3-part streak explained - spore germination and breeding mushrooms the easy way

This is a 3 week update on our 2022 breeding project starting some new mushroom varieties from spores! Check out our playlist “Breeding mushrooms from spores” for more of an in-depth overview of our process.
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[@crazyhank99]

Gary, I've watched a bunch of your videos now and I gotta say, you really rock. Love the work you do and the way you spell it out. I'm learning tons from you. Can't say "thank you" enough!

[@HarmonyHollowApiary]

Thank you SO much for this video! It is SO very appreciated to see some isolation technique - with the 3-section dish isolations and spreading. This has opened a whole new realm (for me) for cleanup and the methods behind it.

[@dhaval4570]

Its crazy how you manage large quantities of cultures, props to you Garry

[@FreshfromtheFarmFungi]

It’s like taking care of babies hah it’s fascinating! Can’t wait to see the results next 👍

[@julescircuits845]

This is what happens when a microbiologist falls in love with the mushroom kingdom, you get insanely professional level mycology!!! This content boosts my myco game big time! Cheers for making your talent available for us to learn from Gary :)

[@FreshfromtheFarmFungi]

thanks for watching and following along! I mostly just like making videos ha but I guess my knowledge and interests are unique which is why it’s so fun 🙏🏻🍄❤️

[@vortextube]

Best mushroom videos on KZbin.

[@chrgore]

thanks Gary! I finally did it with your jedi wisdom and knowledge. My mushroom farmer dreams are about to be realized

[@FreshfromtheFarmFungi]

awesome! Thanks for watching and goodluck with your new venture! 🍄❤️

[@Crazy_Terryy]

I’ve been searching for information on breeding and it’s hard to come by. Thank you for these videos Gary. Much appreciated!!

[@funghi-farm]

Thanks for this content, greets from Spain

[@peterschneider8080]

Thanks buddy your the best :D

[@bounceurabdelaziz4973]

Thanks very much Gary.

[@user-do7lp9fl8i]

Hay bro thanks for the lion's head and bear's head you shipped out this morning for me today. I'll be getting more varieties from you soon👍🍄

[@FreshfromtheFarmFungi]

awesome goodluck with your grow they are great mushrooms! 🍄❤️

[@MushroomFarmersGuild]

Love the raw hat dude.

[@johnriperti3127]

Very interesting technique. Thanks for sharing

[@chronicalcultivation]

It would be very interesting if you could try this 3 times with plates #2&3 using heavily diluted spore solutions. Maybe 20:1 and 100:1

[@filax4500]

Amigo, please do a playlist with these videos from the immaculate inoculation, that would be wonderfull

[@FreshfromtheFarmFungi]

they are on the playlist “breeding mushrooms from spores” but I will separate them out in the near future 👍

[@TheSaltPrincess]

Nice hat... You're great 💞

[@dhaval4570]

Garry have you looked up at studies that show double the yields by the help of electric pulsation and even increase of 0.25 times with help of thunderstorm music, I know it sounds wicked but idk.

[@you-fq7ug]

What up sensai

[@TayyabMohammadtay]

much love to you too love to listen the way you assemble your Mycelium literature

[@pineapplepileus8719]

Gary!👍

[@pineapplepileus8719]

I like the 3 part streak to dilute spore concentration my guy

[@donuts_are_good]

Dude where is that shirt?! I want one!!

[@FreshfromtheFarmFungi]

it’s on our ETSY etsy.com/shop/freshfungi

[@chairpotatoe]

How do you know the isolates you've picked out will be strong fruiters

[@FreshfromtheFarmFungi]

I don’t know until they are fruited

[@cyberbid]

When I try streaking on plates I get a white substance which is not mould or mycelium, it looks shiny and wet. Even get this from different vendors. Maybe it’s my agar? I buy pre made plates but might need to change that vendor also.

[@FreshfromtheFarmFungi]

it’s probably bacteria or yeast, try diluting the spores in sterile water until you separate out the colonies

[@cyberbid]

@@FreshfromtheFarmFungi Ok I will try this, I am struggling with this hobby at times but I have had some success with other strains. When you say dilute the spores in sterile water to separate the colonies, would it be best to do this on an empty Petri dish and what method would you use to do this?

[@zachsabe]

So Garry, thank you. I am wondering if when you isolate phenotypes on the agar if you have any idea of what the trait will look like? Or is it pure isolation and then finding out down the road what the trait expression looks like after the fruit has grown? Seems like a lot of luck per se and patience lol

[@FreshfromtheFarmFungi]

yes you can only go off of features of the mycelium - watch this video kzbin.info/www/bejne/opa4oZmppNOJrsU

[@introvertgenetics4055]

👍

[@qfudgedoggy]

Awesome stuff Gary! I have a spore syringe that I have tried to germinate on agar but had a lot of bacterial contamination. I will try to use this technique. Would you reccomend trying that on water agar or doing that on MEA agar dishes? I havent done any work with just plain water agar. I am just a beginner.

[@FreshfromtheFarmFungi]

try both - if you can get a clean plate on MEA it will grow stronger but water agar will be more selective for mushroom mycelium

[@chrisknasel23]

I am having trouble with getting spores to germinate actually I can't even get already grown mycelium to reproduce on my agar it just dies what is wrong?

[@FreshfromtheFarmFungi]

they may not be viable or if they are too dry might take a longer time than normal

[@codyprice6188]

Why don't you think they'll combine together? Doesn't it have a better chance of combining together due to the fact that their both oysters? Wouldn't it be more difficult combining two separate species?

[@FreshfromtheFarmFungi]

Im not sure, it’s just my hunch because they are from such different parts of the globe - I have been wrong before though and it sure would be an awesome hybrid!

[@codyprice6188]

@@FreshfromtheFarmFungi ok. I'm excited about this. Forgive me for my forgetfulness but isn't your rose bud oyster a hybrid? Were they from the same area?

[@FreshfromtheFarmFungi]

@@codyprice6188 rose bud oyster is a phenotype of our pink oyster strain - it is Pleurotus species yes but it is it's own species (Pleurotus djamor) and native to Florida and tropical regions. It was procured by mixing two pink oysters together. This proposed hybrid would be Pleurotus eryngii and Pleurotus citrinopileatus which is a bit further of a stretch 👍