Caffeine extraction from coffee ☕🗜️☕

Welcome to what is undoubtedly by far the most laborious project I have ever done, which I think is the most large extraction of caffeine using this method on YT. It has been four weeks of extractions and editing advancing step by step each day. I hope you appreciate my work as well as enjoy it ;)
Let me know what do you think about my yields compared with other people!!!
Flute players, sorry for using that cleaner as a stirrer, dont take it into account. Anybody saw me at the end of the video? :D
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Please rate and share if you like. Subscribe for more video uploadings.
¡¡¡THANKS FOR WATCHING!!!

[@stopthephilosophicalzombie9017]

Separatory funnels are so beautiful.

[@Young_Ruya]

your videos remind me of my college years. twenty years ago... i brewed rice wine at home recently so i searched proper distillation process with my home appliance but i miss so much clear glassware you use in this video. thank you.

[@Glattuh]

You can always purchase a distillation set up! Id suggest to watch my latest video if you like distillations too

[@Young_Ruya]

Nation Of Our Hobbies you're right. but probably that's so much work to do with those glasswares used in labs ^^ ... the amount of my rice wine is almost 10 liters! so i decided to make simple distillation tool by myself with some copper pipe and a big plastic basket and silicon hoses... but i love your distillatin videos so much i already watched and subscribe your channel. from Korea

[@Glattuh]

Nice project! And thanks for subscribing to the channel, good to see people come from everywhere around the globe!

[@Conex145]

You can use Ethanol to extract the coffein --> filter the slurry --> add some MgO to adsorb some of the fats/impurities --> filter and then boil of the EtOH --> dissolve the residue in hot h2o --> filter again --> add some acid --> extract 3x with solvent of your choice (DCM preferred) to remove so alkaloids (they stay in the water layer) --> remove solvent --> crystallize from hot water ! So you'll get the cleanest product without column chromatography or sublimation

[@blaquerDP]

Caffeine* and no, using ethanol would be dumb as heck. You just add extra steps (and solubility in EtOH is much lower which makes the method absolute trash ) Also who would even think of column chromatography or even mention it when the product can be recrystallized...

[@donbolillo3812]

nope, you should try your own method and let us know how it works

[@Prchemist06]

You videos are relaxing plus informative .

[@eriktorres2000]

I really like your videos! especially the ones about chemistry!

[@Glattuh]

Thanks!! Much appreciated!!

[@sietseaveresch8480]

This background noise is weirdly calming. Excellent video 👍

[@Glattuh]

Thank you! It is the housebrand hehe

[@navalkishore502]

I am enjoying your videos and it is actually enhancing my knowledge .. about the component we use day to day

[@Glattuh]

Nice to hear that!! If you liked it, check out my other chem videos too!

[@goknil4099]

Thanks a lot. Great, amazing video. I am having hard time purifying the crude caffeine so this video is rlly useful. Thx again

[@jeremyg1833]

02:50 never realized how similar caffeine structure was to a purine. adding the structure was a nice touch. thanks

[@Glattuh]

In fact is a purine itself! A trymethylated one by the way. So does theobromine which can be found on chocolate and has similar effects :D

[@amiralozse1781]

very well done!!

[@MrCodix]

the sound of the ocean is so soothing

[@khavooffical4961]

So Amazing skill. thank you so much. I hope you will make more research about coffee.

[@Glattuh]

Thank!! But i dont know if I can do anything more with coffee ;(

[@user-zp2mh6bj8i]

Wooooow. This is really long way. Respect

[@adhikery2]

cool...... learning a lot

[@Idk-bs6in]

This takes me back to the lab days Remembering the stuff we learned, without having a real understanding at first But eventually it became routine, and now I look back on those days, things were so simple But that second semester of organic with death stroke professor, wow did you have to include everything, the reports were brutal, not to mention the questions were taken from old topic specific books so no one could easily find them A ruthless semester… but we all rose to it… mostly… Kinda like the ant hill thing in pysch, the style Well only Looking back because I’ve found the fault in the chain I was looking for… in my daily coffee prep Still… wanted to see what else I could possibly be missing, if not, what else I could improve to exceed the standard I had just achieved And found an interesting channel on extracting caffeine from coffee, and it based on hobbies… this is brilliant just what I’ve been looking for, I’ll be sure to check out more videos, as hobbies are something that seems to have fallen out of favor in the last few years Also I’m still up in the air about caffeinated coffee and it’s negative effects on nitric oxide production on the body Slow progress on the 1,000+ compounds in a cup of coffee

