Salting in and salting out | How does salting out happen? | what is salting in of proteins?

This video explains the concept behind salting in and salting out in details but within 5 minutes. this video will answer following questions
What is meant by salting out?
How does salting out happen?
What is salting out in protein separation?

[@ranjitshaw9110]

This is the best video for explaining the concept of Salting in and Salting out.... Thankyou dada for this....l would like to ask you about the reference where l can get the exact text of this explanation!!!!!

[@animatedbiologywitharpan]

There is no 1 proper source for it ....several review articles and text books . But you can follow voet and voet

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@ranjitshaw9110]

@@animatedbiologywitharpan Ok dada....l will try to look into them

[@ranjitshaw9110]

@@animatedbiologywitharpan Sure

[@ranjitshaw9110]

Is there any video of Parkinson's disease made by you?

[@shawnmakiri4435]

Thank you. You did something my textbook and my lecturer couldn't do.

[@skydivenext]

no is because is very boring why do I have to study stupid biochemistry for my degre what a joke bro lol

[@martaromeromarcos5816]

our teacher's explaining his subject with ur video, you can be proud bro

[@animatedbiologywitharpan]

Marta Romero Marcos please share my channel link with your friends and help me to reach big audience

[@marcosmartinandres710]

@@animatedbiologywitharpan She shared it with me!!! I've learnt a lot!

[@gigiarias7659]

Such a great refresher a year after my biochemistry courses! The graphics are really helpful, great work!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@jenniejean-baptiste9467]

I usually have a hard time understanding how some of these processes work but you did an amazing job at explaining the process! I feel like I understand it and could explain how it works to another individual now. Thank you!

[@animatedbiologywitharpan]

In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

[@pawanrohilla01]

You are a ray of hope for all of us aspirants like me 🇮🇳🇮🇳

[@pieromg8587]

Thank you so much for making this fantastic video ❤

[@animatedbiologywitharpan]

Please support me by subscribing to mu second channel youtube.com/@letscrackbioexams94?si=l2Di6Uc4hCYYa3ve

[@millykunda4537]

You are legit making biochemistry easy for me 🙌

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@SuperPuddingFish]

Short and clear, thank you!

[@ria7657]

finally it's crystal clear to me...thank you so much!

[@animatedbiologywitharpan]

please share among your friends

[@danahhamad8088]

I was struggling with this concept but you sir explained it very well. Thank you 🙏🏻

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@rayaroy519]

Thank you for this lecture video. I have never understood this topic before and the mechanism behind it. This video has done it for me.

[@animatedbiologywitharpan]

Do explore other videos I. My channel.... if you like my content please share among your friends

[@megnareddy9499]

Thank you!!! This video was very well explained and helped me understand something even my professor could not help with

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@asylahblairewahing198]

Thank you for this. Was able to understand it better thanks to this video.

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance namaste 🙏

[@strikefreedom7575]

Helps a lot! Thanks!

[@animatedbiologywitharpan]

Please share among your friends and help me to reach big audience

[@argowal]

Great work, thank you for your presentation, it was clear and informative. the use of graphics greatly helps, keep it up! Would love more about protein separation and electric properties.

[@animatedbiologywitharpan]

Please share among your friends and help me to reach big audience

[@조하성-p2d]

Best explanation for salting in and out. I want to cite this reference Haha. Thank you so much

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@조하성-p2d]

​@animatedbiologywitharpan I am really interested in your video! Could you let me know more about the detailed mechanism of the Salting-in step? From your video, I understand that adding salt ions can make the structure of the protein stable along with reducing of the intermolecular replusion of the protein. Then, what is the mechanism of the water molecular can form the hydrate shell around by the protein? Is hydrogen bonding on the hydrophilic regions of the protein? Thank you!!!!

[@aishwaryas4696]

You saved my day within 6 mins🤗 Thank you!

