High Performance Liquid Chromatography HPLC

[@EDUARDO12348]

You tube University does it again... thank you

[@prashantchaturvedi5426]

Very informative, simple and clear..specially for students..thanks for uploading

[@goodgamexxx]

You wouldn't know definitively without standards, but it's typical in HPLC methods that the first few minutes will be chemicals that passed through the HPLC column without absorbing to the column. Chances are they designed the HPLC method so that happened. The 'other ingredients' are usually called 'excipients' in the pharmaceutical industry, and they may be proprietary mixtures of stabilizers/preservatives/flavor additives/ etc...

[@belifk]

It's a quite beneficial video. Thanks to all contributors.

[@seadonkey1987]

@282sanjay Yes you can use HPLC to measure these. However, I work in a hospital and we measure T3, T4 TSH and calcitonin via immunoassay. Its a much cheaper, faster and more sensitive method. You can obtain results within 30minutes, compared to HPLC which takes atleast a few hours and more working time to extract

[@tgkillings]

British people are good at explaining things.

[@huenghiemthao7313]

hi everybody, i'm from Vietnam, my daughter want to research this HPLC video, but we can't catch the speed of the reader. Can anybody help me to give my daughter the subtitile to understand what this video talk about ? please help us ... many thanks

[@Shigren]

I use one of these machines everyday, and don't know much about them. (neither does any of the other employees tho) This was somewhat informative. I've been self learning chromatography ever since I started this job. Kinda hard to do tho, as I'm not a chemistry buff...

[@jagathivalluru9271]

Thank u so much for making this video I did not see hplc operates before this video

[@Naminator99]

Yes, but only in aid of the separation. Then you would do 1H-NMR/13C-NMR or mass spect for identification. cheers

[@GUARDADOOSCAR4]

thank you, is very academic for people that want learning HPLC

[@goodgamexxx]

Yes, for quantitation or identification.

[@PianoChick36]

Very simple explantion! Very helpful!

[@mahidajayaveersinh5013]

nice vidio this is very usefull for clear basics about hplc

[@goodgamexxx]

Acetonitrile is the primary NIST and IUPAC name, and I've never seen it bottled commercially other than Acetonitrile.

[@yodaydyxz1752]

Very so helpful, thank you so much!

[@ShaSowmya]

brillant work,very informative and thanks

[@jnielsen20]

@saurabhCherished I would suggest normal chromatographic columns, if the product is well known, an affinity column might be aqble to do the job.

[@Anadinolin]

Its a Jasco HPLC system, do a quick google search for "jasco HPLC" it should yeild some results

[@mastonsilalahi9602]

The video appears to be quite useful... thanx for that

[@ashokkaushal7570]

This is a brief but good lecture.

[@helpontheway7856]

Coooool can’t wait for tomm!

[@twtmei88]

Thanks! this really helped. I wonder how they use this along with MS.

[@craneywatch]

The presentation was great, and the lab work too. But there were some glitches. The chart didn't show perfectly defined peaks for the strong substance. Then, the needle of autosampler was not shielded.

[@NRGermany]

Thx, helps me for my test in 2 days :)Greetings from germany!

[@saurabhCherished]

@Yibitech So which technology is used for large quantities?

[@MrDirkles]

Brilliant video. Very clear.

[@IvanTorres-mz3ed]

can anyone explain why the more polar component came out first? is it because the stationary phase is non- polar?

[@weisu1427]

Ivan Torres you got it

[@SwanteRSk8]

the liquid phase is polar. h2o is a polar substance which means that things that are polar will more easily dissolve in it. that means that the more polar substances will have an easier time following the water. don't forget that in solutions like dissolves like.

[@veggiecat6695]

Ivan Torres Yup.

[@dumpykhan]

Ivan Torres yeah and affinity too

[@minhtrietnguyen2507]

exactly! Because the stationary phase is non-polar, the non-polar component had a greater affinity to it. In other words, it would spend more time being attracted to the stationary phase in comparison with the other component.

[@Haro131]

I need to determine residual sugars in the shake flask cultures. I need to prepare culture supernatant for analysis by HPLC. How can I do that?

[@A_Dopamine_Molecule]

The quality of those peaks, tho... I do LCMS data review and you have no idea how happy I get when I see perfect chromatography. If the peak is messed up I have to give it a makeover with the software and possibly reinject the whole damn batch. Not a fun time.

[@MogauF]

Thank you for this video.

[@jayasrikitchen4511]

oh god you simply opened the column?!?! is it recommend??? first time I could see the powder of silica from the coulmn...

[@deathempire70]

Can HPLC be used to extract the individual ingredients from the injected solutions?

[@SamBiddlevfx]

thnx helped a lot with my chem cswrk!

[@SubhadeepD]

very good illustration !!