[@anonymousandy2789]

wouldn't this extraction yield a full alkaloid profile? i know that the flavons and antioxidants would be stripped, but the trace amounts of non-caffiene xanthine alkaloids should be in that final product. i would like to see this done with Mitragynina Speciosis leaves, very closely related to tea but contains 7-OHM, a tryptamine like molecule that binds to mu-opioid receptors and produces hydrocodon like effects without danger or histamine release of traditional pain medicine

[@alish5417]

Great video well explained thank you

[@Glattuh]

Thank you for watching!!!

[@AweSomo84]

very interesting and very amazing skill set

[@Glattuh]

Thanks you¡¡¡¡¡ Check out my other videos, maybe you like them too :D

[@ashW110]

Well presented process and the video design creates a really pleasant atmosphere. In one of my videos, where I extracted caffeine from coffee, I followed a similar process. However, I didn't use an additional source of acidity as coffee naturally contains many organic acids. The pH of the thick coffee mixture with water can be as low as 4. I believe this should be sufficient to protonate most of the caffeine and make it very water soluble. However, my extraction was conducted under the different circumstances and different coffee materials (instant coffee). Do you think you could get away, without HCL?

[@Glattuh]

Thank you¡¡ Apreciate the comment :D Yes of course, it can be done without adding HCl. I just wanted to follow a different way than the rest of the people. I didnt know how acid can coffee be itself. I wanted to add HCl because in other extraction I did previously, some caffeine were decomposed during the heating process (during the recrystallization I found that caffeine were dissolved in hot water but then never crystallized. In fact i let the water evaporate and I found some caffeine agglomerates in a viscous oil medium; and that was from relatively pure caffeine crystalls). By adding HCl I was able to increase caffeine solubility in order to not apply so much heat. Anyway, like it is written in the video description, I always want to know how my yield is compared with you, the internet people. Thanks for commenting :D

[@ashW110]

Oh, thanks for the explanation. It totally makes sense! Also, I believe because you are extracting from the nonsoluble material, HCL gives this extra bit of power to protonate and dissolve caffeine faster. In any case, well done! Will be looking forward to your videos.

[@Glattuh]

I will never say it better. The HCl is exactly doing what you said, giving that extra bit of power :D Thanks for your support!!!

[@MoyshaYakovlich]

I haven't captured your name as I don't think it is anywhere in the video, but thank you for sharing your passion! I am a chemist by education, but I never really thought of running any of it at home for some reasons (save for those youth cases when I'd electrolize NaCl aq. in my room overnight just to wake up with a terrible hangover) until very recently. I like your creativity in some situations, I see you have that home-made retort stand with a concrete brick for a basement - what an exellent idea! You can never be too save with those stands... :) And using the rubber suction bulb to create vacuum for filtration is genius, I might use it in my lab one day too! Now, I have a question to the subject. As you've correctly mentioned, caffeine is not the only compound extracted by HCl aqueous solution. In fact, you are brewing a coffee with all its flavoursome (and not so) components, which are a lot. So, how can you claim anything about the final yield? Out of those 0.27% the caffeine's fraction can make anywhere from 0 to 1. Cheerios!

[@Glattuh]

Thanks! Yes absolutely, I would have loved to have acces to an HPLC instrument to see how dirt and rich in other compounds my caffeine was. I asume it is mostly caffeine as in the last step a recrystallization is performed, but yeah, as you said, many other compounds can be present there. I have a video planed to purify it by sublimation, will upload it some day :D Thanks for commenting fellow!

[@sciencek]

I love your vacuum system

[@Glattuh]

;)

[@sciencek]

Nation Of Our Hobbies Where do you get the vacuum filter set ?

[@Glattuh]

Well, I get it by pieces in labbox.com It is not sold as a kit or something, I just connected few diferent pieces

[@sciencek]

Nation Of Our Hobbies OK Thank you

[@donalkevin6901]

Hi Nation Of Our Hobbies. This such a great video. I like the detail and the quality of the video also. I hope your channels gonna be more famous. And just want to ask you is this same procedure if we want to ekstrak the alkoloid from Ma Huang ? Hope you reply. Thanks!

