Lineweaver Burk plot

A typical curve of enzyme kinetics is a plot of a plot of velocity of reaction vs substrate concentration. As the substrate concentration increases the velocity of the reaction increases until it reaches a plateau, which is known as maximum reaction velocity or Vmax. The drawback of this graph is that the plateau region is not perfectly horizontal. Beacuse of this there is a error in the estimation of Vmax and Km. Hence the researchers started modifying the graphs to get better estimate of Vmax and Km. One of such graph is lineweaver burk plot.

[@rachael4775]

I remember watching this in university and I never forgot how helpful it was. Now 4 years later, I'm coming back to it in medical school

[@niatai9651]

Same!. Except for Pharmacy school

[@Lulu-uw1mj]

the video was posted 2 years ago...

[@rakshitasingh6574]

@@Lulu-uw1mj 😅

[@olaoluwaoyeniyi9948]

​@@Lulu-uw1mjsarcasm bro

[@blueflash3641]

hey what was your bsc in ?

[@theinkedaaila]

This is perfect to understand whatever the hell is this lineweaver burk plot...... just wow

[@Woogie9593]

*watching video after getting all lineweaver burke questions wrong* "hence, lineweaver burke plot is one of the easiest graphs to understand enzyme inhibition"

[@alonzojonathan2553]

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[@dustinameer4343]

@Alonzo Jonathan instablaster =)

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[@alonzojonathan2553]

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[@dustinameer4343]

@Alonzo Jonathan you are welcome xD

[@speci4list326]

I was procrastinating because this seemed really hard to understand. Thank you!

[@bobmogoboya1477]

Yoh!! im writting tomorrow and i thought i was gonna fail this burk plot and now i get it. Thank you!!!!

[@surbhisharma704]

Thank you so much for making it that simple for understanding in a better way

[@stellawanjiku4362]

Thanks, this made it so much easier to understand

[@jyotsnaraut77]

I thought it's very hard But seeing this video clear all the doubts thank you ❤️

[@Drift1]

Thank you! the mathematics explanation helped explained how Vmax and Km value changes can be infered from the Line - weever. I guess thats the point of it, make it simple to see changes.

[@MrCred_]

I thought this was hard u made it so easy thank u

[@itzelhidalgo874]

Fantastic video!! Thank you so much 😉

[@heybriaannaa]

great video! thanks for sharing!

[@katejbradshaw]

THIS video makes it all clear! THANK YOU!

[@ayaahmedmahersayedabdelaal242]

This is so good. thank you!

[@mckenseymiddleton9166]

that helped me a lot, thank you!

[@nicolevillalon9737]

Quick and excellent explanation. Thank you!

[@elijahperry3454]

Lifesaver...thanks a bunch!

[@kamariweaver1536]

What values on the plot are Vmax and what value is Km?

[@logitfau252]

thank u alot :) really helped me for my assignment :)

[@malithaniman1730]

Helped a lot thanks

[@gayatri5996]

Sometimes the easiest math seem difficult @ 2 o clock at night. Thanks for making it clear!

[@mayryelll3822]

the only video that i understood thanks alot 😭🙏

[@AA-kr1ci]

Explained to perfection !

[@peerzadaabrar1800]

Very very Thankful to you. Jazakallahu Khair🤲.

[@sanskritimishra7299]

This would help in my presentation ! ✌️

[@jen-hungfang5437]

awesome channel!

[@nyikomhlarhimusic]

Awesome explanation. Thank you veeeery much 🙏🏿

[@mayryelll3822]

god this was soo easy thank you sm

[@tylerdurden7903]

Great video

[@tukurummulkhairi9794]

Thanks a million times

[@preethimurugesh6722]

Simply superb

[@gatwiringera7567]

thank you very much. Understood!!!!!

[@asiaaaa]

awesome, thank you!!

[@oumaimaboughazala5706]

Thank you so much ,I understand it

[@harshithjagadeeswaran527]

Was really helpful.thanks 😊

[@linam4848]

Great stuff

[@MzPeregrine]

You are probably going save my exams today

[@easyochem]

This is a certified hood classic

[@alyssatipler6980]

I dont understand how you get the -1/km as the x intercept

[@gfkenya9926]

see below from her post. Best to you. "Nancy Barajas 1 month ago (edited) At the x-intercept, the y-intercept is equal to zero. Using the lineweaver burk equation, you set 1/Vo equal to zero and solve for 1/[S] (x-int). This will give you -1/Km"

[@rosekendi8590]

Thankyou easy to understand

[@jokebotter-vanelburg8590]

where does -1/Km come from?

[@nancybarajas4249]

At the x-intercept, the y-intercept is equal to zero. Using the lineweaver burk equation, you set 1/Vo equal to zero and solve for 1/[S] (x-int). This will give you -1/Km

[@JANVI_743]

@@nancybarajas4249 thank you, it was useful

[@prakritipurushottam8427]

You got 1 subscriber today

[@Moye_moye03]

Thanks🙏

[@myra9250]

ThankYou.

[@emilyi771]

Ty

[@abhishekrana5933]

Thank you from 🇮🇳

[@munivet6685]

Excellent

[@user-bq5uw4uy1w]

so helpful! I’m an italian med student

[@abdullahshah7644]

outstanding

[@siddheshpathak9181]

But on X axis why do we take - 1/Km ?

[@krutarthshukla3067]

keeps bugging the hell out of me

[@paritiyasingh5342]

Since y=0, MX=-c. Then x= -c/m. Substitute values to get. X=-1/Km. Because Vmax get cancelled

[@Obamnaz]

@@paritiyasingh5342 Tanksss

[@abhi9973]

@@paritiyasingh5342 nice.thx

[@ahmedshah6277]

You are not telling about what is meant by these values and how it changes with substrate concentration so its very hard for me to understsnd

[@sebastianlerette9450]

🙏

[@vaishuu04]

Thank you!! It really helped!!!

[@anchalyadav3061]

Thanku

[@user-zj7rz5oi9b]

Plz -1/km From where coming???

[@tarunkumarpalli7580]

How -1/km is come on negative side of x axis

[@prismaticseal2553]

I understand the how, not the why or what are any values

[@alhaeri1]

Legend

[@richardshane456]

Reverse hardware engineering use similar mythology to reverse engineer to find the answer incrementally by best guest trial and error feedback logic a natural humanistic approach to solving a problem with the Mathematical mythology of trial and substitute to understand the trajectory spin of any reaction mathematically incrementally with finer granularity of the understanding of any process... A common mistake with observation by maintaining a relative frame of data points that in reality are trajectory spin... Although the graph inferring to the deduc enzyme reaction metric, belaboring the point of graphical representation for a finite metric that does not disclose the granular processes of the line depicted as the rate of change of velocity. It would be interesting to observe the elementary particle processes of a enzymatic reaction... Ty...very interesting Good day

[@AmberU]

Just maybe I’ll make it through biochemistry

[@confucheese]

Weemax

[@BioScienceClass]

More Videos on Lineweaver Burk plot: kzbin.info/www/bejne/rXK6inx9ibeLh6c

[@arnepetric7333]

sef razjasnu

[@justicedauda6117]

❤😊😂

[@MuhammadAli-fj8qh]

1:51 I hate y=mx+c

[@Blaskhartor]

Are you a man or a woman??

[@skellingtonmeteoryballoon]

IF we invert the equation. Can vmax and km should be inverted to plot in the graph. Graph calculator is more than a convenience 🧮