Vitamin-A, Vitamin-E & Vitamin-D Analysis Using HPLC_Part-1 (Sample Preparation)

[@StunnedWithMe]

Great work guys..the topics are really useful for food tech students... Thanks for sharing vdos..keep it up

[@MicroChemsExperiments]

Thank you so much. Stay with us.

[@aymenogkush]

Great ! Good work guys !!! you're the best. Please if it's possible, could you make a video about the analysis of Aflatoxins, Vitamin C, Sorbic acid.

[@MicroChemsExperiments]

Thank you. We will upload video for vitamin c, sorbic acid and aflatoxins very soon. Stay with us.

[@borbro9077]

Thank you for sharing knowledge 😉

[@MicroChemsExperiments]

Welcome. Stay with us.

[@amornratrat]

Thank you for uploading

[@abdullaalrifat2232]

What is the name of method reference please AOAC or ISO or in house method?

[@nahidaalam9718]

Can we use n-heptene instead n-hexane?

[@youtubeok9228]

Excellent 👌 work 👍👍 thanks for sharing... It is very useful... Pls upload how to find calibration curve ..

[@MicroChemsExperiments]

Noted

[@rajeshrajagopal9894]

Very Useful.. Thanks a lot..

[@MicroChemsExperiments]

Thanks for being with us.

[@Rohan_bittersweet]

Thank you for sharing your knowledge !!! Is it possible to upload a preparation of vit. D2 and D3 standards for HPLC analysis?

[@MicroChemsExperiments]

Same sample preparation for D3 & D2

[@cyriljoseph7314]

At the end, Vitamin A and Vitamin D will be a solid at room temperature. How do you distinguish the two?

[@riyanshi_negi320]

Nice thanks. Can you share the calculation for the percentage claim.

[@mihiarwannousse3952]

I have a lab experiment to do, and it includes using oranges and determining the vitamin c content in them. Would this procedure work on the liquid juice of the orange? Thank you so much.

[@MicroChemsExperiments]

Yes, absolutely

[@tejbhansingh4300]

Heyy... Can be use same method for Vitamin D2 analysis

[@sabinastach3850]

Hello, After the first addition of water, foam is formed, and the layers do not separate, where can the problem be?

[@MicroChemsExperiments]

Add extra ethanol to remove foam

[@junaidalimeeran]

Bravo!

[@MicroChemsExperiments]

Thanks

[@vimalmathewjosie437]

Hi, Is the same for Vitamin D analysis? I would like to know how the extraction for Vitamin D2 and D3 is done.

[@MicroChemsExperiments]

Same procedure

[@vimalmathewjosie437]

@@MicroChemsExperiments ok then thankyou. Is it possible to upload an instrumental session for D2 and D3.

[@mohammedmousa9540]

Always in distinction we are in the second part🌹🌹🌹🌹

[@MicroChemsExperiments]

Will upload soon

[@HacenBoughanem-hl3rt]

can we use it for dairy products

[@MicroChemsExperiments]

Yes. You can

[@meenub3852]

Thank you so much ma’am. I have a doubt. I am a PhD scholar so I will add reference for this protocol , so which reference should I give?

[@krish_krish354]

AOAC method reference

[@nicham1552]

Thank you for sharing your knowledge !!! and You can share a reference that you used in this method for me please

[@MicroChemsExperiments]

It is a laboratory generated method

[@jeevithakutty6937]

Super

[@MicroChemsExperiments]

Thanks

[@simantaneog7150]

could you please provide the reference for this procedure

[@AmeerHamza-i6c]

Please guide about Cooking oil

[@MicroChemsExperiments]

Same method

[@AmeerHamza-i6c]

@@MicroChemsExperimentsOK I try it

[@krish_krish354]

What sample taken for analysis

[@hind1245]

Thanks so much..💚💚

[@MicroChemsExperiments]

You are most welcome

[@titelmegu9632]

Please make videos for vit b1,b2 and b3 as well

[@MicroChemsExperiments]

Noted

[@titelmegu9632]

Please 🙏 make video on water soluble video

[@gauravkamble4085]

Is this process can applicable for liquid sample of Vitamin

[@MicroChemsExperiments]

Yes

[@gauravkamble4085]

@@MicroChemsExperiments can u please tell me about instrument condition for vitamin D .. please because my m pharmacy project is to find out vitamins content in juice and fortified it

[@natura599]

thank you so much

[@MicroChemsExperiments]

You're welcome!

