Part 28: HPLC Intrumentation | Chromatography

Chromatographic Techniques (Playlist)
• Part 1: Chromatography...
Modern Pharmaceutical Analytical Techniques (MPAT) M. Pharm. (1st Semester) Playlist
• Part 1: NMR - Introduc...
Pharmaceutical Analysis B. Pharm. (1st Semester) Playlist
• Part 1: Pharmaceutical...
Basics of Analytical Chemistry (Visual Book/Graphy)
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Books Written by Dr. Puspendra Kumar
A Textbook of Pharmaceutical Analysis - B. Pharmacy (1st Sem) by Dr. Puspendra Kumar. 👇
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A Practical Book of Pharmaceutical Analysis - B. Pharmacy (1st Sem) by Dr. Puspendra Kumar. 👇
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GPAT Cracker (Piyush Publications) by Dr. Sanjar Alam, Dr. Puspendra Kumar et. al. 👇
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HPLC Instrumentation
Solvent reservoir in HPLC
Degasser in HPLC
Mixing Valve in HPLC
HPLC Pump
Precolumn in HPLC
Guard Column in HPLC
Sample Injection in HPLC
Analytical Column in HPLC
Detectors in HPLC
Back Pressure in HPLC

[@pkclasses100]

Please Watch at 720p or 1080p for best resolution.

[@tradingdiary7232]

this is a great work bcz less of video related to pharma world is uploaded. u doing fortune work in this direction. thanks sir...

[@pkclasses100]

Thank You. Keep Watching and Share the Video Link with your friends and help us to spread the knowledge.

[@viralkatrodiya2515]

Sir Calibration Of HPLC ka video banao na

[@Mr.B_2k01]

This is the best video i have seen until now !! you are doing a great work sir !! being in pharma field makes us to be proud by seeing professors like you !! keep doing it sir !! 😇

[@bohemiatopic8389]

Sir Im very grateful to uh... I had an analytical chemistry paper today... The time was short I relied on your videos only and performed very well in exam. Sir you saved my future... I will tell this story to my children thanks you so much ☺️

[@pkclasses100]

Haha.... Keep studying. I guess you will share the videos with your friends and juniors. 😍😂

[@adityapant9050]

Sir your teaching method is too good and so simple.. Thanku for helping students like us.

[@MuskaraBhiDo]

Ur from which course??

[@shenynaxx2729]

Thank u so much sir!!

[@omnivorousdiet4766]

Happy Teacher's Day 🌸🌸 Sir

[@shaikamarbegum3318]

Tan q sir.I watched u r videos helped to me 👍👍👍 for preparation of my Exams . 🎉🎉🎉

[@aqsamaqsood1410]

Sir you are great👏 i have watched your videos and they helped me understand my course...thanks alot Sir🙌

[@mitorika]

Thank you for making good movies on KZbin all the time.Thank you very much for your continued support.

[@NehaYadav-es4ek]

Very well explained and make easy to understand hplc technique good explain sir 👍

[@pkclasses100]

Glad to know 👍🏻 Thank you for the appreciation. Keep studying and Share the Video Links with your friends and help us to spread the knowledge. ❤️💜💙💛💚

[@poornimakaushik7194]

A very good vedio Help me alot 😃😃

[@AnkitGaurluckas]

Great just amazing for the first time I was able to understand it . So nice and calm explanation . God bless you .

[@pkclasses100]

Ankit Gaur Thank You for your motivational words. Keep Watching and Share the Video Link with your friends and help us to spread the knowledge.

[@alishagonsalves6174]

Useful explanation...nice video

[@pkclasses100]

Thank you for the appreciation. Keep Watching and Share the Video Link with your friends and help us to spread the knowledge. 👍

[@Keepstudying-ex1vw]

Nice sir,,,your way of explanation is easy to understand

[@pkclasses100]

Sunny Sharma Thank You for the appreciation. Keep Watching and Share the Video Link with your friends and help us to spread the knowledge.

[@Keepstudying-ex1vw]

Dr. Puspendra Classes ,,Yes my dear

[@mohanadurgabhukya6206]

Tq sir tomorrow is my exam biological instrumentation it helped me in detail....

[@balakrishnanm1268]

very nice explanation. i learnt something from that, which is very helpfu for my chemsitry knowledge

[@manjithjaidev692]

Pooooojaa pooooja naik fav student of the year

[@RahulThakur-pq3vm]

The ratio of retention of two adjacent peaks is known as A. capacity factor B. Separation factor C. Retention factor D. HETP

[@pkclasses100]

C and D and is already discussed in my previous videos. Please watch complete playlist of Chromatography which contains 30 lectures. Remaining will be discussed soon. Thanks Keep watching.

