Freezing of Adherent Cells

[@gr0meer]

That wasn't me, but I am surprised they did't check if they got single cells. Another thing is they didn't tell how to freeze cells: slowly (+4 -> -20 -> -80 -> liquid nitrogen) or immediately?

[@nargesmohammad3884]

How much freez media should we add?also if too.much of the freez media is added would it kill the cells? Please respond back. Thank you

[@kosheeka]

While adding a slightly excessive amount of freezing media might not be detrimental, there are some potential downsides: • Dilution: Excessive media can dilute the cell concentration in the final cryopreserved vial. This might affect cell viability after thawing. • Slower Cooling: A larger volume of media might take longer to cool down during the freezing process. Slower cooling rates can be less efficient for cryopreservation.

[@ukGunther]

i love your movies! Please keep it still updated :)

[@Dhanalakshmi-zr9pi]

did you filtered the freezing medium ?

[@eleanorsmith9706]

Can DMSO be frozen and have same benefits when unthawed ?

[@zillionz]

unthawed... so you mean frozen?

[@kosheeka]

Yes, DMSO can be frozen and retain its benefits when thawed under the proper conditions.

[@zillionz]

Who clicked unlike and can you explain why? I didn't see anything wrong here...

[@peggyfitzgerald1663]

Sorry hit the wrong b

[@breadfan262]

You never froze them.