[@Glattuh]

Thanks for your comment! I am surely growing in numbers yeah! Many people ask me that lately. I didnt make any research about that but this is a more specific method, as I use some HCl and so... But can guess that it will work for other plan materials too!

[@RaExpIn]

Wow! That's a lot of effort to get 1 g of caffeine. Nice work!

[@Glattuh]

+Random Experiments International Thanks!!!

[@adammorgan1776]

Good video and the extraction. Do you have a list of chemicals and equipment used for the extraction?

[@terriatca1]

This is why I buy my extracts from companies that make them. I make soap and if I tried making these extracts on my own I couldn't sell them as the price would be astronomical.

[@tamaz88]

HCl is the yummiest part 😋

[@timothydestiny3865]

Subscribing right away! Thanks so much

[@g-radical349]

Awesome video, did you ever wind up posting the recrystallisation? I'd love to see the structure that slowly grown caffeine crystals take on

[@Glattuh]

Hi thanks! I have a video planned for purifying it but with a much convenient method: sublimation. I may do a recrist after that to show you the crystalls yes, but the clip you see at 13:38 is pretty much it

[@varunravi6156]

hi, any chance you can share the link for the solubility data?

[@Suzuchikuma]

I didnt expect that you poured all packed of table salt to separate emulsion

[@dwikafebrianto3016]

what coffee did u use? Arabica? Why did the caffeine content suck? ~250mg/500g. I thought I would get approx. 100mg of caffeine if I drink 20g of coffee. That's why caffeine supplements are more potent (and also potentially more dangerous) because they use anhydrous form of caffeine in their products

[@0xstev3]

Does anyone know where I can get a pipette pear in the UK? I've never seen those before and I'm having trouble souring one.

[@Glattuh]

Try to search it as pipette filler ;D

[@Holistic-Healthcare]

Ebay :)

[@5commandomerc]

Brew coffee, open several cans of diethyl ether (aerosol starter fluid) toss the whole mess into 2l pop bottle, shake that bottle, let settle and separate, isolate ether, evaporate either(you will probably get high)...if you haven't exploded, congratulations on your caffeine isolate.

[@gobattery1627]

What is the Glass Piece used with the Pipette Pear to make the Vacuum?

[@Glattuh]

It is a suctio adapter for a jointed buchner funnel. In this case with 14/23 joints both female and male

[@sciencek]

How do you make the molecule's animation in your video? And with what software ?

[@Glattuh]

Hi! I use Avogadro to make the molecules and its rotation and then use Fraps to capture the video. I just use some bright green or magenta background to then apply the chroma in the video editor ;)

[@sciencek]

Nation Of Our Hobbies OK thank you because i want to make a video with a molecule's animation.😊😊

[@DamianAI9]

Really nice video i have try it with Caffeine Pills . Try to make a Withe or Black Background Buon Lavoro ;)

[@Glattuh]

Thank you¡¡ Im moving to another place to record all this lab videos, will think about that ;D

[@DamianAI9]

Nation Of Our Hobbies Nice ;) Take an example of NileRed on KZbin he has made videos on caffeine to

[@stanleykafara3826]

Hay what do I need to do a extract with a green plant...I ur there for caffeine extract the same is green plant...its called wild lettuce

[@imfllirymydude341]

Hello, I am unsure if you still respond to questions on this video but I thought I'd give it a shot. I'm going to do this experiment as part of my end year high school project but my teachers preferred I did not use DCM. Would ethyl acetate work as a substitute?

[@Glattuh]

Hii!! Suree, it is better in fact, less dangerous. In fact it is easier to crystallize from ethyl acetate by cooling a solution of it ;D Good luck in your extraction!!!

[@imfllirymydude341]

@@Glattuh Thank you for the very quick answer :)

[@whtRk]

@@Glattuh how about using acetone?

[@emlakes]

Why don't use more DCM for extraction?

[@saniamahveen]

When there is such lill caffeine in coffee...like only 271mg in 100gm of coffee...Then why is coffeee blamed for its caffeine components and regarding health issues...bdw..i love watchin ur videos..very interesting to watch.. hope u reply

[@Glattuh]

Well the quick answer is that regarding of its low content it is powerful enough to make you excited, awake and even nervous, and that can lead to health issues of course. Just to mention, these are my own results and are based on dry coffe grain, not the liquid you drink. And thanks for that!!

