Aseptic Techniques: Changing Cell Culture Media

Dr. Thomas Lentz shows us how to change cell culture media in a laminar flow hood.

[@Lwilight]

Get this man a beer, ASAP!

[@anastasiyanestsiarovich3126]

I love your coat!! And the idea of a Falcon tube attached to the triangle bottle! my tip of the aspirate always falls, and there is no place to attach it! I will now also bring the bleach bottle into the hood. 👍👍👍👍 thank you!!

[@anastasiyanestsiarovich3126]

great!!!can you please show how to change the media if the are cell freely floating there? (embryoid bodies for example)?

[@funny11744]

Question : Regarding the stem cell culture in T 75 flask - using media without CO2 : Can we use a closed cap for T75 flask ?

[@blackscreenconversations]

I am getting frustrated with cell culture. I don't know what is causing the contamination

[@julienhe8909]

not professional! all the sample and equipments should be prepared before using BSCs.

[@cowboycatranch]

Media is plural. Most of the time, you are working with medium, not different media. I found the music terribly annoying and distracting (not to mention the laboratory coat).

[@Alliepac]

you sound like you’re fun at parties!

[@theicebooky]

I actually enjoy the music and his shirt made the boring video a lot more fun!

[@poosayfresh8880]

Very much same