Analyzing DNA Sequences Using MEGA & BLAST

Recorded with screencast-o-ma...

[@suvigyasharma5474]

Hi... Lisa ..when I opened this video I was hoping to get instructions to build a phyoT using different fungal DNA sequences with Mega...but this tutorial was also helpful...nicely explained the way DNA sequencer works

[@isharajwade4610]

Fantastic video. Very well explained.. Thanks Lisa! 👏🤩

[@simonpender8331]

Love it - "drink milk, read books" - great advice.

[@kamdynjaxon8479]

you prolly dont care but if you guys are bored like me during the covid times you can watch pretty much all the new movies on Instaflixxer. Been streaming with my girlfriend these days =)

[@jerrymekhi6647]

@Kamdyn Jaxon definitely, I have been using instaflixxer for since december myself :D

[@nguyenhuynhnga4635]

Thank you so much.

[@user-vh4pt1ws2w]

Very useful. Clear instruction!

[@bidwansekhar]

❤

[@monalizapumihic9538]

Thank you so much💗

[@nohataha6398]

what if when using blast for unknown it gives 75 similarity identity?

[@collaniedog6692]

this is what I want. Thank you for this video.

[@Azrael928]

Nice video, but could you teach how I can change or delete letter in an abi or scf file?

[@fasdz5533]

If i had a point mutation in my DNA sequence, can i determine wether it is nonesense or frameshift by analysing sequence data?

[@armwanniwat9369]

thank you so much

[@hba3820]

This is so helpful, thanks a lot!!

[@yokaisamaful]

What if i get several alignements with 100% Query cover and 100% Per Ident after blasting my sequence in NCBI? what does it mean?

[@Bigtigerfanmom]

Could you rephrase your question, please? I am unsure of what you're asking.

[@ashabhardwaj1920]

Hi. What should be the percentage of identity so that we could 100% sure about particular species and how do we know about new species?

[@Bigtigerfanmom]

I checked with Dr. Chris Hittinger of UW Madison's Genetics - Hittinger Lab. This is his response: I agree you can’t be 100% sure without a 100% match. If it’s not a 100% match, it could be a sequencing error (which could be checked by looking at read quality or redoing it), or it could be natural variation within the species (very common). For it to be considered a new species, several criteria would need to be met. If it were substantially different (

[@nemo9396]

How do you identify a gene out of a sequence?

[@Bigtigerfanmom]

Look for patterns. Something looks amiss visually.

[@aqili.n5075]

please where can i find this data (yHKS1.ab1) thanks

[@amalienaahmad6353]

hi. how to read the blast result? I mean, how i know if we discovered a new species from blast? based on your video, if the species already found, then the result shown the report mentioned that it have been sent by someone...but how the blast result show and report if it is new discovered? i just dont understand how to read it. do you have any tutorial on using blast? thank you.

[@Bigtigerfanmom]

Hi! Good question! Once you've 'blasted' the results, if there is no close "hit", YOU may be on to a new discovery. How cool would that be? Name the organism after me - ha ha!

[@prestonmartin8268]

Interesting. I came a little bit

[@Abyie-Ho-dam]

evolution? You people who study this think all this is random? mwahahahahahahahah