Column Chromatography Basics and Lab Procedure

In this video I have discussed about what is Column Chromatography, what is its principle and lab procedure and How to perform Column Chromatography in lab manually step by step.
First step is to choose the stationary phase based on sample.
Second step is to pack the column, packing can be done by two method 1) Dry method 2) Wet method.
Third step is to choose Mobile Phase
Forth step is to load sample onto the packed column. Sample gets purified on the basis of polarity.
Later we have also look in brief at isocratic elution and gradient elution.
Please watch this video for more detail. If you like this video please like and share this video, and don’t forget to subscribe and click on bell icon for new videos.
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