Epinephrine Signaling Cascade - GPCR (G-Protein Coupled Receptor)

[@echoyang3546]

I have a biochemistry test 3 days after and you are a life saver!!! These moof uni videos must be sent by god to help me understand all these stuff~:D

[@MoofUniversity]

echo yang Hahaha! SWEEEEEEEET! I'm happy to have helped your out! I hope you rocked your exam! :D

[@MrAndreaCaso]

Congratulations. Excellent Videos. We are lucky to have people like you in this world ;) Keep it up!

[@MoofUniversity]

Andrea C. Wow! Thanks for the compliment! :]

[@moy2010]

Cool, definitely one of the best explanations on the net :D. Kudos for you! And a small petition for you. Could you please make a video aobut g-protein kinases and arresting funcition cascade? Thanks in advance :).

[@nicolebill6426]

Thanks for this video! Very well-organized and easy to follow!:)

[@MoofUniversity]

Sure thing! Thanks for watching!

[@rickanator65]

I love this! Thank you for making this video!!

[@MoofUniversity]

rickanator65 Sure thing, dude! I'm happy you love it!

[@awishfulthought]

Awesome Video Mahfoud! Really helped paint the whole picture on what this signal transduction pathway is used for. My professor's been focusing too much on the mechanism and chemical structures of these proteins that we don't know where the mechanism applies to!

[@Capojutsu]

This video helpful? Once again you are a great teacher. This video is genious!

[@santyguita]

Epinephrine, fight, and flight; how to get ATP (↑glycogenolysis - ↑gluconeogenesis) - relation with caffeine. GREAT VIDEO

[@jenniferwroblewski9700]

I have a test on this on friday. I love you, dude.

[@gerardo2582]

Great video! Makes complete sense

[@MoofUniversity]

G Lopez Thanks! I'm glad, dude!

[@kby5099]

I know that it wouldnt make sense but shouldnt epinephrine inhibit glycolysis by phosphorylating pfk2/fbpase2 enzyme. F26BP levels would be reduced and thats an activator of glycolysis. Its follows the same pathway as glucagon with the PKA so I assumed it would give the same results. Also the pyruvate kinase isozyme of livers can also be inactivated by phosphorylation. Glucagon would slow down glycolysis and activate glycogen breakdown. I can just use my logic to tell what they do from their metabolic roles but dont understand the mechanism that makes the difference between these pka activators.

[@briannadavis2867]

These are so helpful!

[@stephenoconnor8932]

This is an awesome video man! Thanks for the help :)

[@monikayadav681]

very good lecture

[@sylartick88]

thank you. detailed and concise.

[@MoofUniversity]

jefferson feniquito Sure thing!

[@tamerawado4933]

it’s quite gnarly

[@chavarocks151]

Awesome video! Two questions: What protein hydrolyzes GTP to GDP (at the end)? And the phosphorylation of glycogen synthase and phosphorylase kinase are taking place both in the liver AND muscle cells? (Because in the liver cell we want glucose being made but we only want it going through glycolysis in the muscle cell.. is that correct?)(obviously in this case specifically)

[@MoofUniversity]

+Chava First question: I believe the alpha subunit of the G-Protein has GTPase activity. So, it would hydrolyze the GTP to GDP. Second question: Yes. Epinephrine would want glycogen to be broken down in both the liver AND the muscle cells. The difference is that the liver will dump the glucose molecules into the blood to be sent to the muscles, while the muscles will use the glucose (or G6P, rather) itself in glycolysis. I hope that helps!

[@chavarocks151]

+Moof University Right. Okay thank you so much! I am so happy I fell upon your videos they are very helpful!!

[@MoofUniversity]

+Chava Sure thing! I'm glad you've found them helpful! :]

[@fgeng3828]

+Chava Beside the hydrolyse activity of the alpha subunit it-self, there are GTPase accelerating proteins (GAP) that speed up this process by stabilizing the transition state of the hydrolysis.

[@cbeaudette92]

Actually, 1 epinephrine molecule binding to the GPCR activates 100 G alpha subunits which leads to the creation of 100,000 cAMPs and activation of ~10,000 PKA enzymes then upwards of 10,000,000 phosphorylated glycogen phosphorylase enzymes!

[@czdaniel1]

Did The Teaching Company's "Biology: The Science of Life" send you here? I remember that (or very similar) sound bite being used, I think, in one of the cell signaling lectures when discussing epinephrine to emphasize the incredible efficiency of this hormone.

[@czdaniel1]

Ok found it. It may seem inefficient because it is not a direct path from membrane-receptor-outside-cell to GlycognPhosphrlase, BUT THAT long chain of protein interactions has the affect of amplifying the signal because once activated, every protein molecule involved in the chain takes time to turn off and so it keeps repeating it's role in the signal chain over and over with other protein molecules...until you end up with exponential growth and around 10,000,000 glycogen-phosphorolase enzymes. So every step in the indirect (long inefficient) protein chain inside the cell is what makes that single Epi molecule at the receptor outside so damn efficient.

[@donnaozgur5761]

Very helpful, thank you! :)

[@Lior621]

Best explanation I've seen! thank you!

[@slaytlarslaytlar6864]

before i have seen this video i was thinking that i wont be able to learn the cascade. thanks for it!

[@innasuero9035]

THANK YOU

[@MoofUniversity]

Inna Suero YOU'RE WELCOME, BUT PLEASE NO YELLING! Lol.

[@AmirAbuleil92]

Nice to see someones replies to the comments in his channel ,, keep it up

[@MoofUniversity]

TheSeeker Lol. Thanks! It's not always easy, but I try.

[@zk.a__lift]

thankkkkk you this helped a lottt

[@yannalinagongoraguerra4717]

Thank you very much. It has helped clear up so many doubts about this topic. May the Lord Jesus keep giving you wisdom and bless you always.

[@champakalakshmi6551]

Is audio book available with one digital display image ... I would like to listen

[@champakalakshmi6551]

I would really appreciate it

[@kimtaymartinez]

Thank you so much for doing this video! It was definitely very helpful.

[@tunneltrance]

great video, and awesome explanation!

[@MoofUniversity]

Thanks!

[@mony4119]

thank you so much you helped alot , shout out from egypt❤️

[@Aud-Rey38]

This video is awesome! Thank you!

[@MoofUniversity]

Singette à roulettes Sure thing, and thank you!

[@katiemcnall7454]

THIS IS AMAZING

[@sindrekristianalvsvag9976]

Totally helpful! Thanks :)

[@diankilee9004]

Thank for the helpful vid!!!

[@CalebKam]

What program did you use to draw this?

[@justindenson7560]

You sound like Corey Feldman.

[@MoofUniversity]

Justin denson Haha. I've never heard that before, and before Googling him just a moment ago, I didn't know who you were talking about. Is sounding like him a good or bad thing? Lol.

[@justindenson7560]

No, not a bad thing. Just an observation.

[@MoofUniversity]

Justin denson Lol. Cool.

[@carolinagondra1338]

I thought he sounded more like Dr. House. Lol. Great videos. Thanks.