Transcription Activator-Like Effector Nucleases (TALENs)

[@grace96342]

our college needs you as our professor, you explained something in 10 minutes which our lecturer failed to present in a 1h lecture. THANK YOU SO MUCH this helped me a lot

[@navadeepdorai2918]

Old standard of teaching... A gold standard 👍🏻

[@payalwankhede1393]

Finally I got it. This is the best explanation available on the internet. Thanks

[@g-host6527]

32 min left to study before test, this dude saves my life

[@susanavazquez224]

It is excelent explanation and so great it is done the old way of personal teaching, paper pencil and a great teacher! congrats!!

[@talimachet2419]

You have saved my life - I am eternally grateful. Thank you so much!!!!

[@md.golamazom2703]

Great explanation on TALEN I found on KZbin so far

[@monthappy2521]

Dude, I absolutely love you 💕 Greetings from Colombia.

[@MrPelafio]

I was just about to google difference between TALEN and ZFN, when at the very end you explained it. Wonderful!

[@lukapreloznik7123]

I'd give you a HEART, but only LIKEs are available on yt...very good explanation, thank you very much!!!

[@harryli2053]

Best explanation ever! Thanks so much for your clear explanations and visual demonstrations!

[@tereabentley5896]

Thanks, this is a great explanation. Xanthomonas FOK1

[@ozanatasoy5591]

Thank you friend. As a virologist it's very simple and informative explanation for me.

[@sancharan6342]

Again.. sir you are awesome.

[@angeloorsini1764]

The best one on youtube. Thanks man!

[@sarahbrm253]

the best* one ..

[@angeloorsini1764]

thx

[@mayso1104]

this is the best explanation I've seen. Thanks!

[@condimentado]

BEST EXPLANATION EVER!!!!

[@meriamtayar8982]

You are absolutely amazing. Great explanation! Thank you!

[@askarrafi243]

Thanks for your complete and clear explanation. It was awesome.

[@lend2150]

Thanks for this video. You simplified it well. May I correct that it is FokI or Fok1 (Fok - One not Fok L) and it is Xanthomonas (not Xanfhromonas, it is t not f).

[@gregorysmith1134]

Excellent explanation.

[@Nico14071997]

Take a shot every time he says "transcription activator like effectors"

[@houmamkitet9555]

Very nicely explained, thank you very much for making the video

[@jeminmangukiya8655]

bloody brilliant

[@aquamarine3699]

Thank you for the clear explanation!

[@albertobray6963]

AWSOME explanation!!! Thank you so much

[@paygeshelton6252]

Thank you so much!!! This was amazing and easy to follow!!!

[@MonsieurDepadieu]

Thanks, great explanation clear as Clearacil®

[@changachangueria]

WOW!! nice explanation!! TY so much.. great job

[@hoimebanerjee1066]

Nicely explained. Thanks 😊😊

[@mahachris5477]

Thanks , you made it look so simple.

[@mgold9999]

You could have mentioned the 34 aa repeats in the TALE where aa 12 and 13 are variable and specify the DNA base maybe.

[@misabella323]

Thank you sooo much!!

[@annakassis8175]

That's the best explanation ever, (Fok1)

[@chutimon.ceefang]

Thank you so much this helps me a lot.

[@joannamaw8920]

Thanku for this!

[@deblinachakraborty5231]

😊😊make more vedios sir, we are in need.

[@karamella565]

thank you very much.

[@JMmitchell633]

Great explanation....thanks!

[@harleyjerram9699]

Thank you, really nice video!

[@celinec6366]

Is it true that Fok l cuts 9 nucleotides downstream in the first strand and 13 nucleotides upstream in the other strand? I didn't understand it from your movie.

[@paucska]

very good explanation, thanks! Although: what are the chances these sequences getting attached to the wrong place? :O I mean there are some chances, aren't there, considering the very lengthy and extensive size of the human genome...

[@sudiptasarma]

So, on the paper, it says that RVDs of TAL effectors correspond directly to the nucleotides in their target sites, one RVD to one nucleotide..my question is TALeffector are amino acids only, so how can one amino acid recognize one nucleotide? it is generally, three nucleotides represent one amino acid..or correct me if I am wrong please, bit confusing

[@pauladalcinmartins7421]

Great explanation!

[@christenemelad5435]

Excellent work

[@ChengVincentckf]

Great explanation, I am not sure that is FokL or Fok I

[@ardalanmansouri8176]

It is actually Fok1 (or capital i as a Greek number instead of "1"). For more information regarding its discovery please go to the following link published by the first researcher discovered it: www.ncbi.nlm.nih.gov/pubmed/6282705

[@ayaqz3144]

thank you

[@sapastje]

helped a lot, thx!

[@XamaL1]

Thank you !

[@Hansel551]

Nice.

[@ally850]

skip to 2:56, first few mins are bogus

[@serventofalfaandtheomega3040]

Thanks

[@Neo-rd7ef]

Thank you very much

[@Christina-eq8yc]

THANKS!

[@gasperkosmac7672]

THANK YOU

[@Samsu7777777]

Ultrasonic lecture

[@aurberdyson1940]

Uh... I'm curious, but not professional. I've seem many books write Fok"I"(I i) instead of Fok"l" (L l). So, could anybody tell me which is right? I'll be appreciated. :)

[@benjiprice8822]

are you talking about restriction enzymes or is it something completely different? please do answer me, how about you @ozan ATASOY

[@aronimitra28]

RVDs in a particular TALE recognizes different bases. So change in RVDs results in change in sequence specificity. 9 TALEs don't copolymerize. Only two TALEs with different RVDs bind to two adjacent sides of Fok1 recognition sequence. You're explanation is pretty much wrong.

[@nidhyajoseph1457]

Can you please elaborate? I did not get what exactly was stated wrong here.. Also how are both the TALEs and REN -eg. Fok 1 made so specific for a particular sequence? Both the TALES are different sequences right? Are there any chances of both these TAL effectors binding to each other..?

[@aronimitra28]

Nidhya Joseph TALES from Xanthomonas has a DNA binding domain and an activator domain which allows it to regulate the activity of certain genes in it's host. The DNA binding domain has 30 repeats of 33-35 amino acid residues. Out of these 35 residues, residue number 12 and 13 are variable in the repeats and thus they are called repeat variable diresidue. These two amino acids are responsible for recognition of a single nucleotide in DNA. Amino acids NI at RVDs recognizes adenine, NG recognizes thymine, HD recognizes cytosine and NN recognizes adenine or guanine. So if you know the sequence of your DNA then you can design TALENS that bind to your specific sequence by changing the amino acids at 12 and 13th position of each repeat. In this way the talen can bind to your sequence and the DNA cleavage domain of Fok1 fused to the DNA binding domain of TALE can cleave the DNA. Generally two TALENS have to be coexpressed in a cell, one that binds upstream of the cleavage site and one that binds downstream. When the two will bind, two Fok1 domains come in vicinity and cleave the DNA molecule. The cleaved DNA can now be joined by non-homologous end joining which is an error prone double stranded break repair process , leading to incorporation of mutations in your desired sequence of interest.

[@056vatsalapandey3]

thank you booooooooooooooo

[@mariahfoley3645]

What about RVDs

[@dia6976]

talens by XANTHOMONAS BACTERIA

[@cannizzosam5417]

* FOK1 not FOKL

[@benjiehutchison177]

It's not FOKL, it's FOK1 The bacteria is also called Xanthomonas, not what was described in the video

[@eathaare5358]

nine-tails hah

[@metinhuneren2163]

I cant focus on video because of your pronunciation