[@jorgeandresviccomateo5914]

Could I use NaOH instead of sodium carbonate as long as I use the correct amount?

[@Glattuh]

Well in theory you can but hydroxide by itself is a much more strong base and also nucleophile so it may degrade your organic compounds, not even caffeine but it can make the whole process much more difficult ;( From my point of view, carbonate is cheaper and more safe to use so its a goto in this experiment (and in many others for the same reasons)

[@TheSoundshift]

How to extract nicotine from tobacco?

[@thecreativewebshow]

i dont think the nitrogen at 3:00 is protonated. the imine nitrogen is more basic (resonance stabilized conjugate acid) so this is the one that should be protonated.

[@yukuzakymomonosuke]

why that nitrogen i more acidic than the other one in the imidazol ring? the lone pair is supposed to be involved in the aromatic delocalization while the other one has a lone pair in sp2 orbital i don't really understand, if someone knows please I really wanna understand this point

[@mreese8764]

And this is why people have their caffeine be prepared by professional baristas. It's a lot of work.

[@Glattuh]

Well, I am a bit confused. People there prepare coffee not caffeine. Caffeine is the main alkaloid responsible of making you active and excited. Caffeine is extracted industrially by superfluid extraction. This is done by chemists :D

[@tyt0uoff146]

how did you acheived to obtain labbox glassware ? It is restricted to professional use only

[@Glattuh]

I run a chem YT channel. But in reality i dont need it anymore, have a good sponsor that provides me with everything I need :D

[@philoupaulo]

I'm very interesting in chemistry but is it the best way to make a good café ?

[@Glattuh]

Hi. This method is not to make coffee but to extract one if its compounds and purifying it. The liquid is no longer edible once it touches chemichals such as DCM which is an organic solvent. You can think I am """decaffeinating""" the coffee but, as I said, it is not longer edible, both coffee and caffeine extracted.

[@MoyshaYakovlich]

@@Glattuh why not? Even if a simple under-the-air drying does not remove all of the solvent from the crystal matrix of caffeine, it can be either dried even more deeply under a good vacuum to reduce the residual solvent content to a safe level, or washed by a food-safe solvent such as ethanol.

[@cloudslady3400]

What is DCM?

[@kostyk-kostyk291]

I would like to see the synthesis of vanillin or ethyl vanillin.

[@farm.miriamavila5003]

no tienen es españa restricciones con el ClH, carbonato sodio acetona, metanol?

[@Glattuh]

Hola¡ No existen no. HCl, acetona y metanol se venden en muchos supermercados como productos de limpieza, normalmente en forma diluida El metanol por ejemplo forma parte de lo que se conoce como alcoholes de quemar (de coloracion azul). El carbonato de sodio no entraña ningun riesgo y tambien se vende para limpieza, con algunas otras aplicaciones. Un saludo ;D

[@farm.miriamavila5003]

@@Glattuh en argentina, aun siendo farmaceutica, hay restricciones de compra, una verguenza. gracias x tu respuesta

[@jhyland87]

Must be awesome to be doing chemistry right on the beach.... lol

[@Glattuh]

I wish I could haha. It is my style I guess! About the other comment, well, this videos have time actually, but thanks for commenting. Check the new ones if you are interested! Glad to see you discovered my channel, thanks for stopping by! Bests, NOOH

[@jhyland87]

@@Glattuh For sure. This is a great video! Much more detailed than most other caffeine extraction videos. Maybe try doing a similar one for extracting piperine from black pepper? Or extracting capsaicin from ghost peppers? Just a few ideas. Regardless, keep it up! I plan on starting a channel similar to yours sometime in the near future, hopefully. I have a lot of cool chemistry experiments that I dont see covered very well in YT videos. Thanks again bud.

[@Glattuh]

Yess I will do them for sure!

[@jhyland87]

@@Glattuh Do you know anything about _pseudohalogens?_ I think that could make a cool video... Showing how a compound containing a halogen could have the halogen substituted with its pseudohalogen but still have nearly identical traits/attributes. Learned about them when researching Fulminates

[@Glattuh]

Wow never heard about them! I will look for it for sure, that is not a common thing I guess

[@i_dont_get_out_much2859]

Is it food grade?

