This is a tutorial on how to change culture media in a mammalian tissue culture.
Пікірлер: 44
@science_mbg7 жыл бұрын
You should need to edit the title or add in description section about the cell type whether it is attached or suspension cell line. Because if it was suspension cell line, all the cell would be discarded as well by removing the media at first place.
@user-ze9ze3lg1l3 жыл бұрын
i agree with you
@cansutacettin30242 жыл бұрын
totally agreed!
@funny117443 ай бұрын
Do you have any idea how to isolate the URNIE STEM CELLS by simple centrifugation? Usually, the stem cells are mixed with sediments in the morning urine- the best time for obtaining the urine stem cells. I resuspended the sediments -which disappeared at the next centrifugation , but it did not help to find any single urine stem cell. Usually, there are just 5 to 10 urine stem cells in 100 ml of fresh urine. I used a protocol with 500 g for 5 minutes of urine centrifugation.
@el-yugudaabdul-dahiru399 жыл бұрын
The video is educative and interesting. However, if you are not of maintaining the sterility or not too sure of your working environment please revert to using flame at every point of your work.
@JYOtiRaNJanMANgaRaj4 ай бұрын
Thank you so much 😊
@kyoung73111 жыл бұрын
There's no need for PBS if it's you're just doing a regular media change.
@osmirod12 жыл бұрын
The t75 must remain with the cap on at all times except when pipetting
@retna218311 жыл бұрын
KS LEE you mean open the lid slightly of the T-75, after placing in the incubator for air flow? It may be the T-75 where the cap has a filter, so you do not have to loosen it. Cheers
@FireboltRiver11 жыл бұрын
Shouldn't he spray his hands again with alcohol whenever he discards used pipettes under the cabinet?
@jeeva19963 жыл бұрын
No, that quick movement is fine
@12823matthewkao2 жыл бұрын
you didn't wash the culture with PBS before adding the fresh media?
@ibraheemomar81098 жыл бұрын
why are you not wearing a face mask?
@SubhadeepD8 жыл бұрын
great video indeed :) am just start doing my phd work .... and this video will help me :) sterile work = great data/result :) cheers :)
@BigBabySexBobOmb6 жыл бұрын
Oh my God. So many mistakes! Hurts watching that..
@S0m31rishDud36 жыл бұрын
New Years Eve I was just thinking that
@derekdeadlifts29865 жыл бұрын
aren't they students though so they are not professionals?
@jackh95564 жыл бұрын
What mistakes were made? I'm only asking to make sure I do everything correctly.
@ppottokki3 жыл бұрын
Pouring media onto cells...and no flame sterilization.. Flame sterilization is necessary while you put foreign materials into the clean bench.
@funny117442 ай бұрын
@@ppottokki Not any materials could be flamed ! There is also cold sterilization.
@toshibacoast12 жыл бұрын
good video.
@abumosabelhindi550512 жыл бұрын
its very nice
@ubanakoblaise913811 жыл бұрын
No need to wash cells with PBS?
@funny117443 жыл бұрын
Please mention what type of cells are ? Are human cell ?
@amrutasudake72716 жыл бұрын
In how many days we should change media
@funny117443 жыл бұрын
Mention 2-3 days.
@moonlikeyou3339 Жыл бұрын
How long do you change the medium?
@vadersfist1211 жыл бұрын
what kind of bracelet was he wearing? is it sterile? I think not... for shame, sir. for shame.. :( not :D
@kslee809612 жыл бұрын
Why not open partially the T-75 in the CO2 incubator in the last part of the video?
@kannanrk1007 жыл бұрын
KS Lee it's a vented flask
@Carl-Ernst-Otto-Kunze Жыл бұрын
@Zauberberg
@tulsijoshi1046 Жыл бұрын
If she discarded the media won't the cells go with the media?
@eversincekorea Жыл бұрын
the cells are adhered to the flask
@funny117442 ай бұрын
Only the dead cells go with media, the adherent cells are fine. Which is good for the healthy of last one. I think.
@HuongPham-ur1sz11 жыл бұрын
làm sao để xem dc đây
@lemyafinder2898 жыл бұрын
i'm looking for a cell culture lab for an internship..can any one help?
@vadersfist1211 жыл бұрын
My bad I meant to dislike this. CSU Pomona does it hood status!
@Gangularis8 жыл бұрын
Why are you wasting your time spraying the pipette packaging? You aren't going to get it close to being completely sterile, and that wrapper should never be over any of your open containers, anyway.
@funny117442 ай бұрын
just for precaution.
@infinitefuture18283 жыл бұрын
You shouldn't wear socks while performing experiments
@funny117442 ай бұрын
it depends on how skilled you are and it depends on the cells.