Determination of Salt (as NaCl) in Food & Other Samples_A Complete Procedure (IS 3507-Mohr's Method)

[@melisapryce7370]

You are indeed a God sent. Wonderful video 😊😊

[@MicroChemsExperiments]

Thank you so much for your appreciations. Its a great pleasure for me.

[@Hakimmobariz-wu6jm]

​@@MicroChemsExperimentsHello, I am following the entire video procedure. When I add one milliliter of potassium chromate, it turns yellow and I titrate it with silver nitrite. No yellow color turns to red-orange. Please reply. It is urgent.

[@muhammadshahid9244]

I really appreciate your efforts and contributions. Thank you

[@shxnnaxo]

What are the controlled, manipulated and responding variables of this lab?

[@MicroChemsExperiments]

It's confidential.

[@shxnnaxo]

@@MicroChemsExperiments okay.

[@Rankun8]

In calculation part, you used 5.84. what is that? Molar mass of NACL is 58.44 right? what happened to 5.84??? Please tell me that . Thank you very much brother.

[@rhubarb6417]

Thats Molar mass of NaCl divided by molarity of Nacl

[@dilipsinh8416]

Excellent presentation Easy to understand Good job Keep it going on It's really helpful...

[@MicroChemsExperiments]

Thank you

[@annetorres1815]

may i know if this procedure can also be used in determining the salt in condiments like soysauce etc.

[@MicroChemsExperiments]

Yes. It's applicable for condiments.

[@corinalamkitnee5022]

@@MicroChemsExperiments the sample used in the video is in solid form. What if the sample is in solution form?

[@Nour_alqurneh]

can we use cheese or meat as the sample ? or the procedure will be different ?? and if NO , how can I determine the salt content in meat and cheese??

[@MicroChemsExperiments]

Yes. You can use this method for meat and cheese sample

[@Nour_alqurneh]

@@MicroChemsExperiments thanks alot 💖

[@yousraboukssessa4849]

can use the same method with cheese and meat products if so is the sample preparation method the same.

[@MicroChemsExperiments]

Yes. Same method with same sample preparation

[@yousraboukssessa4849]

@@MicroChemsExperiments thanks u very much

[@MicroChemsExperiments]

@@yousraboukssessa4849 you are welcome

[@gumuselsa5923]

hello, salt analysis in butter you spotted in the video?

[@MicroChemsExperiments]

No but you can analyze salt in butter following this video/method

[@jonaswong1287]

hi wonderful video but how do I find the concentration of cl- ion using the NaCl %?

[@MicroChemsExperiments]

We will make video later

[@joveen22]

may i know when this method used in analysis salt content of bouillon cube

[@MicroChemsExperiments]

Yes. You can use

[@adnanali-kt6ml]

awesome work but can i get the rancidity test of fat or oil

[@MicroChemsExperiments]

Adnan Ali, sure. We will work on Rancidity test soon. Stay with us.

[@jo-pr2zd]

Why did you use 5.84 as molar mass of NaCl instead of 5.844

[@MicroChemsExperiments]

You can use both

[@thanyaisri322]

What's the colour of the precipitate?

[@22UCHR026SELVAGURUK]

Why do you stop the titration the colour is not change in fully

[@shahadatmazumder6776]

Does this procedure apply to feed samples?

[@MicroChemsExperiments]

Yes

[@shahadatmazumder6776]

Thank you

[@kucingunpad3186]

Dear MIcrochem There's problem where if food sample pH is acid or base that sample doesn't change to reddish color, so i use NaHCO3 to make pH neutral, but do you have a clue how many NaHCO3 added? and if the sample is too darkish (for example soy sauce) how to determine the end of tiitration thank you

[@ciarakenny9428]

Can you use Potassium Dichromate?

[@MicroChemsExperiments]

No

[@msanthosh4980]

Is it applicable for all food products...??

[@MicroChemsExperiments]

Yes

[@usufqanoni753]

What was the source

[@rodriguefowe8106]

Hello. Can you give me the process to analyse salt content in butter? thank you

[@MicroChemsExperiments]

Same procedure can be followed for butter

[@jonathan14in1]

Hi, what happens if the color of the sample is brick red. How do we determine the end result of pale orange red color?

[@MicroChemsExperiments]

Sample color is brick red that doesn't mean that the total solution color is brick. Just focus on the liquid color rather than the sample

[@rodriguefowe8106]

And I also want to know the volume of potassium chromate that you need for the assay

[@MicroChemsExperiments]

Dissolve 5g potassium chromate in 100ml distilled water.

[@rodriguefowe8106]

@@MicroChemsExperiments ok. And now, how many ml do you use for dosage?

