Double immunodiffusion (Ouchterlony Double Diffusion) (FL-Immuno/58)

[@shubhamb.deshmukh7920]

I find a mistake in the statement made at 3:19 min "If we get arc-shaped band..." because if the arc-shaped band is obtained it simply means that the two antigens have a set of identical epitope groups with respect to the antibodies used. Although the two antigens can differ in other epitope groups but the antiserum used may lack antibodies to those groups. So this arc pattern does not mean that the antigens are identical and doing so is wrong.

[@FrankLectures]

+Shubham B. Deshmukh thank you for pointing out the mistake. Yes, the correct statement should be... "If we get arc-shaped band, this means the given antigens have identical epitopes or antigenic determinants".

[@shubhamb.deshmukh7920]

Hey! Thanks for appreciating. Well, your video made me think better :)

[@FrankLectures]

thanks and hope for more feedback like this from you in future.

[@pamelamkhize285]

@@FrankLectures Hi there! I am a Lecturer at the University of KwaZulu-Natal. I have used some of your videos for educational purposes. Thank you for your excellent videos.

[@FrankLectures]

@@pamelamkhize285 Hi. Glad to know that these videos are helpful to you. Thanks you for appreciating my efforts. It really means a lot. 🙂

[@littleluck3527]

I didn't find your videos when I was taking Immunology class, but I find them when I'm preparing my certification exam. Thank you so much for those short, clear, direct videos. I like the logic and the steps that how you present these to students.

[@dikshitkapil4686]

This was really the first time i could understand this procedure. Thank you for making this topic so easy.

[@arghayeaye4591]

such a clear and time efficient explanation

[@t-alimichael3363]

OMGosh all these videos and finally a robot explained it best that makes sense.

[@husseinqasim4172]

I'm dr ruqaya from Iraq , thank you so much for excellent video 📹

[@shruti574]

Thank you Ma'am for your videos. They are soo good 💫. Stay healthy and happy 💫💫

[@icshahid]

To be honest before watching the video I had no idea of what DID was despite doing it in lab . But after watching o felt that I know everything about it. Bravo creater !

[@peaa666]

This channel is so freaking good. Illustrations are so good to understand. I wish the videos were in human voice. Keep up good work👍👍❤❤❤❤

[@knoshamaryam266]

very simply put and illustrated to understand , great work , thank you :)

[@FrankLectures]

Glad to know this. Thank you for your feedback.

[@cathg.956]

im so happy youtube exist nowadays with all this illustrations and graphics lol on my college days 12 yrs ago it is difficult to understand by just reading the book

[@wafaqa4236]

Very clear explanation for non-English speakers

[@FrankLectures]

Thank you for your valuable feedback.

[@sherilramos9167]

Thanks for sharing it! It was really helpful to look at this perspective

[@FrankLectures]

You are welcome :)

[@suhagroy5088]

THAT IS A VERY NICE VIDEO WHICH HELP ME TO UNDERSTAND THE TOPICS MORE EASYLY.JUST CARRY ON

[@sanjanagupta8372]

Great info ... Thank you

[@inna7610]

Thank you so much to save my quarantine semester :,v

[@albertamensah9048]

Thank u very much.....Really helpful

[@MohammadHQadir]

Thank you so much for such a useful video 🌹💜

[@FrankLectures]

Thank you for your feedback.

[@mattamounika2984]

Fantastic explanation

[@FrankLectures]

Thank you.

[@notanurse2373]

For the love of god, find someone to read your script who is human and can pronounce such complex words as "agar."

[@vinamratasharma7698]

😂

[@wanderer37]

For the love of god , find some gratitude in your heart 😊

[@jaydeepdumadiya2464]

Jordar video hto.... Love from gujarat , INDIA🔥🔥🔥

[@aaliabhatt5845]

Thanks loads... For the video... Helped me alot... 😊

[@FrankLectures]

Thank you for your feedback

[@manognakuchibhotha7018]

very informative video and clear explanation... thank you

[@mohitsinha1983]

Thank you so much

[@FrankLectures]

You're Welcome

[@chaimamouas218]

Great work thnk u 🇩🇿🇩🇿

[@superbscientist743]

For non-identical antigens (a and b), do we need to have information about their epitope... ? So that we can decide that which two antibodies to be used (as you said antibody A and antibody B to be put together).

[@amaniewallace7021]

This was helpful. Thanks!!!

[@FrankLectures]

Thanks

[@rohinayameen6961]

Thankyou so much, very easily explained ♥️

[@nhanguyenlt7225]

thank you, very comprehensive

[@FrankLectures]

Thank you for your feedback.

[@vikasraghuvanshi8551]

Nice way of explanation...

[@FrankLectures]

Thanks

[@vikasraghuvanshi8551]

Frank Lectures your welcome.

[@drrrajkumar262]

Wonderful explained ❤

[@nemanjatepac]

What is unknown Ag or Ab? What we want to find?

[@israchan4474]

Thank you 🌸

[@mustajabalam3794]

Great help plz make videos on QC of RID , Elisa ,IF , SPE ,and flow

[@yahyakhaled7597]

One confused point for me , is ab is specific to both non identical ag at same time !! And if that is true so how can we depend on precipitation test for diagnosis of ag or it's used only for determination of concentration and identification !!

[@superbscientist743]

Me too have same query....

[@vadoelz]

Hello, thank you for this lecture, but can you explain or list the equipment that need to use and also the reagents that will need to use? Thank you.

[@wonderful3841]

Tq so much. It helps me a lot.

