Gram Stain Procedure

I also have videos on the Acid-Fast Stain, Flagella Stain, Endospore Stain, Capsule Stain and more if you want to check them out! Here is a link to a playlist on all my videos relating to Microbiology Stains:
• How to Pass Your Micro...
LEARN ABOUT THE CHARACTERISTICS OF GRAM-POSITIVE AND GRAM-NEGATIVE CELL WALLS HERE: • Gram Positive and Gram...
Gram staining is possibly the single most important differential stain used to distinguish between different types of bacteria based on their chemical and physical properties. In this video, Biology Professor (Twitter: @DrWhitneyHolden) will cover the Gram stain procedure and discuss how it is applied to determine what type of bacterium is causing a given disease. Great for MCAT Biology Review! For more information on the structural differences between Gram-Positive and Gram-Negative Cell Walls, see Biology Professor's video: • Gram Positive and Gram... For more information on another type of differential stain, the Acid-Fast stain, see Biology Professor's video: • Acid-Fast Stain

[@jacquelinegaray8864]

explained better in 5 minutes than my 2 hour lab lol THANK YOUUUU

[@ronnetgross6789]

RIGHT!

[@heanna172]

She explains this really clear!

[@ProductofWit]

Burnt out teacher who might have been motivated during one point in his/her life. Everyone has seen those. It's always refreshing seeing motivated teachers.

[@victoraseigbu1635]

Better explained than all my days at Pathology block posting 👌🏾

[@BiologyProfessor]

Thanks :)

[@arnishagarwal2057]

it was really very helpful, for the first time I completely understood gram staining, thanks a lot 😃😃

[@bobbie2010]

Thank you for such a short concise explanation! It really simplified Gram staining for me

[@kcgamer7288]

Watching all your videos on bacteria the night before my lab test, Thank You a bunch 🙏

[@somanathshyamsundar685]

Thank you so much... You just summarised my 2 hours lab session in 5 minutes.. That too bet efficiently... Thank you

[@pmar2517]

Thanks🌹 Awsome explanation very much better than our college faculties

[@gto11520]

the best biology professor on youtube ! thank you

[@shahbazmalik3465]

Thanks mam your method of teaching is so good

[@tammyjohnson4439]

perfect explanation. easy to follow. thank you

[@BiologyProfessor]

I'm glad it was helpful! I have a lot of other microbiology videos at this playlist: tinyurl.com/BioProfMicro Check it out and like my videos. :) THANKS

[@nitishsolanki5306]

@@BiologyProfessor You explained gram stain very well

[@prateekchauhan6376]

ma'am thank you very very much... this was the most helpful vdo for me in 2018... keep making these cool stuff.. coz only we the students know how it feels when we dont understand anything...cramming remains the sole option but it is disastrous in the long term... 2 minutes ago i was getting frustated but i am so happy and calm ☺😏

[@anabuitrago7401]

Amazing simple explanation!! Saves so much time for study

[@Ujjwal93500]

Thank you mam aapke video se mujhe practical me bahut help hote hain

[@ghulammustafa-ik5xz]

thankio for this lecture beacause i know completly for gram staining

[@jeddulanas9262]

This helped thanks! I passed my lab exam! Thanks for sharing your knowledge!

[@BiologyProfessor]

That's great to hear! Glad I could help. :)

[@heanna172]

Congratulations! :)

[@priyanshiroshan3250]

Thank you doctor. You are a real life saviour before my practical examination

[@BiologyProfessor]

I hope you do very well and get a great grade!

[@priyanshiroshan3250]

Yup. It went smooth 😀

[@BiologyProfessor]

Good job 👏🏻

[@SafaraAshanti]

Thanks ! I appreciate you helped me big time before taking my exam tomorrow !!

[@BiologyProfessor]

Good luck on your exam! If you need to review Gram Positive vs Gram Negative bacteria cell walls before your exam, check out my video on that topic: kzbin.info/www/bejne/nnrNY5Zum7-Koq8

[@kashdashortsman8327]

Amazing explanation, really well laid out too! Thanks so much!

[@haleysanchez]

Thank you for this!!!! Awesome job explaining clearly what’s going on

[@swathim6930]

Thank u so much for this explanation

[@SherineYousery]

Thank you for the great explanation , helped a lot :)

[@avadheshkumarsinha685]

Your country name

[@aniketdeotale99]

Thank you so much mam...

[@shaguftamureed4637]

Good explained

[@joelexis8887]

Thank you so much this was so simple to understand

[@missjokeq]

my microbiology teacher is the worse. I understood this well, way better than in class. I over stained my bacteria because he was no help. thank you

[@timbougie2999]

Thank you! Best explanation I’ve heard

[@BiologyProfessor]

You are welcome 🤗

[@SatyaPrakash-xi8xt]

Very well explained. Good work.