[@Glattuh]

Hi¡ No it isnt. Nothing is food grade in my opinion if you made it by yourself instead of a certified laboratory or company. I used organic solvents and also an acid to recover it. The video is just to show you how I extracted the caffeine ;D

[@kaspervankan583]

why was it necessary to make the caffeine basic for the rest of the extraction?

[@Glattuh]

Hi! Caffeine needs to be in basic aqueous conditions in order to be extracted because it is an alkaloid, which among other things means that it is alkaline or basic. If an acid is present it forms a salt which goes to the water phase instead of the organic one. By addind a base, in this case a carbonate, we force it to be in its free base form, which can go to the organic phase in a higher propotion.

[@zeeshantn5934]

I was done this expriment

[@gayusjayusable]

next, please show us how to extract flavor concentrate from fruit, not the oil but water based solution... please..

[@ihmwmleanfanger2352]

Why he/her dont say Level of Hydrochloric acid. and tee.

[@alaskanalain]

Doesn't work.......I keep drinking it before completion

[@DruggiePlays]

Oh boy, drinking coffee with HCL? Good luck

[@macjimenez1255]

Do you need chloroform for the determination of the concentration of caffeine?

[@Glattuh]

Chloroform is the same as DCM, but whit one chlorine atom instead of an H. DCM and chloroform are pretty similar and both will dissolve caffeine, so yes it can be used.

[@getmeoutofsanfrancisco9917]

Why was your yield so low? You got a 20% yield out of a theoretical 5g (+/-) yield. This is of course *assuming the coffee was decent strength.* Using "decent strength" (USA) coffee (subjective I know) as an example, 1 gram of coffee usually has at least 8-10mg of caffeine (or 1000mg per 100g of coffee) in it, giving it 1% caffeine by weight. It is not uncommon for individual cups of coffee to have 100+ mg of caffeine (at least in the US). Was your coffee source weak? What roast did you use (Dark/Medium/Light)? Do Americans just drink insanely strong coffee? What gives? This isn't the first low yield I have seen, it seems to be a pattern regarding caffeine extraction. Anyways, I love the vibe of your videos.

[@Glattuh]

Thanks for commenting! I am glad you found a pattern across this method on the internet. What is clear is that the method of extraction is not so effective, plus, it could be a mix of the other variables you mentioned. On the other hand keep in mind that this is not an analytical type of chemistry, just an amateur extraction. If you really want to know how much caffeine is within certain coffe, you would need another method, techniques and work with very small amounts, not a whole kg as I did.

[@michaelfettes5479]

Pablo Escobar!

[@whtRk]

is it okay using acetone instead of DCM?

[@Glattuh]

You can not! For liquid-liquid extraction you need two liquids that are not miscible with each other. Acetone is miscible (soluble in all concentrations) with water so you wont observe a separated phase

[@whtRk]

@@Glattuh how about using ether?

[@Glattuh]

That's another story! Yes ether can be used too

[@whtRk]

@@Glattuh thanks for answering late quation

[@Dariusuzu]

make a video how to extract ephedrine xD

[@flyawayat8746]

🌹❤️👍🏻

[@anonymousandy2789]

the species of coffee and the roast maters with caffeine extraction. Im assuming that you used roasted Coffea Arabica, optimally you want to use unroasted (or blonde roaster) Coffea Canephora (robusta) beans. robusta is higher in caffeine and lower in libids. but it has a lower acidity. try to find it ethically though as monocropping and deforestation are frequently used to produce robusta. the coffee you are using is bred to taste not extract, but Cofea Canephora tastes terrible due to its high caffiene content. its mostly used in cheap instant coffee packets

[@NoahKainWhittington]

I bet you could extract even more if you had a Soxhlet extractor.

[@Xc8532]

How would this process look? I am looking to do a school project

[@-COBRA]

Try to use magic button TARE on your scale.

[@wernerhiemer406]

A scale has a maximum capacity. So one can only weigh this minus, well the tare. Brutto (capacity) minus tare (the container even a piece of paper for powders etc.) makes netto (what you actually weigh). In other terms a scale is not a calculator even with the count button. Maximum capacity still applies.

[@kylepoliah6272]

What is DCM and what was its function in the extraction?