[@RahulThakur-pq3vm]

Thanku sir

[@lakshmeekantyadav3535]

What is capacity factor

[@ramanjaneyulum4878]

b

[@yogasaikiransrikurmam6972]

nice work guru ji

[@tusharpatil2842]

Very well explained sir Thank you❤️🔥

[@chandanmondal3843]

Very usefull explanation where easily understanding points are described sir

[@valvhalanirmala92]

Hats off for your concern on students Sir!I request you to upload videos on method development and validation of hplc using the instrument. Also how will you identify an unknown compound in hplc practically Sir

[@muzmmilmulla7057]

Thank you so much sir

[@Anoo8090]

very nice explanation sir

[@pkclasses100]

Anoo8090 Thank You for the appreciation. Keep Watching and Share the Video Link with your friends and help us to spread the knowledge.

[@RahulThakur-pq3vm]

U didn't told about factors in chromatography like retention factor,separation factor, capacity factor ,hetp Make video on this topic please on these topic.

[@akashjagdale8609]

Nice explanation sir

[@vaibhavshinhrajput8170]

Great sir

[@binteabdul6107]

nice explanation thanks for upload sir

[@pkclasses100]

binte abdul Thank You for the appreciation. Keep Watching and Share the Video Link with your friends and help us to spread the knowledge.

[@rongerduniya1535]

Best explanation!

[@muhammadishaq290]

Thank so luch sir. Your lectures are too good

[@ringtoneboy2.082]

Sir you are really Awesome! ❤️

[@samaira8319]

Thank you so much it helped me alott😊

[@EnglishExcel2208]

Nice xplanation sir #EnglishExcel

[@kamalikanath3170]

Thank you sir for this helpful vedio.....

[@manjunathyadav2306]

Thank you so very much Sir .......

[@suryaupadhyay688]

thank you

[@pharmacyjobs156]

Good informative video...

[@pkclasses100]

Pharmacy Jobs Thank you for your kind words

[@akhilalakshmi496]

Thanks so much sir...for ur valuable information

[@pkclasses100]

Welcome!!!!!!! Keep Watching and Share the Video Link with your friends and help us to spread the knowledge.

[@munawariqbal1916]

Amazing sir 👌😍

[@kanherimanohar9849]

Sir what happens with excess gas which is removed out from degasser..??it disturbs the sillica mesh of analytical column..??

[@pkclasses100]

kanheri manohar It may disturb the uniform flow rate of mobile phase and may be entrapped in stationary phase.

[@veerashekartalari7900]

Good

[@manitomoney550]

Hi Sir i m instrumentation student....I m working sales and service engineer in HPLC and GC instrument... This is very useful or not for my future???? Please sir reply detailey... I m waiting for your reply sir

[@pkclasses100]

This field is good and growing day by day.

[@manitomoney550]

@@pkclasses100 Thanks for your reply sir....

[@vasanttembye8538]

Do you know USP L38 HPLC column, polymethylmethacrelate gel, how to dry, prepared slurry, pushing solvent?

[@sonikacheema9700]

Nice video sir

[@pkclasses100]

Sonika Cheema Thank You for the appreciation. Keep Watching and Share the Video Link with your friends and help us to spread the knowledge.

[@maheshswami5226]

What is retention time formula sir??

[@Vatsalsharmaofficial]

Low back pressur high back presure

[@shikhatallaiya5886]

thanku sir

[@aqsakhan926]

Hplc column and column packing ..how can write??

[@Smoothcurveup52]

Thank you so much sir 🙏 Best explaination Sir but es me HPLC pump kyo mixing valve ke pass hai wo to Solvent ke just bad hai naa Privous video me

[@aqsafaiz9527]

Sir Please make video on supercritical fluid chromatography.

[@reddyvinayyelipeddi7362]

Sir I have a question that A hplc columns Like ymc c18 X select c18 All the dimensions are same Partical size and all interactions are also same So why and how the selectivity differs

[@pkclasses100]

May be slightly change in the mobile phase.

[@reddyvinayyelipeddi7362]

@@pkclasses100 Regarding column only sir

[@reddyvinayyelipeddi7362]

@@pkclasses100 And thanks for replying me sir

[@pkclasses100]

@@reddyvinayyelipeddi7362 As per Van Deemter equation it depends upon many factors.