[@rodriguefowe8106]

How many ml for about 50 ml of d'amples that you prépare do you nerf for dosage?

[@EelasMusic]

Can you tell me how to extract the bacterial DNA from meat sample?

[@MicroChemsExperiments]

Eelam Music Production, we cant explain here and we dont have molecular lab.

[@EelasMusic]

@@MicroChemsExperiments just tell me the procedure,I mean the idea that's enough.

[@MicroChemsExperiments]

We dont work at Molecular lab. So we cant give you correct idea about it. Give us some time. We will get a moleculatar lab soon. Then we will study on it and after that we will try to provide what we found. Thank you.

[@EelasMusic]

@@MicroChemsExperiments ok thank you

[@sekinatsalau1527]

Can same procedure be followed for biscuits?...what is the nis standard for salt content in biscuit?

[@MicroChemsExperiments]

Yes. You can use this method for biscuit. Standard varies with different categories of biscuits and different countries

[@izyhew1081]

May i know, what is the sample used in this experiment?

[@MicroChemsExperiments]

Dry cake sample

[@josephanton6503]

I could use some help with the calculation and the 5.84 part. Here is what I did so far. 5.2mL x .1. This would give moles of titrant. The mole ratio of titrant to NaCl is 1:1, so then I multiplied by the molar mass of NaCl which was 58.44g. That should give grams of NaCl, but when I did it, I got 30.3888g. This would be way too high because then I would divide by the sample mass of 3.5028g and multiply by 100% to get percent. That would be over 867%, which is way off. Where is the 5.84 coming from and why did you not have to convert .867 to percent when you were done?

[@MicroChemsExperiments]

The calculation I have written in my video is correct as given in the Indian standard method (Reference is given in the title of this video).

[@benwelborn8828]

58.44g would be one mole of salt, indeed. And they also omitted units here - mol/L and ml. And they they got tired of multiplying the result by 100 to get a percentage... so they skipped dividing by 1000 and multiplying by 100. Instead they just moved the decimel in the numerator (of the molar mass of salt) one place to the left. Consider (0.0052L x 0.1mol/L x 58.44g/mol x 100%)/3.5g. All units accounted for.

[@alejandrotorres8586]

Thank you so much!!!

[@hamini1375]

Could you plz load a video for detecting starch in food as froud

[@MicroChemsExperiments]

Already uploaded

[@aswinvijay8085]

Will I get the same salt percentage if I increase the solvent quantity from 50 mL to 100mL??

[@MicroChemsExperiments]

Yes.

[@zeyadanwar7863]

what is the equation of the chemical reaction occured?

[@MicroChemsExperiments]

In future, we will make videos for chemical reactions for each video. Stay with us.

[@lailachoudary2373]

Which sample is used here?

[@MicroChemsExperiments]

Dry cake

[@saqi8863]

How prepare 1N silver nitrate and standardized

[@MicroChemsExperiments]

Noted. We will make video later

[@vlthachunglura5583]

Dear MicroChems, I have two questions. 1. Can I use to determine the NaCl content of edible mushrooms using this methods. And is the sample weight 3.5-5g is ok with the mushrooms sample? 2. For the calculation part, is there any unit or percent only, how can we know about. For example, your result is 0.867 and this is the percent from the sample weight you are taking or out of 100% NaCl, the sample contain 0.867. Can you please elaborate for me.

[@MicroChemsExperiments]

Ans: 1. Same method and sample weight is applicable for mushroom sample. 2. Our result was the salt concentration in the sample is 0.867%. This means, in 100g sample, 0.867g salt is present. In different unit, salt concentration is 0.0867 g/100g.

[@vlthachunglura5583]

@@MicroChemsExperiments Thank you so much for giving your time. Thank you thank you

[@mytestlabsdnbhd2437]

@@MicroChemsExperiments you mean 0.0867 mg/100g right? in different unit

[@DSVP123]

Hi in this calculation part is this 58.4 or 5.84

[@msanthosh4980]

What is that 5.84..?

[@MicroChemsExperiments]

Calculation factor

[@msanthosh4980]

@@MicroChemsExperiments is this common for all food products..??

[@rhubarb6417]

​@@MicroChemsExperiments What kind of calculation factor? Pls explain

[@prasannakumar352]

Hello madam how to check salt content in raw shrimp ..

[@prasannakumar352]

I mean procedure

[@MicroChemsExperiments]

Same as the salt determination in food

[@gurumaan2448]

Can we multiply the weight of sample with equation

[@MicroChemsExperiments]

No. Just follow the calculation given in this video.