[@kylepoliah6272]

@yabon banania I eventually googled it. According to science direct its dichloromethane and its a common solvent for extraction.

[@mostafaadnan6553]

Try sublimation to get more pure caffeine

[@Glattuh]

Thanks for the idea! Will be an interesting video to film :D

[@jhyland87]

Great video/content! One problem though.... A _twenty two second intro_ of just your logo.... seriously?...

[@kongkiatc]

caffeine is alkaloid. so nicotine is alkaloid too. Can i use this process for extract nicotine? Want you to show extract nicotine Please

[@AikkuKun]

Nicotine is a volatile liquid at room temperature, and gram-for-gram a ton more toxic, so not the best idea. It also readily oxidizes on contact with air into cotinine. Solvent extraction of nicotine generally works by placing finely-shredded tobacco leaf in a base solution (as the nicotine present in plant material is generally a salted form, so this liberates the freebase), then performing a steam distillation using super-heated steam injection (around 250C), and the resulting nicotine freebase dissolved in a suitable carrier (eg. kerosene). Then it's cleaned and re-acidified for transport (as the salts are generally less volatile). Again: Nicotine is /insanely/ toxic relative to caffeine on a weight basis, and it's a volatile liquid. If you have to ask how it's done, you shouldn't even consider attempting it.

[@kongkiatc]

@@AikkuKun Thank a lot. but I need to extract nicotine for e-cig ( I want to quit smoking). Can I use ethyl alcohol instead of kerosene?

[@AikkuKun]

@@kongkiatc If you need nicotine for e-liquids, you buy it pre-made (generally 12-24mg/mL when ready-to-use, 80-150mg/mL for diluting with PG/VG/PEG/DH2O, etc.). Concentrated (and especially: pure) nicotine should only be handled by skilled chemists in professional laboratories with all safeties in place. I seriously cannot stress this enough: *Nicotine in its pure and/or concentrated forms (and especially as salt) is absolutely /deadly/; do NOT play around with it.* It should never even be present outside of sealed environments in a professional laboratory or processing facility. Even tiny amounts (2-3mg) are enough to make you sick, and because of how volatile it is (especially during the steam distillation), you would be exposed to a lot more than this without professional equipment and training, and you might not even realize it until too late, at which point you may collapse and need urgent medical help to avoid organ failure. I only answered as a curiosity in regards to the chemistry in play; it was - and is - NEVER meant to be performed, and is only for educational purposes. All that out of the way, and again *only* for educational purposes: Freebase nicotine appears to be somewhat soluble in both polar and non-polar solvents but moreso in polar ones (ie. water), which may explain the need to use superheated steam distillation; EtOH would 'work' about as well as any other solvent, but I imagine that it would also evaporate the nicotine if you attempt to dry it (it might be possible to dry a solid nicotine salt under vacuum, but most of them are liquids afaik, and I'm not sure how stable they are; they might decompose into freebase + acid mists).

[@kongkiatc]

@@AikkuKun Thank you very much for the advice. And that worried me I will follow your advice.

[@beyzanurylmaz1212]

imagine people starts sniffing caffeine instead of cocaine. but just imagine dont do drugs guys .

[@danarchy723]

Have you ever tried chloroform (trichloromethane), instead of dichloromethane. Caffeine is supposed to be way more soluble in chloroform

[@maryurbina7091]

wtf they are recording in beach o that shit

[@lens3973]

Yeah you shouldn't have put the lid on when adding the carbonate. A gas producing reaction in a closed vessel = a bomb.

[@00001111qqqqpppp]

Brine solution works better for organics less dense than water. Adding salt causes the density of water and DCM to become to similar and makes separation even worse. DCM emulsion are best solved with time. If you want it to be fast it is better to add more solvent. Check out this website its amazing for chemistry www2.chem.rochester.edu/~nvd/?page=home

[@edgardpalacino9783]

Of course. This is a very hard process. The amount of caffeine is ver low.

[@Glattuh]

In fact, I extracted a lot of caffeine using this method. I did not found any video at that moment that extracted 1000mg of the desired compound :D

[@SciDOCMBC]

You are making a false assumption here. DCM forms emulsions with alkaline solutions much more easily. Unfortunately, by adding more sodium carbonate, you did exactly the opposite of what you intended.

[@pintukhan3556]

What da hell mane you dont even explained

[@mackychloe]

read much?