[@somashishsaha]

Excellent

[@immortalspirit9778]

Thank u well explained

[@aldanarosenbrock6537]

Me encantó! Muchas gracias !!!

[@FrankLectures]

Thanks

[@jeremymoore145]

thank you!!!

[@bhajan4290]

Awesome!

[@josef9141]

thanks alot this was so helpful

[@FrankLectures]

Thanks

[@user-vg2nn1bu7b]

thank you soo much you helped me many times

[@FrankLectures]

Glad to know this. Thank you for your feedback.

[@sainirajni783]

Thnkuu so much for making video on this topic

[@FrankLectures]

Thanks

[@docshaheen6741]

Awesome video again,

[@FrankLectures]

Thanks

[@magdahassan8052]

Thanks❤

[@mansijogi460]

Thank you 😊

[@bornsupremacy8]

Thank you

[@maramnawaiseh8051]

Awesome.. go on♥

[@FrankLectures]

+Maram Nawaiseh Thank you.

[@dusk0135]

Perfect!

[@HaneenFaizalHaneenFaizal]

How can we use 2 antibodies in 2nd case, since we dont know whether the antigens are identical or not🙄 we are testing that no?

[@fatimao3li955]

Mercie

[@bluegirl8058]

I'm MLT student, thank you

[@FrankLectures]

+Blue Girl you're welcome.

[@viktorpirozi2546]

Many thanks for this video, it was very helpful to me.

[@FrankLectures]

+Viktor Pirozi thank you for your feedback.

[@dariocosemans9151]

Hi, Sorry for my Flemglish (English with some Flemish in it) :) Here I go :P Can someone help me with this question? What makes the diffusion possible? The difference in concentration of course, but does the form, mass or friction forces take part as well or are these factor negligible? For example: When doing a non identity, you have three gaps. In the first one you put epitope 1 , the second one you put epitope 2 and epitope 4 (2 different antigens). In the third gap you put, three different antibodies, respectively Ab1 Ab2 and Ab4. When getting a pattern you will obtain 3 lines that will cross. You have 2 lines that are closer to each other (one line is from Ag2-Ab2 and the other one is from Ag4-Ab4. But the question is which line is from which antigen-antibody interaction? In class the professor told us that the line that is most far away of the antigengap (epitope 2 and epitope 4 ) is the one of the antigen with the highest concentratie. But is this it, or can there be something to add at the profs explanations ?

[@FrankLectures]

What I have studied is that the rate of diffusion is affected by other factors also such as size of the molecules, the temperature, gel viscosity, amount of hydration. And regarding your second question, it is difficult to answer. Need to study more for this :)

[@wafaqa4236]

Thank u 👍 🌷

[@FrankLectures]

You're welcome

[@Girlinterrupted955]

Thanks 🙏🏻

[@rupalipawar2787]

Good!!!

[@rupalipawar2787]

Good

[@simrankaur2471]

Ty

[@mustajabalam3794]

Really helpful ...thanks

[@FrankLectures]

Thank you for your feedback

[@evaortega5631]

Hi, this this technique is not cuantitative??thanks

[@FrankLectures]

It is a quantitative technique.

[@stz1238]

thanku😊😊

[@FrankLectures]

You're Welcome.

[@user-bp4oi9hb3c]

Thnx

[@rouaalharby2987]

Tnx✔️

[@ibrahimfaisal5315]

How can i get the antigen in the test we used two antigen and one antibody. one of these antigens is control with the kit ok,, but the secined how i can get it?

[@rakshitapawar1242]

Apt explanation!

[@FrankLectures]

+Rakshita Pawar thank you for your feedback.

[@k.rajkumar8650]

Please tell us about Rocket immunoelectrophoresis

[@alyaae.h3533]

شكرا جزيلا

[@FrankLectures]

بكل سرور

[@thedolphinbro6668]

ty!!!

[@sarasettou6717]

Thank yoouuu❤

[@imenemezioud2460]

Thanks

[@FrankLectures]

You're Welcome

[@Dhanapraveen851]

Super

[@neemabisht9091]

I didn't understand the non identical immunodiffusion reason why they make cross sign

[@FrankLectures]

There are two different antibodies in the antibody well. e.g. antibody A and antibody B. Each of the two antigen wells contain different antigen. e.g. antigen a and antigen b. When immunodiffusion will occur. Antibody A will form immune complex with antigen a for which it is specific. Similarly, Antibody B will form immune complex with antigen b. They will form precipitation lines at their respective zone of equivalence. One precipitation line will be of immune complexes made up of antigen a and antibody A. And second precipitation line will be of immune complexes made up of antigen b and antibody B. So, these precipitation line formed are actually two different precipitation lines which appear to cross each other. To understand better imagine these two precipitation line having two different colours.

[@auxjosemexixi1089]

Tnq

[@habibinoor7349]

Thankoo so much

[@FrankLectures]

you're welcome.

[@habibinoor7349]

😊😊😊😊

[@rohanerohane9142]

Traduction français avec la description svp

[@norahhakami3647]

ياليت الصوت يتغير

[@yusra9428]

Very well explained but the voice is so annoying! Anyway thank u🤙🏽

[@mubeenajasif8775]

Mubeena

[@carlavargaszavaleta9419]

Excellent

[@FrankLectures]

Thanks

[@yahyakhaled7597]

One confused point for me , is ab is specific to both non identical ag at same time !! And if that is true so how can we depend on precipitation test for diagnosis of ag or it's used only for determination of concentration and identification !!