Copy Number Variation - How Does It Work? - Ask TaqMan #34

[@ismhj4707]

There is a sign mistake in one of the Delta Cts at 2:06, please correct it. Thank you for the video.

[@CatalinRusnac]

Yup, dCT in the calibrator should be -0.5, but they probably mixed up the Ct(FAM) and Ct(VIC) values

[@nathaliemorales1227]

I want to know where you got that golden pippette! I want one!

[@ViktoriyaMandrusova]

Hello, can you explain how you got ΔCt 1.5 and ΔCt 0.5? in the comments they say that you have a mistake (not 0.5, -0.5) and therefore the final result is not 1, but 0.5

[@4Getf00l]

How is copy number of a gene analysed? I mean how can I know the copy number of a specific gene?

[@thermofisher]

Thanks for your question Maimuna. When calculating copy number using our assays, there is the use of a reference gene where there is a known copy number. For example, in humans, RNase P is known to have a copy number of 2 under normal situations. You are using that known number to calculate your unknown target's copy number. For additional technical support, please reach out to us at thermofisher.com/askaquestion.

[@MonoTheLuke]

Thank you

[@bientran6391]

how did you do it can you share with me , thank you

[@ellawynn1644]

How would I go about finding specific information due to a 1.6 Mb deletion at 12p.13.32 (3615205-5225595) with at least 18 genes being affected by CNV?

[@thermofisher]

Hi Ella, Thank you for your question. Please contact our technical support team at thermofisher.com/askaquestion for additional information. Thank you!

[@Chicagomom]

How to find a calibrator sample for my gene of interest?

[@thermofisher]

+fan wang Thanks for asking! Check out this post in the AB Community for information on calibrator samples: abcommunity.thermofisher.com/community/real-time_pcr/blog/2013/08/16/how-to-find-a-calibrator-sample-for-a-copy-number-experiment

[@anomalyp8584]

Linus from the genetics department

[@shixiangwang]

It is too fast.

[@shixiangwang]

But it indeed a good video. Thanks.