Hyperbreeding (cordyceps) mushrooms: How to breed mushrooms with less petri dishes in shorter time

This video is a proof of concept for our new methodology of breeding mushrooms in vitro. Subscribe to our channel for more mycology videos like these!
MUSHLOVE
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[@MrDougmx7]

Keep up the great work and I really appreciate the shared knowledge! Mush Love, cheers!

[@cullsans]

Fantastic video, I am not digging into cultivating Cordyceps quite yet but it was really beneficial to get a specific look into your process from start to finish, including recipes and everything. Truly gold content that could easily be behind a pay wall, thank you for sharing this information with the world for free! Mush Love!

[@FreshfromtheFarmFungi]

thanks for watching! 🍄❤️

[@scotthogan6057]

Really cool, enjoyed the experiments immensely. Please explore the blue lighting spectrum for improved pinning further. Thank you! You've inspired my research in directions I can't describe.

[@slocoast5]

Right! Finally found what I’ve been looking for.

[@waynekaczmarek946]

No doubt. EXCELLENT

[@FallFluff]

Oh wow! Just found your channel but I'm loving it all! Looking forward to new vids

[@FreshfromtheFarmFungi]

thank you and welcome to the channel! 🍄❤️

[@phantomhawk01]

Gary is the Clark Kent of mushrooms 😎

[@ranjanty]

I don't know about the Clark Kent thing but I do know of all the KZbinrs on here he is the Carl Sagan of all the mushroom channels on here! He knows his stuff. I predict in 1yr 75k subs

[@LongDefiant]

Deep dive, I love it. Thank you so much!

[@LongDefiant]

shout out for the presto type PC'er. So many people go into debt getting the best when you can make do and buy the best when you are ready.

[@FreshfromtheFarmFungi]

thanks for watching! 🍄❤️

[@pacman3536]

What an awesome video. Very helpful, and I can’t imagine how much work that took to make

[@jaysonramsay3896]

This is very exciting content. Mycology magic in real time

[@LowEffortGardening]

Wowowow awesome video. I'd love to see that Agarikon bred for vigor. I'm going to try this with my maitake variety. Thanks for sharing your work

[@deco1976]

Your videos are great, great content 👍

[@Lexington-Felix]

l really love watching your videos ! Thank you so much. 🙂mush love❣✌🍄

[@MrDougmx7]

Great method and information, appreciate the video! Can you grow out master syringes on agar the same way for storage and expansion later? Cheers!

[@stevenlabonte2240]

Love the sound track! Who is it?

[@aidennymes6335]

I really appreciate your in depth videos! looking forward for other species you're going to hyperbreed. what type of cups are you using? they are autoclavable. what material are they made of? i wonder if they can be used with parafilm gas exchange holes for other species. thank you so much for sharing this!

[@FreshfromtheFarmFungi]

Thanks for watching! They are pp4 so autoclavable yes

[@steveklik7870]

Great work! Do the Cordyceps require any light for pinning? Maybe the presence of light is inhibiting them? Inspirational stuff.

[@FreshfromtheFarmFungi]

They do require light yes It appears the blue spectrum is better - Thanks! 🍄❤️

[@xspells]

Gary I love ur channel dude

[@waynekaczmarek946]

U R the best, no joke.. Thanks for the great video

[@niteshchaulaagain]

Love from Nepal💞

[@Dasha-dg3xf]

bro, you are the best!

[@ranjanty]

Gary, Is a black light the same as blue spectrum? It looks like a black light in the video .I'm getting ready to grow some Lions mane should I use a black light or a regular LED light? Thanks, love watching your videos . Oh in a comment to someone else on here he compared you to Clark Kent, but I suggested no more like a Carl Sagan of the mushroom KZbinrs.

[@FreshfromtheFarmFungi]

hey thanks I love Carl Sagan! the lights used are LEDs that you can control the specs on and are set to 50% blue/50% purple - UV is black light and is a different wavelength- it may still work but it is slightly different

[@alexfinn7989]

Really great video. But what is this Process supposed to do?

[@FreshfromtheFarmFungi]

it generates new strains/ reinvigorates the cordyceps - these are very susceptible to senescence so this process keeps them fresh

[@emrysmcwryn7902]

A mushroom that evolved to feed on the fleshy bodies of insects can be made to grow and fruit on cereal grain. How about you? Do you also grow on cereal grain?

[@ivanuruguay2011]

no air exchange in the lid for them?

[@henlomatt]

Hi Gary! Thanks for the video! One question, how often would you say should one start new from spore? I think you mentioned once a year, but other say every 4-5 years. Is that once a year for cordyceps only? Thanks again

[@FreshfromtheFarmFungi]

it depends on the species and strain, cordyceps degrades relatively quickly so more often for that one. Other strains can last years or even decades. I breed my weakest strains over winter

[@henlomatt]

@@FreshfromtheFarmFungi ok thanks for the insight! Have a great weekend

[@Mind69420]

Awesome technique

[@FreshfromtheFarmFungi]

thanks for watching! 🍄❤️

[@michaelmcnulty1875]

Have you ever considered using a mutagen like camphor to try and produce a gygas polyploid?

[@FreshfromtheFarmFungi]

no but I will look more into this! Thanks!

