Cre-LoxP Recombination

[@thathaw7769]

ONE DAY BEFORE BIOTECH EXAM, ANYONE? Thanks so much for the clear explanation, you save my grade!!!

[@efejordan19]

Same here

[@miszajansen]

Yup lmao

[@moistschmeckles400]

2 days before molecular biology, hoping for a miracle

[@kkmarskk]

One of the best among the uploaded videos

[@zunwang2214]

Have been using Cre mice for years, never know how they really work. Thanks a lot.

[@bal0423]

oh this comment gives me a relief lol

[@gadiscymraes]

Really clear explanation! Thank you so much!

[@Emmanuelbrick]

Hi, thanks for the video. It would help if you place the references in the video description, so we can research it easier.

[@muhammadaliarif-artist6543]

Excellent presentation

[@betneha]

thank you for the good explanation. 🙂

[@-mera-9161]

Concise but informative. Thank you so much for this useful video!!!

[@katharina839]

Thank you very much for this perfect explanation! Can you tell me where you got the information that 'lox' means 'locus of crossover' and 'Cre' means 'creates recombination'? I would like to cite it, but I can't find it anywhere else...

[@ahmadneuro_world6173]

Many many thanks to you for creating such a useful video!

[@jannelis2845]

To how large of an area could you introduce the Cre-LoxP System in vivo given the tissue is easily accessible such as epidermal tissue?

[@peterfruchtig5334]

Very clear video! Thank you very much!

[@MonkeyDLuffy-xr4fl]

Thank you, brilliant explanation!

[@faisala.younus6223]

hi give me the PDB code you used to map out the residues of Cre-recombinase

[@dmoakes]

Exactly what I needed.

[@ameliac504]

Excellent work

[@PJ-lb5jr]

this was great. very well written.

[@pennyroyal7383]

Great explanation

[@alexandrekisner5369]

thanks Hannah, this video is great

[@زيادبنالمحمد]

How to interpret the results?

[@sarahpark6245]

life-changing

[@teyanera594]

merci beaucoup pour cette vidéo !

[@french4896]

- Recombinación Cre-Lox es un sitio específico de la tecnología recombinasa, usada para hacer deleciones, inserciones, translocaciones e ---inversiones en un sitio específico de ADN en las células - Cre- Para que se transloque (enzima) - Lox- Para que el sitio específico se exprese correctamente - Recombinasa- para permitir esa modificación genética - 2:07 genes en el mismo sentido (1); cuando están en sentidos opuestos están en bucle e inversan el sentido del gen, el problema es que se giran pq es reversible, por eso se desarrolló el PLox (2); en la translocación, la secuencia no está en la misma hebra, por eso no está en el mismo sitio de translocación - Con CRISPR CAS y CreLox se usan para hacer knock out/in + Optogenetica - estimulas cuando quieras + Optogenetica - cuando tienes el farmaco se va a estimular continuamente pero no tendras el control (ratones trans + adenovirus - expresa Cre en cierta poblacion, nomas donde esta Lox y ahí se expresan las rodopsinas) --> Diferencia es la resolución temporal FUNCIONES - Linaje de células (como en las celulas pancreaticas)

[@RadhaK2697]

Can i have the accession number from ncbi or pdb to view its structure in more detail..? Thanks in advance.. :)

[@Mazharvlogg]

beautifully explain .. many thanks

[@Alex-hh9tw]

Great video!

[@darirfan8122]

informative and precise. thanks!

[@alphamonkey1234]

how to introduce the LoxP sites so they flank the gene of interest?

[@nanas273]

I guess you can use CRISPER

[@alphamonkey1234]

​@@nanas273 lol

[@piccolo917]

fking life saver, THANK YOU!

[@Najia1979]

Great video! cant thank enough.

[@kadeguidry3784]

The Creeeeeeeeeeeee-LoxP Recombinase.

[@pratikasiwatch8257]

precise explanation

[@SHEneedsAsmile]

Thanks for this!

[@ravikanthreddypunuru4199]

Cre recombinase creates recombination. Never thought in this way 🙄

[@ahmeter7538]

Thanks for this video, sir. How can I do this question. It seemed like a tough question to me and I couldn't. Can you help me? 1- Design a single construct, which results in labeling of - Cre (-) cells (that do not express Cre recombinase) with green fluorescent protein and - Cre (+) cells (that express Cre recombinase) with red fluorescent protein.

[@hoodyk7342]

Accidental click... i guess ill become a genetic scientist lol

[@nataliechapkis3988]

Thank you!

[@PunjabiMafia187]

excellent

[@erayulasbozkurt1241]

Thanks !

[@scottchificken]

2:08 express CRE in cells

[@zarkaroxane7345]

Creating an absolute knockout of the gene does not allow for research of genes ?

[@blinkeado69]

thanks!

[@chasecolin22]

Good video but y'all gotta learn how to soundbalance

[@AbadGorilla]

thanx

[@TALKyLIKEENGLISH]

❤

[@onibon1990]

too fast!!.

[@nl2457]

Then play it slower

[@skylarlane7857]

terrible explanation....

[@thankyou6555]

Wonderful explanation! Thank you.