[@781967kh1]

great for university students in the first and second grades

[@hsiatc]

Is there any recommendation for effective HSCCC (high speed counter current chromatography) ?

[@IamFreeRu]

What is the price of a unit like this?

[@alexeidobinson9115]

hey really great video guys, keep up the good work ladzzzzzzzzzz

[@twistertheman]

Very amazing video nice and simple , but why u didn't add the applications of the HPLC it would be of great important to those who use it in pharmaceutical industry ,,

[@jahkah6996]

This is what i do for work!

[@ppca0290]

Gud 4 u

[@A_Dopamine_Molecule]

Nice. Do you work with the machine itself or do you do data review?

[@wagnerobom]

Jah Kah me too. But this equipment is very old...

[@TheSmootherCriminal]

Great video! Thanks

[@MrBingkaikayu]

anybody know whre to buy this product?can gime me web site or company supply this product.thanks.

[@ckhoueiri]

what would a home set up cost?

[@Lima547]

Thanks for posting! Very Informative!

[@netaburnum1359]

very useful for proteomics studies.

[@jameswhite7350]

no i disagree all of what she said is wrong, she got her a and her bs mixed up

[@azzu911]

can HPLC be used for finding the chemical content of fuel oils (bio-diesel)....?????

[@selvas4129]

very good !!

[@hoquebio]

great video that we want

[@rohankamble6334]

really a nice one for d beginners

[@ragulkuzhandhaivel9665]

This are very interesting importance instruments

[@farahelaraby2795]

Thanks a lot ! very useful

[@khuptuaha]

thnks a lot. its really helpfull

[@alokrajwins]

its really great

[@Lima547]

Very very good! Thanks....

[@TheMathTutor2]

Excellent, very informative

[@amandamitchell5995]

Thank you great video

[@CartoBalow]

Thanks for the vid !

[@doratoth2143]

Well explained tutorial, thank you.

[@Blindedinchains1]

ethanenitrile that's the correct way of saying it acetonitrile is old terminology although many books are still using it unfortunately

[@mitulpatel6191]

Thanks for this video......good...

[@NatashaPereira1991]

Good explanation...

[@mangesh636]

good video....................thanks

[@JVAJ88]

ethane nitrile... also known as acetonitrile. If it says ACN on the bottle, why not use the more common name :) good video though.

[@TheRyanChang]

because British

[@petejones4808]

Because those who know - know? To those who don’t know, it really doesn’t make much difference.

[@kittithapa]

thank you. it was helpful

[@suprmoviebittu3206]

How to use in only q c

[@ShinyShoesTech]

Great video

[@sxcosssxs]

thank you very much.

[@irimfiaz951]

I have an essay ... hplc .. not sure how to start or structure it sooo much research out there 🙄

[@manupalace]

lifesaver

[@anh20020]

thanks for this video

[@Nanoscientist2]

amazing thank you

[@jayabhayjournal5975]

So nice

[@vydehi17]

thanks for uploading

[@remeshh8706]

Thanks ...

[@neetisharma8447]

thanku..

[@rabaksilo77]

gooooooooooood and very nice

[@tangn6083]

thank U ! That will help me :)

[@Jennie_cokies]

nice video,

[@9665851477]

awesome

[@muhammad922]

amazing

[@chuyunzhang278]

nice video

[@AlonsoRules]

manual injection, dang that's an old instrument

[@aido92]

***** 30 years ago, we used to have to pack our own columns!

[@AlonsoRules]

for each run?

[@KiyomiTakada]

I'm more concerned with consistency between column to column. lol.

[@ravikumarvakkalagadda2814]

Thanks good video

[@pumbagaming6265]

agreed, visuals very clear

[@sateeshmsc]

great

[@rw1123]

cool video, makes more sense..i think!

[@vishalshelke9805]

nice one

[@mithlesh7042]

10years ago 😯😶

[@zagaroooo]

bravo bravo score

[@appleseeds5603]

anyone can explain me why do we use UV spectra to get a peak in HPLC method. Even though there is other spectrum such as IR, X-ray,..etc. ?

[@royalsocietyofchemistry4447]

Hi, This is likely because, as implied in the video at 3:41, the light needs to be set at a wavelength that is absorbed by all the components to be separated.

[@romaahmetov5550]

super

[@نورس-و1غ]

Tomorrow we have exam in HPLC , chemical science Baghdad university by Dr.yahia 😍😂

[@divyaharjan3412]

Useful for a lvels

[@barmoulali]

good

[@akif963]

nice vedio

[@FfejTball]

In other words, if you have to ask...

[@busrakandemir537]

biri bunu türkçeye çevirse muhteşem olur :)

[@NatashaPereira1991]

My Professor told me its about $30,000

[@rave208]

70,000AU

[@karbharipagare8195]

Nice try ...i more study and more impressive poster prsentation...