[@reddyvinayyelipeddi7362]

@@pkclasses100 Thank you sir

[@aishwaryakukreti8768]

Sir Can you please explain regarding Faraday cup detector

[@kamleshpanghal476]

Sir please electrodics k vedio bnana

[@firozahmmed5042]

no of tertiary pump??

[@purushothamreddy6618]

Sir please tell me how many types of columns are there in based on usp and based packing

[@sievnrbtsievnrbt]

Tq sir

[@Vatsalsharmaofficial]

Constant flow rate pump

[@maheshswami5226]

How can we calculate peak area in hplc??

[@pkclasses100]

Instrument will give that area.

[@maheshswami5226]

@@pkclasses100 sir I am asking formula??

[@devashishrane476]

Sir U didn't mention types of injectors like Rheodyne amd septum injectors, we would like if post video about that🙏🙏🙏

[@jainampatel1838]

Sir can you explain which types of pump and detectors are used in HPLC in detail

[@MithileshKumar-yg5fb]

can you try to explain the HPTLC instrumentation? or that is similar to TLC

[@pkclasses100]

Video lecture is already available for HPTLC... Please go through the Chromatography playlist available in our channel.

[@yogasaikiransrikurmam6972]

are you an instrumentation student

[@karasalasyamnagaraju5964]

Thank you sir

[@pkclasses100]

Welcome!!!!!!! Keep Watching and Share the Video Link with your friends and help us to spread the knowledge.

[@thejaswinikn8674]

Thank you so much sir Can you provide a nodes for this topic

[@Vatsalsharmaofficial]

PTFE 1.80inch

[@kaustubhbhartiya6521]

Sir is it a real columns ur showing in video

[@pkclasses100]

Yes that is real column costing around 30000 Rs.

[@kaustubhbhartiya6521]

@@pkclasses100 Thank u sir.u r doing a very great work for student . keep it up making such videos for us.Best wishes for u sir

[@sagarjain1376]

Ok sir

[@gohiljignesh6499]

🤗🤗🤗🤗

[@brijendrasingh6800]

Sir I want to you discuss about in hplc principal short me sir Mera 1feb ko interview h please sir

[@pkclasses100]

Already videos available hai please go through the playlist of Chromatography.

[@Vatsalsharmaofficial]

C18 column

[@snehalbhagwat9837]

Plz add supercritical liquid chromatography vdo

[@pkclasses100]

Snehal Bhagwat Definitely I will upload. Thanks

[@Vatsalsharmaofficial]

Detecktor

[@RahulThakur-pq3vm]

What is the reason for peak tailing in hplc

[@pkclasses100]

Overloading/ Concentrated Sample/ Saturated or Old Column/ Degradation of Sample during analysis/ Ionization of sample.

[@sanafatima8002]

Sir aap plz Hindi m explain krdejiye HPLC and gas chromatography

[@pkclasses100]

Dono ka animation available hai. Search karo HPLC Animation by Dr. Puspendra and GC Animation by Dr. Puspendra. Easily samjh me aa jayega.

[@sagarjain1376]

Sir..please upload your videos fast

[@pkclasses100]

Sagar Jain Thanks for your comment. I will try for that.

[@sagarjain1376]

Thnku sir... Sir plz upload ur pending chromatography and spectroscopy videos soonnnn...

[@pkclasses100]

Under Chromatography few small topics are remaining i.e. Size exclusion, ion exchange, affinity etc.

[@sagarjain1376]

Ok sir

[@sagarjain1376]

Please please please sir🙏🙏🙏... upload your spectroscopy videos ..pending uv visible..nmr..mass..plzzzz

[@bharathkumarbachalakura2128]

Sir im m pharmacy student pls upload organic chemistry topics electophlic and nucleophilic aliphatic and aromatic compounds

[@brannigandupreez2083]

You should try, THE ORGANIC CHEMISTRY TUTOR, on youtube.

[@pkclasses100]

Yes, they are uploading good lectures

[@Vatsalsharmaofficial]

Constan flow rate

[@Vatsalsharmaofficial]

10cm 25cm

[@Vatsalsharmaofficial]

4.6cm dia pre

[@Vatsalsharmaofficial]

1 ml per minut

[@dharamkaram7470]

Bhai hindi bolona

[@priyankadass7282]

Thank you sir 🙏

[@sivanagireddyvanga1131]

Thank u sir🎉

[@jyotishmitasaikia6125]

Thanks sir

[@pankajmodi3980]

thank u sir