[@zeyadanwar7863]

can you give us the equation of saponification reaction plz

[@MicroChemsExperiments]

We will make video later

[@StunnedWithMe]

Plz make one vedio on food sample analysis at industrial level.. Kind request🙏🏼🙏🏼

[@MicroChemsExperiments]

Which test parameter of food do you want?

[@StunnedWithMe]

@@MicroChemsExperiments i want to learn about sensory evaluation of food at industrial levels!

[@MicroChemsExperiments]

@@StunnedWithMe Taste, Odor are thr sensory evaluation. You can do it by yourself. Just take smell of the sample or take taste of that sample and write the observations in your reports.

[@krish_krish354]

Can you explain without condesner can we proceed this analysis will it work or not

[@durairajm8868]

Can not.all evaporate.ccondencer or long glass tube required air cooling condencer.

[@MicroChemsExperiments]

Yes. A condenser is must needed

[@nalinmedagoda6879]

Please help. We need to analyze vit B3(niacin) can we use this extraction protocol?

[@MicroChemsExperiments]

We are already working on Vitamin B3. Stay with us.

[@aswinkumar8016]

Can we take liquid and semi solid sample..... In the same method

[@MicroChemsExperiments]

Yes

[@mohdabrar2839]

THANKS

[@MicroChemsExperiments]

Welcome

[@deepasamnani7465]

Share gc calibration video plz

[@AmeerHamza-i6c]

How can we extract vitamin A , D and E in cooking Oil

[@MicroChemsExperiments]

Same method

[@chemmuhammad8471]

perfect What about the standard preparation and calibration curve?

[@MicroChemsExperiments]

We will upload another video (Part 2) for Vitamin A & E analysis. Stay with us for mobile phage, standard preparation and instrumental conditions.

[@chemmuhammad8471]

@@MicroChemsExperiments I can not wait Thank you for this wonderful work Please make video for vitamin D analysis Also

[@MicroChemsExperiments]

@@chemmuhammad8471 We will work for Vitamin D soon. Stay with us.

[@muhanad212]

can we use this method for vitamin D

[@MicroChemsExperiments]

Yes. This method is also applicable for Vitamin-D as it is also a fat soluble vitamin.

[@ramachandranr8102]

What if sample is in liquid like milk

[@MicroChemsExperiments]

Take 10 ml milk sample and do accordingly

[@LabAnaysis1385]

Make some videos using GC GLC machine plz

[@MicroChemsExperiments]

Which tests you want video for??

[@anjalinepal2849]

Hey could you please specify the official method that you followed?

[@MicroChemsExperiments]

It is a laboratory developed in-house method

[@totjungjung808]

L-ascobic acid ?

[@MicroChemsExperiments]

Yes. It is used as antioxidant during saponification

[@shrushtithakre]

What is the sample that you have taken?

[@MicroChemsExperiments]

Ground biscuit sample

[@ربي-ارحمني]

What is the name of this methode ?

[@MicroChemsExperiments]

Extraction with Saponification

[@ربي-ارحمني]

@@MicroChemsExperiments asque vous pouvez détailler svp

[@ashishguleria4742]

Can you share material of this experiment. Plzz.

[@MicroChemsExperiments]

We don't provide any document.

[@omarsayed2458]

What is the category food they are determined in?

[@omarsayed2458]

Where is refference?

[@omarsayed2458]

What is the limit of each vitamin which this method can determine?

[@MicroChemsExperiments]

Almost all kinds of food you can analyze with this method

[@MicroChemsExperiments]

No specific reference method is possible to say. It is in-house developed method. We took idea from AOAC method of retinol determination and other journals.

[@MicroChemsExperiments]

@@omarsayed2458 LOD 0.1ppm And LOQ 0.5ppm

[@vitamins9186]

How to prepare raw milk sample?

[@MicroChemsExperiments]

Sample precedure as described in this video.

[@aswinkumar8016]

Part 2

[@MicroChemsExperiments]

Please wait

[@muhammadyousaf-xj2cd]

Soap analysis

[@MicroChemsExperiments]

Which parameter?

[@zeyadanwar7863]

can you give us the equation of saponification reaction plz

[@zeyadanwar7863]

i mean the sample + ethanolo + l-ascorbic acid + potassium hydroxide, why are those used and what do they give out as a product?

[@MicroChemsExperiments]

We will make video later