[@animatedbiologywitharpan]

Utilize the rest of the day by doing something creative 🙌

[@wahidahyusof4299]

Best video ever and very easy to understand! i literally understood everything in 6 mins thank you so much! you are an amazing teacher!!

[@animatedbiologywitharpan]

Please share my channel link with your friends

[@pratibhamishra5370]

Thanks you so much dear , I have finally clear my concept with this video . Thanks to you once again .

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@pratibhamishra5370]

@@animatedbiologywitharpan sir please make a video on water and their bond forming mechanism with biomolecules . Please sir this mine humble request to you.

[@krupanidhi912]

Ammonium sulphate precipitation follows the procedure explained by you. It is the good and simple method instead of ultracentrifugation and other methods. Nicely explained. Continue.

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your students . I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@kantamaniveeravenkatanaray232]

Please requesting you send the procedure to me

[@subbud2120]

I can understood so easily, Very good explanation sir

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@a3ministry997]

Thank you ! The concept is clear for me now😊

[@animatedbiologywitharpan]

Please support our channel by using super thanks. Your 2$ donation would give us motivation to do more. Super thanks option is present below any of my videos, a heart sign with $ in it.

[@gisellvm3170]

Very good explanation.Thank you very much!!!!!

[@animatedbiologywitharpan]

In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

[@rebeccaschill4880]

BLESS U BRO ,SERIOUSLY 🥺😭u saved my ass in 12 hours i have my exam and this made me finally understand 💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜💜

[@animatedbiologywitharpan]

In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

[@animatedbiologywitharpan]

Share my channel link with your friends and help me as well

[@shalaka6502]

Thanks alot for the video my concepts are Cleared very well.

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@swatisrivastva4440]

Less time with clear concept great 😊 thanks for this 👍

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@dcroko]

Awesome explanation. You did a finer job at explaining this concept than my professor. Thanks again.

[@animatedbiologywitharpan]

Please share with friends. Please subscribe to my second channel. youtube.com/@letscrackbioexams94?si=ZWdPXv2NSpZeDHZu

[@hmh6040]

Thank you for your such nice and clear cartoon!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@simonclarke2276]

Thank you - very clear and concise explanation!!

[@animatedbiologywitharpan]

Please share my channel link with friends

[@shirharel6520]

1 hour of lecture in 6 minutes... thanks!! :)

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@MehediHasan-zc9dh]

It was very helpful and easy. Thanks

[@animatedbiologywitharpan]

Please share my channel link with your friends and juniors

[@primordial7305]

I love how you teach it. Even a dummy like me can go through medical school just by watching your tutorials.

[@animatedbiologywitharpan]

please share my channel link with your friends and help me to reach big audience

[@crystal_xxi]

FINALLY, I understood this. Thanks so much!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@Hiiraatif]

it was an awesome video. you explained so well. thank you soooooo much

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience.

[@MariaChavez-ey5sr]

Our group just used your video and it was very informative about explaining the topic. Thank you!

[@animatedbiologywitharpan]

Thanks a lot, please support our channel by super thanks. Your 1$ contribution is my motivation.

[@ducktape8520]

Great video! Just have a litte remark: At 1:52 you mark the carboxylate ion which is part of the protein peptide backbone. Instead you should have marked the carboxylate at the amino acid side chain which is exposed to the enviroment.

[@animatedbiologywitharpan]

Thanks for your suggestion 😊

[@abhijeetsurwase9665]

Really useful video. Such a tricky concept made like easiest thank you 👌🏻

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance

[@Gerolinger]

What a great video. Thank you very much

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@roser8325]

Thank you for this video it was explained very well. Got in on the first take!

[@animatedbiologywitharpan]

Glad to know that it was helpful. Please share my channel link with your friends and help me to reach big audience

[@LeonEllisZ]

This makes so much sense! Thank you.

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@susuyao2654]

Thank you for explaining this concept clearly!!!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@mlawliet6151]

Tysm, this was really useful and informative for me. I’ve just had a lecture about protein separation at the university and got confused but your animation helped me so much

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@khadijakhan5290]

Very informative. Quick and easy to understand. Animation make it more clear.

