Immaculate Inoculation FIXED: kicking off the 2022 mushroom breeding project (germinating mushrooms)

We are kicking off the Holidays with an in depth breeding project - tune in and follow along as I breed 13 different mushrooms utilizing my favorite techniques and tricks to help germinate spores - for more info check out freshfromthefarmfungi.com Visit our ETSY shop Freshfungi etsy.com/shop/freshfungi Check out our recommended lab gear on our amazon store www.amazon.com/shop/influence...

[@FreshfromtheFarmFungi]

The Stropharia spores germinated and were clean - the others not so much. 30% success spores to LC not that bad - the rest of the innoculations have produced healthy mycelium so far! Cannot wait to get fruits!

[@codyprice6188]

So glad your following through with this project! 😊🍄❤️

[@ThahnG413]

That's pretty impressive considering how dirty even clean spore prints are, and if you ever need a new liquid culture recipe to try I reccomend 500ml of water and 1/16th of a teaspoon of pilsen light malt extract 3.3lbs liquid. It only costs about 10 bucks and it is the best recipe I have used to date for lc, my lc is always thick and the whole jar becomes a cloud even with some mycelium growing on the surface if you don't break it up because of all the vigor. You know you did the recipe right if once the lc comes out of the pc it has no to low color change since the amount of nutrients in pilsen light is so high

[@LarsLarsen77]

Not only were you talking with the needles right in front of your face, you even took the syringes out of the sterile field several times to hold them up to the camera. You need to work on your sterile technique. There is water all over your flow hood. You understand that spilling shit all over the place isn't sterile technique, right? At the beginning of the video you waved your ungloved hand over your sterile field. WTF? Flow hoods aren't magic. You're fucking your shit up before you even start working!

[@brandyhamilton8944]

Update on the matsutake? I would really love to grow and try that 🍄

[@jayo1247]

You're doing great work. Thank you so much for documenting what you do and sharing it here. It means a lot.

[@fatstratcatdaddyo]

I love your enthusiasm for science and mycology, as well as being well versed in the binomial nomenclature of the various fungal species and strains. You're very professional, and it shows.

[@SubFlow22]

That flow hood makes my mushroom fruit.

[@bounceurabdelaziz4973]

Thank you very much Mr.Gary, for your sharing, I look forward to your great video. Good luck.

[@MajorPolySci]

YES! thank you. a lot of great information in this video! i’ll need to turn on the bell icon for notifications so i know when you are having a live stream!

[@teuth]

thanks so much for the re-upload!

[@hansulrichjohner2694]

Very good video! Sorry for the first comment, but I am an old Physicist and and thus by necessity a precision nerd!

[@drewwhitaker315]

Great video, Great Info... Enjoy your holiday. See ya next year!

[@LongDefiant]

Ooo, I wanted to watch this again. Much easier, Thanks!!!!

[@Sartorri4666]

After all the work I went through to get used to watching sideways. Gee thanks. Lol, sorry I had to. You are the best and this is the reason. You did not have to go back and fix this. The fact that you did shows a level of heart that most lack. Well done. I am forever a fan and subscriber. Mushlove.

[@FreshfromtheFarmFungi]

thanks! it was my ocd and too many complaints hah glad you enjoy our content! ❤️🍄

[@Sartorri4666]

@@FreshfromtheFarmFungi Confession. I left a complaint, then realized I'm an ass. You do a lot for the community. I came back and deleted the complaint and laid down sideways to watch. My neck is still a little sore, but not complaining. Just kidding I just listened to the audio. That is what was important. Thanks again for everything, and for noticing me.

[@bounceurabdelaziz4973]

Excellent work it's a pleasure to watch your videos, Thank you very much Gary for your sharing. Dieu te protège.

[@zachsonpub]

pretty cool having prints made on petris!

[@probiogr3863]

this fixed video is like a dejavu but without the shrooms high

[@jacobjamar]

People are just effing about the angle. Great video man!

[@cheeselord8153]

Can you make a video on the snake venom thing? It’s super interesting and unique, and would love for a more in detail explanation of it all

[@FreshfromtheFarmFungi]

I will put it on the list - my brief understanding is that it causes mutations and can therefore make new variants. Not very precise but maybe microwaving mycelium for 10 seconds here and there could also do this 🤷‍♂️

[@Robebabu]

Please explain the vaseline/jar spore printing technique.

[@ScottWConvid19]

Have you got a video or any articles on how you do a sterile spore print lifting out above, using Vaseline and a jar? I've never heard of that process

[@jcondron]

Do you have a video on collecting spore prints on petri dishes?