[@maleeshamadubhashini2128]

it was very helpful. thank you so much

[@easycooking8570]

Thank you so much 💕

[@martintaleta9378]

thank you professor ur video is informative n ur so sweet!!

[@adarshapriyadarshansejpada7681]

Thank you very much ma'am for this beautiful lecture.

[@jamie32117]

ohhhhhhhhhh now i get it! Thank you sososososososososososososo much!!

[@Mimi-wy2es]

Thank you sooo much for a very clear explanation about gram stain! :)

[@muhammedshibas4997]

Thank you for your presice and perfect explanation.

[@BiologyProfessor]

Thanks! I also have videos on other stains if you are interested, like Acid Fast stain and Endospore stain. I also have a video on the differences between Gram-negative and Gram-positive bacteria, so view it if you get a chance. :)

[@Womentales]

such a clear explanation thankuuuu

[@alokagrawal2130]

MAM FIRST WE TAKE A SLIDE THEN WRITE N AND S ON BOTH SIDE THEN USE NORMAL SALINE AND SOLLEN DUE TO AIR THEN USE TO HIT THEN USE CRYSTAL VIOLET THEN USE IODINE AND SAFRINE

[@RRRRRRnBBBBBBBBB]

nice, simple explanation. don't forget to underline 'Clostridium difficile'

[@naveedahmad8407]

good helpful lecture......and next time plz mechanism explain

[@vr4real775]

You are literally the best u know ...... I just love your videos ..... U explain concepts clearly and 5-6 minutes ...... And trust me u clear the whole concept in one go..... ❤️❤️

[@BiologyProfessor]

Thank you so much 😀 I definitely work hard to keep things clear, concise, and interesting. :)

[@stinky8171]

thank you!!!

[@sagarikawrites5864]

Amazing! Thank you so much!

[@JonniStar251]

good explanation, it is of much aid for me. Thanks.

[@AsmaKhan-qu1vh]

Thanks for your help very nice video

[@prathapagiriharish2687]

It's a nice explanation mam

[@BiologyProfessor]

Thank you 😊

[@RiyaSingh-ib3os]

Thnxx mam... Nice explanation

[@hhv9668]

omg thank u for this explanation!!!

[@evolvan1]

Thank you!

[@kierrajohnson4508]

Thank you, very helpful!!

[@beatrizpassinho3919]

Wow that was perfect! Thank you so much!

[@BiologyProfessor]

Glad you liked it! Tell your classmates about my channel so they can learn too. :)

[@tes2143]

Thank you so much!!!

[@monisansari6457]

Ty

[@healthwisevibes]

Thank you so so much verey helpful

[@zoilonunez4178]

Great vid

[@BiologyProfessor]

Thanks!

[@babapanging8455]

Thank you mam

[@emanelsayed9178]

We wash slide after each step, right??

[@BiologyProfessor]

Generally yes

[@mohsinhussain9717]

Thanks mam😍😘😘😘

[@MardinUzeri]

Wow thanks!

[@abdourahmanbah8569]

very interesting!!!

[@coolstuff8868]

Thank u so much 😍❤

[@zahraiqba765]

Ur the best!

[@walikhan9019]

Nice explanation love from Pakistan

[@BiologyProfessor]

Thanks for watching :)

[@SoulReviving786]

Ma'am very good

[@SoulReviving786]

Ma'am what is your professional field

[@SoulReviving786]

I mean ...which field of biology

[@ikbalhussain1872]

Thank you madam

[@mkcmkc81]

thank you

[@sandisonkosi9835]

this is what i needed for my flow diagram always that lab assistance will ask us flow diagram before we do the practicals and am sure tomorrow i will be on the good books of heres thaaaaaaaaaank you and maybe i will score some point to my partner besides shez my crush

[@martinmcg6543]

very good

[@shivarajrawat999]

good 👍

[@loveisiah8]

Such a good break down. One question though, what is the purpose of the counterstain?

[@larsswig912]

To help see the non Gram positive bacteria better.

[@BiologyProfessor]

It is to see the gram NEGATIVE bacteria better. Since they won’t retain the crystal violet following destaining, you need a counter stain to make them visible.

[@suhaillone9837]

Wow awesome

[@BiologyProfessor]

Thank you!

[@Abdulrehman.M]

How you reached the whiteboard top to write the heading...(Gram staining procedure) May be you just used a stool or chair

[@theshaafi6611]

Thankz

[@tamiratbeshah1259]

Your smart

[@BiologyProfessor]

Thank you :)

[@farhanraza4524]

You have nice voice😍😘😘

[@divyamverma8392]

what is a mordant

[@BiologyProfessor]

a substance, typically an inorganic oxide, that combines with a dye or stain and thereby fixes it in a material.