[@animatedbiologywitharpan]

Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

[@joeljamesdn]

Thanks! It was clear and easy to follow.

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@ahmad43754]

You make it easier than I thought, thanks a lot dude.

[@animatedbiologywitharpan]

Please share among your friends and help me to reach big audience

[@ahmad43754]

@@animatedbiologywitharpan one of my friends share it with us in the group 😭❤️

[@animatedbiologywitharpan]

Ahmad Abdullrahman check out all of my playlists....might be useful

[@barathenpillai5497]

what a legend. explained better than my uni

[@animatedbiologywitharpan]

Share in your college group

[@subhabratpanda3673]

Excellent video..m

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@edvaldomaciel510]

Thank you so much for the explanation 🙏. Your wise way to explain alongside the illustration helped me in clarifying some points about the topic.

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me reach out to big audience

[@anzosimon19]

Concept clearly explained❤

[@animatedbiologywitharpan]

Please share my channel link with your friends

[@sarairamirez9272]

best explanation ever!

[@animatedbiologywitharpan]

kzbin.info/door/4IpyopsGWSjaPACNTZLuqg please subscribe to my second channel as well

[@mariamkhan1735]

Thank you! This helped a lot! I really appreciate your videos.

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@onashadissanayake2354]

it was very informative and very clear. Thank you so much

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@uvy3908]

I like your animations it helps give a clearer pictuce

[@bradding578]

great and clear explanation

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@inaayatjahan]

@@animatedbiologywitharpan I just wanted to take a moment to appreciate your incredible work. Your unique teaching style is truly refreshing and inspiring. You have a remarkable ability to present complex topics in such an engaging and accessible way. Your creativity and passion for educating others shine through in every video. Thank you for making learning enjoyable and for sharing your expertise with us.

[@besttricksinfootball9146]

I appreciate your work sir thanks 😊

[@animatedbiologywitharpan]

Please share my channel link with your friends

[@lukachinyemba5619]

Wonderful 💯💯

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance

[@amairaniramirez8855]

Thank you so much! Your explanation was very clear!

[@animatedbiologywitharpan]

Please share among friends and help me to reach big audience

[@NaderSharif-p8p]

Great work buddy keep this up

[@animatedbiologywitharpan]

thanks a lot

[@leonnemulder549]

Great video short and very clear!!!!!

[@animatedbiologywitharpan]

Please share among friends and help me to reach big audience

[@medic_divers11]

full of knowledge

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@walakirajude3529]

The video is useful, thank you

[@linaeline]

Thank you YOU'RE THE BEST👍

[@animatedbiologywitharpan]

Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance

[@TanWeiJunDA]

nice explanation, thank you for explanation its very informative and helpful

[@TanWeiJunDA]

however i have a question, regarding the protein isolation, if i not sure about the solubility strength of my desire protein, then salting in (addding low concentration of salt solution to allow it to solube is better) or salting out is better. And base on explanation, i understand that both salting in and out is helping to isolate, is there any condition like protein that not likely to soluble will be favor for salting out process? if there is, is the example fibrous protein will be more easier to seperate in salting out or salting process? Thank you for answering, look foward to knowing more on this

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@antoniomele436]

This video was very helpful! Thank you! Keep up the excellent work!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@hodahabbal4845]

Thank you .. very very usefull

[@sakshishukla03]

Thank you very much This was very helpful 👍

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance

[@thaynavasconcelos803]

A really nice explanation. A hug from Brasil!

[@animatedbiologywitharpan]

Love from India. Please share my channel link with your friends

[@aq0511]

Very helpful! Thank you!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@AmruMagdy]

Jazak Allah khyra sir

[@claraschmidt360]

Helps so much! Thank you

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@alandbestwn1055]

Thank you,You understood me very well،Keep making more videos🌹🤍

[@animatedbiologywitharpan]

In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Small contribution like $5 means a lot for us.