[@hansulrichjohner2694]

Good video, but 25 ml +10ml +10ml gives roughly a dilution of a factor of 2. If you want a factor of 10, put 36ml of water and 4ml of spore suspension (not solution!)

[@FreshfromtheFarmFungi]

thanks for the clarity and precision it makes sense

[@TayyabMohammadtay]

BE BLESSED MUCH LOVE TO YOU TOO

[@Mitzi712]

Happy Christmas! Do you use glass or plastic Petri dishes for your prints?

[@FreshfromtheFarmFungi]

Merry Christmas! I like to use spent plates whether plastic or glass, usually I take the cracked ones when I open a sleeve and set them aside until I find the other half in another pack - if that makes sense 😄

[@zeratul600]

why this was available only in 320p resolution?

[@Robebabu]

What brand of needles are you using?

[@TylerHanke-he5kx]

how does the vortex machine work? there is no magnet like a stir plate?

[@FreshfromtheFarmFungi]

@@TylerHanke-he5kx it just vibrates in a circle when pressure is applied - no magnets 👍

[@randywhitmore7517]

20 minutes in and even I get the feeling his wife is getting angry. I'll be back, mines mad I'm 20 minutes into this.

[@Robebabu]

Does the Polysorbate 20 come sterile, or does it need to be sterilized before adding to the spore solution?

[@FreshfromtheFarmFungi]

lab grade should be sterile cosmetic grade is close but if you are careful at keeping the stock clean it should be fine

[@mirandabotanical9676]

I’m trying to understand how serial dilutions help isolate the spores away from any contamination. Is the effectiveness based on the assumption that the spores overwhelmingly outnumber contaminants on the spore print dish that you’re starting with?

[@FreshfromtheFarmFungi]

it doesn’t have to outnumber contaminants, just separating all the cells until they are diluted enough will give you a chance that there would eventually be 1-2 colonies per plate - if you do enough plates then you should get some clean isolates. that’s worse case scenario

[@mirandabotanical9676]

@@FreshfromtheFarmFungi thanks, that makes sense for going to plates. How long can you expect a spore syringe, kept at room temperature, to stay usable?

[@greyvoice5378]

Could you tell me more of the snake venom and how that works? What type of snake venom would you use? Where do you buy it? Do you mix it in with the spore solution or the polypropylene? Thanks in advance

[@FreshfromtheFarmFungi]

It acts as a mutagen to cause mutations. I am not sure about the exact procedures but Ill do a video on it soon

[@squirlmy]

Did you do that video on snake venom. I think it's way beyond my ability, but, it's such a strange application of venom, and learning how it works via visual cues on a video medium sounds really cool. 😎

[@victorwitness7414]

I heard once that rattle snake venom produced in a hyperbaric chamber is used as a medication. I don't know for what though. Before the Flood of Noah there use to he a Seventh layer to the atmosphere. Creating about 2.5 times the atmospheric pressure. This creates more oxygen in the air allowing for bigger animals and plants. Evidently changing the toxic nature of snake venom also. Mushrooms??

[@scottchambers8489]

Can you show me the thing you are extracting sterile water out of?

[@FreshfromtheFarmFungi]

kzbin.infoTT4VITYr7UM?si=ue8UdawoG3ntnfom

[@dc9515]

Gary you've put spores straight into liquid culture? Spore prints are notoriously un-sterile. Fingers crossed.

[@FreshfromtheFarmFungi]

yes diluted spores, and spores collected in vitro which are sterile

[@dhaval4570]

garry I was planning to try the spore to straight LC, how much spores should I drop in 1 300 ml Jar? Considering I don't wanna use the whole print / save it for future uses, I use an inoculation loop.

[@FreshfromtheFarmFungi]

check out our video here - the king stropharia and cordyceps look healthy using this method kzbin.info/www/bejne/fZKyY6RjormpgMk

[@dhaval4570]

@@FreshfromtheFarmFungi I have seen the whole live stream, The problem is my print is on aluminum foil and I don't wanna use the whole print, any workaround that?

[@dhaval4570]

@@18Hands yupp

[@Foxxxxx96]

“Yeah Science....” Jesse Pinkman

[@Memeaux2]

What setup are you using to draw liquid from your media bottle?

[@FreshfromtheFarmFungi]

it’s a media extractor bottle from “lions mane equipment and supply” in Denver

[@adrihalut]

360P max resolution...

[@FreshfromtheFarmFungi]

retro from the start - thanks for the algorithm boost! 🍄❤️

[@LarsLarsen77]

Don't talk while you make LC. You're spitting all over it.

[@FreshfromtheFarmFungi]

lol I usually wear a mask and do my culture work in silence but I have to talk to make them in one take for these - it’s more about the process and ideas then rate of success this is not my main production