[@senalpha]

Isn't better to filter the sample, since NaCl is water soluble ? So you work with a clear solution. I think salt in foodstuff is better determined with Volhard titration, in acidic media. Often i search for NaCl in cheeses, and sometimes in milk as well. With grated cheese: 4 g sample + 10 ml NaOH 1 M few minutes then 10 ml HNO3 1:3 few minutes (you get rid of fats and proteins in the swollen spongy bulk of cheese lumps). Add water to 100 ml then filter (no need pump, paper is enough). Discard solid mass and take 50 ml of clear filtrate, then add 20 ml AgNO3 0,1. Swirl. Then a tiny little bit of KNO3 and heat till coagulation. Filter on paper then add indicator (I use the titrant of COD - iron ammonium sulphate 0,25 N - old out of normality and add H2O2 let decant and use the supernatant, it works if you don't want to buy the proper indicator). The indicator is 5 ml of the clear liquid (NOT the eventual precipitate of Fe III !!!) and I titrate with Thiocyanate 0,1 N (Na or K is indifferent) till from white precipitate turns red brick. Obviously you need a magnetic stirrer to appreciate the end poin. You have to do a blank in the same conditions. Calculation: millies of titrant of blank minus millies of titrant of sample times 0,585 (or 0,355 if you want to express in terms of cloride) all divided half the weight of the sample. It gives you the % of salt. If you need the % of salt in milk (often required for food sophistications) you can apply the same Volhard titration but you need to deproteinate the sample with Carrez reactives..

[@MicroChemsExperiments]

Yes. Filtration is better to observe the titration end point clearly. Thanks for sharing your method

[@sabbiralemon3459]

Plz show us how to determine calcium & phosphorus percentage in feed sample.

[@skzslovestay8210]

how do i solve for normality if i was given 5 mL of sample + 50 mL of water?

[@MicroChemsExperiments]

Normality should not be charged.

[@sachinswarnkar1131]

Thankyou Very much ma'am and sir... And please Make some video Testing of Tomato Ketchup and Mix pickle testing of salt and Acidity percentage. Please

[@MicroChemsExperiments]

You can do salt test by the uploaded method of any food sample. In furure we will upload acidity.

[@RKSAINI-wv6nt]

MPS TESTING REQUIRED. Please make sure video.

[@MicroChemsExperiments]

Please mention the full form of the test

[@Wardi_lovers]

I'm writing for video..... How to analys sugar percentage... Please 🙏🏻🙏🏻

[@MicroChemsExperiments]

Thank you. We will upload soon.

[@Wardi_lovers]

@@MicroChemsExperiments thanks 🙏🏻

[@MicroChemsExperiments]

It's pleasure

[@LabAnaysis1385]

Make video Calcium content in food

[@MicroChemsExperiments]

Noted

[@nonamesatriapininggit9420]

NaCL 100% why ??

[@marygracetecsonbue8420]

Does it make you feel sad that the love that you're lookin' for... Is the love that you had?

[@MicroChemsExperiments]

Please ask relevant questions. Thank you.

[@hamini1375]

Very nice and perfect

[@MicroChemsExperiments]

Thanks a lot 😊

[@sushrisubhasinibehera1297]

What is 5.84??

[@MicroChemsExperiments]

Factor of the formula given by Mohr's method.

[@sebastianbester9737]

@@MicroChemsExperiments is this not meant to be the Molar Mass of NaCl?

[@MicroChemsExperiments]

@@sebastianbester9737 Molar mass of NaCl is 58.44. So you can't say that it is a molar mass of NaCl. You can say, it is a calculation factor which is derived from the molar mass of NaCl.

[@sebastianbester9737]

@@MicroChemsExperiments Thanks. How is the factor derived from the molar mass?

[@MicroChemsExperiments]

@@sebastianbester9737 58.44 is devided by 10

[@vijayvishwakrma8223]

Mistk in buret reading

[@MicroChemsExperiments]

Plz do at your lab correctly

[@rahulhait5812]

How can (22.8-17.8)=5.2 ???😄😄,kindly check your calculation twice before share a video

[@MicroChemsExperiments]

We are sorry for our silly mistakes. We can't revise in most of the cases because of our work-load. We will try to follow your suggestion next time. Thank you so much for your correction.

[@p.k.953]

😅 MCE be like: humare yaha Aisa hi hota hai 😂

[@amangirkhan3604]

5.84 kaha sa aya

[@MicroChemsExperiments]

Calculation factor

[@ShubhamSharma-gv4hh]

Can you please guide me about pickle ?? Please send contact details.

[@ANKITSAINI-q3b]

Calcium test animal feed

[@MicroChemsExperiments]

Noted

[@ANKITSAINI-q3b]

Urea test animal feed

[@MicroChemsExperiments]

Noted