How to Breed Cordyceps Part 2: Combining single ascospore isolates

[@ermagerd101]

Wow. That was some expert level stuff! Thanks for letting us look over your shoulder while you work! Please let me know when you have more MMX-3 ready to go, I just saw Renegade Mushrooms video. I liked when he weighs his harvest and you can hear the laugh in his voice at the results. Full credit to you, excellent (tedious) work!

[@codysaunders7348]

You've got fantastic videos man, love your work.

[@FreshfromtheFarmFungi]

Thanks for watching! We appreciate the love - MUSHLOVE

[@funghi-farm]

Do more of this content ! I love it ! You explained it really good !

[@nuahtransit5858]

Good job 👍

[@momsmushroomsjodyfoster5786]

I do the same thing with my cracked plates

[@erikiacopelli451]

get a tri pod itll help alot

[@putinfamily1567]

Hi sir, I have a question for you. What is the single ascospores of cordyceps? and what is the single conidia? How to distinguish them? Thank you!

[@FreshfromtheFarmFungi]

ascospores are the spores of ascomycetes (they are different from basidiomycetes because they produce spores differently) Conidia are cell structures that bud off the surface off the mycelium - they are an asexual form of reproduction. That’s very general but if you research more you will understand

[@DesertAlchemist]

I thought the whole point of isolating the ascospores was so that you can combine monokayotic hyphae from two different strains I didn’t think, combining ascospore isolates from the same stroma would yield in a new strain since it has the same DNA. Correct me if I am wrong.

[@FreshfromtheFarmFungi]

this is a correct line of thought - there will be less variance but it will make a new, stronger strain instead of letting that line die off into senescence. This will only work for so long which is why genetic variance is important.

[@DesertAlchemist]

@@FreshfromtheFarmFungi cool I was confused so I could do that a few times. I’m trying to combine #7 with albino and make a new variance. This helps a lot thanks.

[@mv1y3eb2qs4]

@@FreshfromtheFarmFungi Why would the new strain be stronger?

[@dena4366]

can you share the link to the video you mention at the begging about mating by the techniques PCR and electroforesis please! Thanks so much

[@FreshfromtheFarmFungi]

kzbin.info/www/bejne/jIvEfKSPhb1kqZo

[@dena4366]

@@FreshfromtheFarmFungi do you know which primers are used for mating amplification? do you sequence your amplifications and how do you decide the pairing between strains. thanks

[@Chemoscopy]

for how many days the isolated spores stay viable?

[@FreshfromtheFarmFungi]

it varies by variety - it’s a biological agent and every case is different

[@trevorholliday7020]

I don't think you're doing what you think you're doing. Luckily, your efforts still produce. If I remember my 10th grade biology correctly, the genetic information is contributed from two spores to create dikaryotic mycelium. Those fuzzy white patches on your agar is that very thing. When you take from where two blobs meet, you're not taking the baby of those two blobs. Rather, you're taking mycelium from two individuals and transferring those two individuals each time..... unless I'm completely wrong of course.

[@FreshfromtheFarmFungi]

Thanks for the input but this is how mushrooms breed and form genetic variants. They meet, the mycelium fuse to form dikaryotic mycelium. It happens in nature all the time and is easily replicated in vitro. I have bred dozens of new mushroom phenotypes utilizing this technique.

[@trevorholliday7020]

@@FreshfromtheFarmFungi So, you're suggesting that when two dikaryotic mycelial masses meet, they exchange genetic information and create a third, genetically different dikaryotic mass?

[@trevorholliday7020]

@@FreshfromtheFarmFungi hello? Those mycrlial madses are already dikaryotic. I don't think you're actually "breeding" anything by letting them mingle.

[@joshandrews6100]

A single spore will germinate into a monokaryotic colony first. Once it meets another monokaryon it can breed with do they become dikaryotic. Cultivators isolate single spore monokaryon colonies all the time for breeding hybrids.

[@marcbrettschneider6616]

Yes multiple dykariotic mycelium can fuse. This is referred to as anastomosis. I just dont understand why anyone would want to start with a handful of isolated spores. You are creating new strains by picking from a few dozen. Mass spore to one side of a plate, let it grow to the opposite side, get the tips of the most aggressive multi genetic mycelium and make broth. Broth to grain and ultimately fruit it. Then clone the fruits and grow out each cloned mycelium to a batch for observation of yield, consistency, colour, ect. This is the way i do it, this is the way Amycel does it. We want to select from the largest possible bank of genetics

[@svengabelbart586]

First!

[@Chiksic]

Could you add a link to part 1? I can't find it

[@FreshfromtheFarmFungi]

Breeding cordyceps militaris: What is the best method to breed cordyceps? Plus beetle updates

[@FreshfromtheFarmFungi]

kzbin.info/www/bejne/hIPQaWhvYtKdnq8

[@Chiksic]

Thank you so much! Your videos are really helpful!