[@ladymyco8693]

Would that perhaps mean he could produce the sticky lids in advance of sterilization and the camphor mutagen in one step by possibly applying a light rub of an OTC camphor “salve” on the top(/bottom depending on perpsective) of the lids before sterilization and hope to promote even slightly more sterility and certainly control of the application of mature spore-beating fruit to the lids as well as producing the appropriate atmosphere(literally😆) of the camphor mutagen to possibly produce more, more frequent polyploidal crossings… I wonder!!! Off to reading more, however, Michael, I would love to hear insight from whatever your background is knowing clearly more about this subject!!!

[@Algumnomequalquer]

Awesome video

[@pacman3536]

Is is 79degrees too warm for cordyceps? What is the best temp for mycelium growth vs fruiting temps

[@FreshfromtheFarmFungi]

it would be cutting it close. Ideally I fruit mine around 63F and incubate around 72F - give or take a few degrees

[@raymond.duncan]

Vigorous.

[@icestriker777]

Why did you out the funnels over the pressure cooker regulators?

[@FreshfromtheFarmFungi]

to dampen the sound

[@louisw5858]

Gary, a small clarification that you said they senesce quickly ,does that apply to the dikaryotic mycelium or even the single ascospore mycelium. i mean im asking this cause say i have two spores that are producing a commercial strain then if the spores dont senesce then hopefully i can pair both of them whenever i want and hence use them forever.

[@FreshfromtheFarmFungi]

yes I think if they are stored on slants and kept cool out of the light it will last a long time - this is a very subjective topic and is strain dependent and species dependent, I will do a video

[@louisw5858]

@@FreshfromtheFarmFungi thanks gary, keep up your great work. we all appreciate it

[@tmhalula]

Assume you are using 79 F? Can you confirm ?

[@RicardoLeonardoAugusto]

I noticed you used the same forceps to manipulate all strains

[@CHIROTHECA]

band practice a bit loud? where to buy your cordy LC?

[@FreshfromtheFarmFungi]

we will have the next batch ready on ETSY soon it sold out quickly

[@bhavanijasti9372]

I heard cloning reduces rigorousness in cultures though they fruit so how you maintain your cultures good like your mmx3?

[@FreshfromtheFarmFungi]

I keep the progenitors on slants and start them again from spore when they start to degrade

[@bhavanijasti9372]

@@FreshfromtheFarmFungi woww thats great . mush love

[@compulsiveviewingmaterials]

do they taste ok, or just for supplement?

[@FreshfromtheFarmFungi]

they taste “mushroomy” if that makes sense ha I like the tea with peaches

[@logancanada1826]

What are those containers your making the cakes in

[@FreshfromtheFarmFungi]

these are deli containers (pp5 rated)

[@landen702]

Would this work for varieties that have low spore count that you cant spore print?

[@FreshfromtheFarmFungi]

yes just increase the time above the solution

[@louisw5858]

people say these species dont maintain viablility for long time then how did your mmx-3 that i bought still fruited. i mean you already seem to have cloned that mmx3 then how is it viable all these days? plz reply

[@FreshfromtheFarmFungi]

“long time” is very subjective. I just ran off this same stock 4 months later and got great results. It will degrade over time and this process will need to be repeated again.

[@louisw5858]

@@FreshfromtheFarmFungi ohhh that means the strain i received is a fresh one that you hyperbred again and hence the fruits look so good maybe 😅

[@spuzzum100]

Roughly what temperature do you cook the substrate liquid to?

[@FreshfromtheFarmFungi]

250F

[@spuzzum100]

Thanks

[@Chemoscopy]

did the clone of that multispore fruited out plz let me know

[@FreshfromtheFarmFungi]

yes it worked - so far about 5 out of 15-16 trials worked

[@Chemoscopy]

@@FreshfromtheFarmFungi amazing thats what i wanted to hear im trying the same. thanks man MUSHLOVE

[@zacharyswain7034]

Do you just spray the spent Petrie with alcohol before using again

[@FreshfromtheFarmFungi]

I usually wash them with my grain jars, let them air dry and spray with alcohol

[@Suntanned]

These videos are great, I only wish that the repetitive parts would be shown at 3x speed instead of just the jazz music and 1x speed. 🍄❤

[@FreshfromtheFarmFungi]

Thanks! I just wanted to film it entirely to try to make it in under an hour with no cuts on one take - I will be elaborating on this method in the future and will keep this in mind!

[@PurpleHaze2k9]

@@FreshfromtheFarmFungi I agree, though actually enjoyed it uncut at 1x. The music was my only detractor. I like that type of music, but I had to mute after some time. Keep doing long form videos please, because it's eye opening for a mycology novice like myself. I have so much theoretical knowledge but no practical knowledge or proper experience, and your videos, especially in long form, are the perfect bridge between that gap. Thanks and good work.

[@skyelord6229]

I thought I was at a Dead show at 30:00 😵‍💫.

[@jennak.8541]

holy mother of jupiter... that background music is A N N O Y I N G as F V C K!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

[@FreshfromtheFarmFungi]

this was me learning - we are doing much better audio these days 🙏🏻

[@mistersmith8962]

German ate

[@trnguyen1]

Zh

[@trudymackay7578]

Too much music and poor quality of the video I honestly think this guy was trying to transplant a blue glove with Vaseline

[@FreshfromtheFarmFungi]

I chose to film the process in it’s entirety for documentation purposes and the fact the whole process is just over an hour has set the bar to try to do it in less time for someone else. I wore gloves for sterility and the petroleum is to hold the mushrooms in place - thanks for the algorithm boost! MUSHLOVE 🍄❤️

[@heynowyouarearock]

@@FreshfromtheFarmFungi Algorithm Boost!

[@PluckMe]

Please skip music next time. Please