autophagic flux. 6. **GFP-LC3 or mCherry-GFP-LC3 Reporters:** - Transfection of cells with GFP-LC3 or mCherry-GFP-LC3 constructs allows for real-time monitoring of autophagy. GFP-LC3 transitions from a diffuse pattern to punctate structures as autophagosomes form. The mCherry-GFP-LC3 tandem fluorescent reporter allows for the distinction between autophagosomes and autolysosomes, as the GFP signal is quenched in the acidic lysosomal environment, while the mCherry signal remains. Each method has its advantages and limitations, and often, multiple approaches are used together to get a comprehensive understanding of autophagy in a given system.
@chadjohnson43272 күн бұрын
How much arugula (2 cups)
@MikeCola2 күн бұрын
About 100 grams
@chadjohnson43272 күн бұрын
@MikeCola ah so you measure your food on a scale
@ibtissemgarram48863 сағат бұрын
@@MikeCola too much!
@MikeCola52 минут бұрын
The container that I buy it it is 142 g so I just take 2/3
@markzirpolo99342 күн бұрын
Wheres the wine lol
@MikeCola2 күн бұрын
A little wine now and then 🍷🙌
@userpedroo2 күн бұрын
Jesus Loves You
@userpedroo2 күн бұрын
Jesus Loves You
@userpedroo2 күн бұрын
Everything is going to get better
@userpedroo2 күн бұрын
Thank You 🌅🦁
@userpedroo2 күн бұрын
Jesus Loves You follow Him ❤️💔🙏📖🪔
@fizz13-yf8yk3 күн бұрын
Yeah if you are single...number 10 is no win ..I don't trust anyone to be that close to them....
@dcwoods1003 күн бұрын
I always have a glass of wine with my dinner in my feasting 4 hours, still loose weight
@dcwoods1003 күн бұрын
I do 20/4 fasting usually 5 days a week, on weekends I usually have breakfast and sometimes lunch if I want, is that ok?
@justineduplessis61823 күн бұрын
Thank you so much for this video because almost i give up 😂 i am fasting 36 hours one's a week now i drop 1kg next day i pick up 1.5 i woke every day i eating less whats going on
@Alfonso5054 күн бұрын
Have sex regularly 😢
@Okoye19644 күн бұрын
Worst part of it all... STILL GONNA DIE!
@leowvm62155 күн бұрын
👍🏿👍🏿👍🏿
@MikeCola4 күн бұрын
Thanks 🙌
@iriseiselt9345 күн бұрын
Best video ever 🥰🥰🥰👏 Thanks 🙏 I have since ages 105 kilos 🥲🥲🥲 Goes up and down 2 kilos & stays there 🥲🥲🥲 Maybe I should try to drink only 3 shakes per day for 3 day’s in a row 😅😅😅✅
@notjustaprettyface5 күн бұрын
Best delivery of information on this topic! Much appreciated sir ❤I thought I knew everything it was to know about fasting until watching you.
@MikeCola4 күн бұрын
Glad it was helpful!
@notjustaprettyface4 күн бұрын
@@MikeCola very!
@iriseiselt9345 күн бұрын
Thanks for your great advice 🙏🙏🙏🥰
@MikeCola4 күн бұрын
You are so welcome
@s.d.72195 күн бұрын
Looking amazing! The music and the message is great Mike!
@MikeCola4 күн бұрын
Much appreciated!
@nicoleokal12705 күн бұрын
When intermittent fasting, is it ok to eat the carbs with the first meal of the day (at 12) and then don't eat any carbs at dinner? I am at my hungriest at lunch time so I feel that this would be best for me/
@MikeCola4 күн бұрын
Yes, that can work 👍
@lindagomez21335 күн бұрын
You are so lucky, I have problems sleeping after my husband died 9 months ago . But with your lessons I have lost 6 kilos in a 5 months, thank you 😊
@MikeCola4 күн бұрын
So sorry about your husband. I'm glad you're finding my videos helpful and you're losing weight 👍 stay with it 🙌
@ej69815 күн бұрын
Great advice. Thanks Mike.
@MikeCola5 күн бұрын
Glad it was helpful!
@Footy885 күн бұрын
I can do everything other than 10th 😅
@barbberg88336 күн бұрын
Mike, have you dealt with people prima Morray women, that are older like myself and have hypotension? It’s like impossible to lose.
@barbberg88336 күн бұрын
Love you & your message but hate that music
@MikeCola6 күн бұрын
Thanks so much. I will, change the music. Thanks for the tip.
@7727ish5 күн бұрын
Maybe not the music but he needs to change?
@iriseiselt9346 күн бұрын
Great 😊 advice thanks 🥰🥰🥰💖
@MikeCola6 күн бұрын
You are so welcome
@vondalittle94256 күн бұрын
Looking good Mike thanks for the info
@MikeCola6 күн бұрын
Thanks 👍
@baharv45756 күн бұрын
I’m trying to get my stomach down to where I’m shredded
@MikeCola6 күн бұрын
You can do it 🙌
@vibekes24166 күн бұрын
I have to take cortisone every day, because of a transplantation I had in January. But I still have hope to loose weight
@FormlessJKD176 күн бұрын
So it's not 36hrs fssting if you take in 500 cal😂
@adelsonlorenzo3816 күн бұрын
I lost 10 pounds in a month
@MikeCola6 күн бұрын
Nice 🙌
@gaidarskimsn7 күн бұрын
Thank you!
@MikeCola6 күн бұрын
You're welcome!
@evankamall94287 күн бұрын
I'm currently doing the 20 hr fasting everday is it bad for my body ? Also i do workout everyday please answer me sir
@MikeCola6 күн бұрын
As long as you're taking enough protein nutrition, I think it'll be OK... if you start getting weaker in the gym, then I would take a break
@susu84467 күн бұрын
I do 22 hours a day and the first thing I do. I the morning is eat lol
@ShapochkinKirill7 күн бұрын
Thanks for the podcast Mike! (It ended just after I wrote those words 😢).
@ShapochkinKirill7 күн бұрын
P.S olive oil, nuts , dark chocolate calories may add up... healthy yes, calorie dense also yes.
@MikeCola6 күн бұрын
Sorry, Kirill, we had technical difficulties... the picture and sound was going in and out. I'll repeat this livestream
@ShapochkinKirill6 күн бұрын
@@MikeCola I understand, don't worry!
@ruortega71257 күн бұрын
Always love your content Mike. 1 Avocado a day!!!! - Ru
@MikeCola6 күн бұрын
Thanks so much 😄
@andrewaarons50587 күн бұрын
i'm able to have a 2000 calorie meal in one sitting it's possible if you wait till you're well hungry like about midday and that's working for me prostate is better than it's been in many years because inflammation is reduced body is functioning better arthritis is reduced I can walk as long as I want I can do as many squats as I want I'm 64 years old I'm at the chin up bar did 25 today not all at once mind you and I don't feel hungry after having a big protein and nutrient dense meal 80 g of protein. The typical meal I eat contains eggs pork products pancakes potatoes sliced tomato And the rye toast ,a liver meal twice a week , Black coffee for breakfast if you can call that breakfast I'm doing good thank God ,I hope this helps somebody.
@mehdibenseghir6638 күн бұрын
I think bro forgot to add some protein to his meals