[@aussiegordon847]

If you accidentally used saffrinin instead of crystal violet but then did all the rest of the steps would they all just look pink? Or does the thick cell wall only bind to the crystal violet?

[@BiologyProfessor]

they would all look pink

[@peterbadalamenti8240]

god bless you

[@parangelmallillin6065]

What will happen if you used the decolorizing solution first instead of the primary stain?

[@BiologyProfessor]

If you swap the order, the decolorizing solution won’t remove the crystal violet from the Gram-negative cells, and the saffranin will not be visible. All cells (Gram-pos and Gram-neg) will be purple.

[@chathuanushki6331]

Whats the purpose of decolorizing in gram stain

[@BiologyProfessor]

Decolorizing is practically the most important step (obviously, all are necessary) because the decolorizing step is where you get the differences between cell types. After the crystal violet and iodine, ALL cells are purple. The alcohol decolorizing solution removes the purple from Gram-negative cells, but not Gram-positive cells (unless it’s on too long). Then saffranin is used as the counter stain to make the Gram-negative cells visible so you can see them under a microscope. The saffranin would not show up on the Gram-negative cells if they were still purple because you had skipped decolorizing.

[@zaidalhajjaj6624]

Thank you for the informative explanation. Could you please explain in the last foto why are the bacteria aggregated in certain areas on the slide? was that only random distribution or there is a certain reason for that? Thanks

[@BiologyProfessor]

+zaid alhajaj It is a random distribution created when the bacteria were smeared across the slide and fixed to it for staining.

[@fernandoaseo7614]

shouldn't cocci(circle shaped) be the violet one, while the bacilli (rod shaped) be the pink one?

[@BiologyProfessor]

No. The cells in my video are generic examples. You are thinking of E. coli as a pink bacillus and S. aureus as a purple coccus because these are common examples. But there are many Gram-negative (pink) cocci like species of Neisseria (diplococcus) and Haemophilus (coccobacillus). And there are many Gram-positive (purple) bacilli like species of Listeria and Clostridium (both rods). In other words, cocci and bacilli can stain either color, depending on the species.

[@karenopt]

What happens if you forget the mordant or decolorizer for gram positive and gram negative?

[@BiologyProfessor]

It you forget the mordant, the crystal violet dye won’t stick during the decolorization and everything will end up pink from the safranin. If you forget the decolorizer, everything will end up purple because the crystal violet will be retained in both cell types.

[@serinmarypr7198]

so if I was asked that which is the gram stain should I say crystal violet or saffranin?

[@BiologyProfessor]

Technically, BOTH are required because it is a differential stain...one is used to show Gram-positive bacteria and one is used to show Gram-negative bacteria. If you want to get picky, you might be able to argue that the crystal violet is the main stain and the safranin is just the counter stain.

[@crisenciopaner5065]

is there an automated gram staining apparatus?

[@BiologyProfessor]

Good question. I’ve never seen one, but that doesn’t mean they don’t exist, perhaps in some really high tech lab somewhere.

[@isaacnudanu19]

and she's so pretty😍🥰...

[@filmholic862]

❤️

[@rahulkesharwani1200]

👍

[@Deepak-lq8xt]

👍👍

[@Deepak-lq8xt]

Maam u liked after two month😇😇 but ur video was awesome

[@BiologyProfessor]

Thanks :)

[@OVERDOSE]

Cute, Knows way more bacteria scientific name than me, likes to tutor, and her hair is perfect, well.. I'm in love :|

[@arundhatisharma5743]

are you still in love

[@suggestionkhanal643]

😃

[@johncgibson4720]

The name Safranin really sounds blue-purple because of sapphire blue stone. And the crystal violet just sounds like some pink illicit pills. So, this thing is really messing up our brain.

[@caribaez5711]

I did my gram stain wrong 😔💔 oh Jesus

[@BiologyProfessor]

better luck next time... ;)

[@djinthehouse]

is the channel free

[@BiologyProfessor]

Yes, you can watch all my biology and chemistry videos for free! :)

[@matshayaxesibe6292]

Your voice is very down. And your videos are helpful. Please try to raise you tone next time. Thank you

[@sohailkazi9703]

You're so awesome and gorgeous

[@agnibhamajumder469]

Ur are so lovely

[@Josh-tk3ip]

no YOU're lovely

[@hasha9]

will you marry me?

[@devisdecember3569]

stop lying ussssssssssss//////

[@BiologyProfessor]

...?

[@santoshsubedi466]

thanks you so much !

[@ibrahimakkari9384]

Thank you !

[@BiologyProfessor]

I'm glad it was helpful! I have a lot of other microbiology videos at this playlist: tinyurl.com/BioProfMicro Check it out and like my videos. :) THANKS

[@TravelMusicGuy8]

thank you so much mam

[@EdskillsNetwork]

👍👍