GST pulldown | GST Pull Down Assay | Protein-Protein Interaction Using Pull Down Assay

[@RafikMachta]

bro 2 min to explain a lesson of 10 pages, you are a goat. Thank you from france.

[@animatedbiologywitharpan]

@@RafikMachta Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@siriansight]

Well done ! This is very, very good. I didn't expect it to be this good. Thx mate

[@animatedbiologywitharpan]

welcome .... I am glad that it helped you..

[@sailikaggallu6970]

Great job explaining it so well and making it so easy to understand! Keep it up :)

[@animatedbiologywitharpan]

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[@hemantgoyal4714]

Really very nice video......... Thanks for your efforts that you made..... Appreciable

[@animatedbiologywitharpan]

Hemant Goyal share among your friends

[@judoqueen5603]

i am Definitely giving a thumbs up. This is simple and clear. Thank you

[@animatedbiologywitharpan]

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[@act_pvt]

I always watch all your adds.. that's my guru dakshina 😊

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[@Free_Falastin2024]

You just explained what my prof couldn't/wouldn't -_- Thank you!

[@animatedbiologywitharpan]

Glad to hear that you liked it

[@animatedbiologywitharpan]

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[@divyasharma5920]

You teach very well, and give unique informations that are important too. Thanks!

[@animatedbiologywitharpan]

Thanks Deepika.....

[@maaz135]

So helpful - God bless you.

[@Menketi]

Thank you, this is so clearly explained I understands how this works now

[@nicolasroussot5742]

Massive thanks for your amazing job ! You are actually better than French biology professor... ;)

[@animatedbiologywitharpan]

In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.

[@chandrimaroy5283]

you have explained it so clearly. Thanks.

[@animatedbiologywitharpan]

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[@arghyajyotipramanik7483]

Excellent. I didn't expect that I'll understand within 2.30 minutes 😅

[@animatedbiologywitharpan]

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[@courtney8690]

Thank you very much. This was concise and extremely understandable. I appreciate it!

[@animatedbiologywitharpan]

Glad that it helped you.....share among friends and help them as well

[@myriamfhal6728]

Hiii everybody ! I made a pull down essay but I dont understand how I am suppose to present the result and how I have to read it. In the video its explain really quickly in the end in 2:10 min. Someone can explain to me ? I made input WB, Pull down WB and Pull down Coomassie

[@yixianwang8025]

This is so good!!!! SAVE MY LIFE

[@francescosilvestro1682]

Speed and perfecly clear!

[@animatedbiologywitharpan]

Francesco Silvestro please share among your friends and help me to reach big audience

[@Paola-cr4qo]

So if a protein A interacts with protein B but we need to know if it is subunit B1 or B2, can we use the same method by doing two pull down assays ( one with protein A and B1 and one with protein A and B2)? Or is there another technique for that?

[@belegend2733]

nobody : searching for gst pulldown on the net ... THE RANDOM INDIAN GUY : VOOOOOIIIIIILLLLAAAAAAA

[@animatedbiologywitharpan]

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[@mumei_57]

Good explanation , i am french and i have understand with any difficult

[@animatedbiologywitharpan]

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[@sammi7387]

You are a hero.

[@pointless_paul]

omfg thank you, hopefull i wil pass my exam

[@animatedbiologywitharpan]

Please share among your friends and help me to reach big audience

[@ooiTzAmii]

Thank you! However, in future videos, maybe it would be better if you could add some possible controls for the technique. e.g. having only beads as a control to identify non-specific binding.

[@animatedbiologywitharpan]

ya i just wanted to keep it as simple as possible ....

[@papridas8979]

Thank you🙏

[@animatedbiologywitharpan]

Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

[@ushaseemaji57]

This helped! A big thanks :)

[@gauravgaur3477]

Great video for research students

[@animatedbiologywitharpan]

Good to know you liked it

[@shivangani9255]

Your videos are really very interesting and beneficial especially your Drosophila related. Are u working on Drosophila? as during my summer training I watched your videos for basics.

[@animatedbiologywitharpan]

shivangani lohani yup I work with drosophila neural circuitry

[@shivangani9255]

Arpan Parichha Ty for all Drosophila related videos as I will be doing my dissertation in this field only.

[@animatedbiologywitharpan]

shivangani lohani oh nice..... you can keep my whatsapp no 9051392160....if you need some help.Where you want to do desertation??

[@naturaldisastertv3298]

thanks man!

[@animatedbiologywitharpan]

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[@amanpadamsey1705]

excellent!! Thanks a million!

[@animatedbiologywitharpan]

If you like the explanation please share among your friends and help me to grow

[@watanake]

Thank you so much!

[@xiBoggy]

great video

[@animatedbiologywitharpan]

xiBoggy thanks ,that keeps me motivated

[@lizflorezp]

omg thanks so much!

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[@AnimeUni-versed]

THANK YOUUUU

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Glad that my video helped you

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[@dhruvsharma2841]

Thanks

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Most welcome! Please share with your friends to help my channel grow...😇

[@ahmadmuhammad.0163]

Make a correction in your video bro: GST is not part of plasmids, plasmids contain genes of interest, not proteins of interest. Genes of interest then encode proteins, here the GST works as bait. GST is added, not encoded by the plasmids as you have shown in your diagram.

[@animatedbiologywitharpan]

GST is a 211 amino acid protein (26 kDa) whose DNA sequence is frequently integrated into expression vectors for production of recombinant proteins. The result of expression from this vector is a GST-tagged fusion protein in which the functional GST protein (26 kDa) is fused to the N-terminus of the recombinant protein. Because GST folds rapidly into a stable and highly soluble protein upon translation, inclusion of the GST tag often promotes greater expression and solubility of recombinant proteins than expression without the tag. In addition, GST-tagged fusion proteins can be purified or detected based on the ability of GST (an enzyme) to bind its substrate, glutathione (GSH).

[@ahmadmuhammad.0163]

@@animatedbiologywitharpan agreed bro, still, you need to use word ''GST,s DNA sequence'' as you you mentioned in your reply. But in video, maybe unconsciously, you used the word GST Protein in plasmid. As you know the sequence will translate into GST protein.

[@shezotetalukder105]

this video is good and helpful but it's to fast, make it a bit slower

[@animatedbiologywitharpan]

shezote talukder these videos are made in terms of global standards..... always you can play the video in 2x speed to slow down

[@AmruMagdy]

Thanks

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please share with friends