[@shreyaroy5588]

very nice and point wise explination.Thank you a lot.

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@rajneeshkumar_2067]

Literally bro.. Yor presentation is too good!! Thanku 👌👌👌

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@keniedidi4976]

Great video, thank you so so much !

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me reach out to big audience

[@aneeshrisbud347]

Great Animations

[@animatedbiologywitharpan]

kzbin.info/www/bejne/nXS2fKOEma-ekNE please like this video to support me in a global video making contest (like = nomination )

[@HeLovesMoi]

THANK YOU FOR THIS!

[@animatedbiologywitharpan]

Teresa please share among your friends and help me to reach big audience.....do checkout other playlists

[@ThePerformanceConsultant]

Beautifully explained thank you! I've been struggling with sample preparation for months now - I have VERY expensive triple-labelled samples for NMR so, naturally, sample stability has been a great concern to ensure we can get the most out of each prep. Have you got any rough kind of buffer optimisation "workflow" that you go through when determining protein solubility? I've thus far tried crude hanging drop precipitate screens using a crystallographers microscope, as well as a thermal denaturation assay utilising a hydrophobic residue-binding dye which infers protein unfolding using qPCR (ThermoFluor I believe is the technique name). Have you got any other suggestions? I'm still struggling somewhat with long term stability.

[@Anci3ntDice]

Very useful thank you

[@almaperez8342]

Clear and amazing explanation. Keep up the good work!!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@saharr8723]

so helpful and so concise, thanks so much!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me reach out to big audience

[@saharr8723]

@@animatedbiologywitharpan for sure :)

[@Gabriela79830]

Thank you for this video!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@yasminvie99]

Amazing video!!!

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@bhavishapatel8459]

Excellent video 😃

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@YaqeenAlalawi-v7k]

Nice explanation!👏

[@animatedbiologywitharpan]

Please support my channel via super thanks. Your small contribution like 1$ means a lot for us. The super thanks option is present at the lower right corner of any video, a heart sign with $ in it.

[@fatemehansari1855]

Finally got it thank you so much

[@animatedbiologywitharpan]

Please share among friends and help me to reach big audience

[@malva_fiore]

Explained in easy way, with drawings - the best videos 😍 thank you for your work

[@animatedbiologywitharpan]

In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

[@meghapatil9750]

Well explained sir

[@animatedbiologywitharpan]

Please share my channel link in your college group and help me to reach big audience

[@shubhamsaha6130]

Thanks ! I was looking for salting out mechanism in saponification. I hope the mechanism will be similar, as both protein and soap are polar. Thank you !!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@rekhag-8572]

Amazing videoooo

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@amansajjad11]

Good👏👏

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance

[@ET-ht4zi]

very good ...thank you

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance

[@trisitachowdhury8089]

Great video।।

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audience

[@johanasuh6463]

So helpful thank you!

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance

[@ks99911]

Thankyou sir crystal clear concept 👍🙏

[@animatedbiologywitharpan]

Please share my channel link with your friends and help me to reach big audiance

[@animatedbiologywitharpan]

Also check out my profile in unacademy app there you would get more than 100 free of cost lectures and practice sessions

[@ks99911]

Yes yes I have already viewed many of your free lectures, very helpful thankyou so much.🙏

[@inderdeepkaur6820]

Very well explained 😊Thank you.

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@thilnisha]

Thank you, your explanation was simple and to the point.

[@animatedbiologywitharpan]

Please sharee my channel link with your friends and help me to reach big audience

[@aishj1003]

Finally i understood..thanks 😅

[@aninditakumar3505]

The video is really informative. 👍

[@mustakim2144]

Wow that was a great explanation. it was very easy to understand. THANK YOU!

[@animatedbiologywitharpan]

Please share among friends and help me to reach big audience

[@animatedbiologywitharpan]

Also subscribe to my other channel kzbin.info/door/4IpyopsGWSjaPACNTZLuqg