DNA Replication (Prokaryote) TERMINATION - Ter sites and Tus protein together stop DNA replication

[@ateendradubey]

I think there is slight error at 4:52 …. β-cleft is not -vely charged but +vely charged as it is composed of 2 twisted anti parallel beta strands containing basic amino acids (positively charged amino acids)… this will allow it to bind and intercalate tightly into -vely charged ter sites’ DNA major groove (as u know DNA is -vely charged due to phosphate anion in its backbone)

[@theCrux]

Excellent catch! I will pin your comment so others can benefit from the correction :) Thanks!

[@ateendradubey]

You are probably the only channel to explain this in detailed yet simplified manner. Excellent video.

[@isamv0998]

You've helped a lot! I wish I had found your channel before, thanks😊

[@mytumin]

You are so good!!

[@diyabhatt9127]

Thankyou for the explanation

[@Jimmyi9v]

Why fork 1 cannot destabilize ter tus complex? (At 6:30)

[@matetskvitishvili8150]

Do you have a recommendation for a book to see all of these in a more advanced fashion? I mean to read all of these on my own. I have "Principle of Biochemistry" by Lehringer but it is very basic.

[@theCrux]

Lehninger is a great book! Unfortunately, a lot of my content is not derived from textbooks. I use literature reviews and articles to assimilate most of the information (especially if textbooks fall short). You can find all my references on Patreon, for all videos made after July 16, 2022 (link to my Patreon in description).

[@matetskvitishvili8150]

thank you@@theCrux

[@aliyyaatta7372]

Why cytosine flipped ?

[@theCrux]

Because the Arginine lines up the cytosine at 6th position and apparently only Cytosine is able to interact stably with the Arg-198; perhaps some sort of co-evolution?! The C-flipping and Arg-198 interaction allows a stronger/stable Tus-Ter complex. Flipping itself per se happens because of melting of the 15bp region and sampling/melting of the Ter site. Why it evolved that way, is a more of an unclear area to answer.

[@edthoreum7625]

👏👏👏