Kinetochore | Structure and Function | Molecular biology

[@ch.k4580]

A really nice quick summary. Maybe some things I want to mention. You can call it inner and outer kinetochore but to be correct you have to call the inner kinetochore CCAN (constitutive centromere associated network) and outer kinetochore KMN (KNL1-Mis12-Ndc80 complex). At minute 2:33 you only mentioned a few of the about 100 proteins involved. Moreover, you mixed the KMN with the fibrous corona and the microtubule associated proteins. Its is important to distinguish. To fully understand the kinetochore and its composition it would have been very helpful to show how the building blocks are from the centromere to the actuall KMN network. Also here you could have added a short description how the kinetochore has components which are evolutionary conserved and other not. Moreover, some of the organisms are not yet know to have one (see Drosophila melanogaster). There was even a recent structural breackthrough published in Science, where the structure of the human CCAN upon binding to alpha-satellite containing CENP-A Nucleosomes (Group of David Barford in England) looks like. That would have been a really nice addition to your depiction. Moreover, you were talking about the destruction of the cohesive rings. One thing you did not mention was, that phosphorylation events on the kinetochore and centromere associated proteins play a vital role in this process too. Different phosphorylation states actually determine if the cell now can divide the sister chromatids when the SAC (spindle assembly checkpoint) as well as the CPC (Centromere passenger complex) "agree" on the right attachment and tension of the spindle microtubules to the centromere. Other kinases (CDKs, PLK1, AuroraB etc.) also play an important part in this. I hope you find this information useful. I wish you all the best and keep on going!

[@animatedbiologywitharpan]

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[@waqarhasan1009]

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[@animatedbiologywitharpan]

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[@microbioman8164]

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[@animatedbiologywitharpan]

thanks for your feedback

[@getreadywithme785]

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[@animatedbiologywitharpan]

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[@animatedbiologywitharpan]

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[@ramchandrasuthar2848]

GREAT ANALYSIS 👍

[@advancedsubsidiarylevel2-qy6uk]

not clear pronunciation