Mushroom Mycelium Tissue Culturing in Liquid and Agar Media | Southwest Mushrooms

Here is our lab process for mycelium tissue culturing for both liquid and agar media. The most important part is cleanliness and a sterile environment to reduce risk of contamination. We do this every 2-4 weeks to ensure a healthy catalog of fast-growing mycelium strains at our farm.
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▶ Cultivating fresh gourmet mushrooms at our urban grow farm. Mushrooms with amazing flavor and full of beneficial nutrients. Our passion is to offer every customer the freshest product in the market. Owner "Mushroom Mike" enjoys sharing the science of mycology while serving top chefs, everyday cooks, and those who enjoy a healthy lifestyle with premium mushrooms.

[@michaelgonzales2057]

Hes just so dense with knowledge I could listen to this guy talk all day

[@FrankDeLalla]

Yes!

[@Paintingtube]

How long does all mushroom liquid cultures Syringes last in room temperatures and Refrigerator? Is growing dove Oyster mushrooms the same process as growing Chicken of the woods? Bye using rye grain and straw hay!

[@visit402]

Yeah this guy is real dense

[@myecofuel]

Yes me too, I listen until I get to sleep...like music to my ears

[@patswayze7359]

It's Basic mushroom growing knowledge. Just say he's dreamy...

[@propositionjoe6936]

It is rather relaxing to hear you talk and there is a lot of useful information. You Sir are truly the Bob Ross of mushroom growing!

[@DaveCharbonneau1]

Happy little mycelium clouds.

[@oldchild527]

Hahaha you both are cool dudes

[@tristanbradford99]

you have the most savage flow hood setup man

[@chuckisnutz]

I think it's 3'x4' panels. Jesus

[@mycosauce7018]

Once upon a time... there was a perfect world... In which I had this set up haha

[@KA0S3FX]

1000%

[@agravy7657]

He has a fantastic setup... but title of most savage probably belongs to someone else 🤣

[@wylesben91]

Flow so good even the mushrooms knew what's up.

[@duftendeskraut6148]

You can really tell that he is coming from the ground, building this up. All the og tips that we get from him, if we don’t have the proper equipment are very helpfull. Today i just started my mykology class in university and i‘m so excited to do all the experiments like i see in you’re videos. Couple months ago i saw a video from you and that got me hooked up to mushrooms, so thanks a lot!

[@recyclingnik]

what do you learn in mycology class in university, are you studying biology ?

[@SirSwanky-zd6el]

Fungi!!

[@NessieTheCatt]

nice have you learned to spell mycology since?!

[@rustymansuetti2234]

Happy shrooming! Great hobby!

[@Lalovision3D]

@@NessieTheCatt 😂

[@luxuriousfir]

Mushrooms will help us traverse the universe not only mentally but physically. Such a great overall nutritional source.

[@johngrady1755]

His setup explains why I get anxiety every time I'm about to inoculate. You either have a NASA setup or live in mortal terror of the mold menace.

[@superbarnie]

What are you working with? Flowhoods are great, but it really isn't strictly necessary for small scale growing.

[@NessieTheCatt]

@@superbarnie prob a shotgun chamber if he’s getting mold issues

[@NessieTheCatt]

Yep that sounds about how my first batch went. Mold mold Mold

[@SirCheese710]

There are definitely in between options... i got a complete HEPA laminar setup and coupled it with a 2x4 foot shelf to make a big work space... i was willing to spend a bit more, but under 1000 dollars for the shelving unit and the complete fan filter and hood setup. If you want to find a cheap HEPA filter go check out Staples and you could easily get a 9x9inch filter for about 30 bux that you could use to make your own clean air box. Question is just how big of a space you want to work in and how much you want to spend. I was lost in my early research but realized there are options for every price point

[@superbarnie]

@@SirCheese710 I wouldn't recommend using those HEPA air purifiers found in stores as a flowhood, generally their filters are not thick enough to create laminar air flow. IIRC, you need the filter element to be at least 6 inches deep for laminar air flow. I'd suggest going the DIY route if you are low on funds and a flowhood is really necessary ( it usually isn't). A 2x4 foot flowhood can be made for around $600

[@gazzabethyname]

Giving out game like there is no tomorrow. Respect.

[@davidcervantes4867]

I absolutely love that when you say something is fun you show no indication that you're having fun haha

[@anthonycoore6562]

I can tell you love this and you’re spreading the love ! Learning tons. Been looking for a way to grow my own food and came upon your channel and it’s opening my mind to even more possibilities. Thanks!

[@DavidRodriguez-ci6dm]

literally can't blink while you speak following your teaching, and its amazing.insane process. I've watched this couple times already but i'm totally new to this topic. Thanks for the time to teach.

[@mikilehto9833]

Great video. I especially enjoyed the part at the end, when he's just talking about his favourite mushrooms to grow and substrates and what not while still doing transfers. Gives me confidence that maybe I can succeed in it too.

[@shemikawilliams4274]

i love how you love what you do! the passion you have just oozes thru. thank you so much for sharing your love!💖

[@A_Train]

My man! You are such an inspiration. "Started from the bottom, now we're here." All success to you.

[@CyberspoonMusic]

Great video man - 'I am a vector of contamination' - best line ever....

[@ericamelchiore4910]

Thank you so much for the video! Awesome video quality and excellent information explained to the level where the hobby mycologist can take it all in and not feel overwhelmed! Can't wait to see more!!

[@hydrogreen1111]

The most educational and informative instructional video on raising mushrooms I have watched. Thank you. Really like your sincerity. Very gratifying.

[@chbitt]

THANK YOU SO MUCH!!! Super fun video. I very much appreciate getting into the little details. Very interesting.

[@BenlshTracker]

Your videos have been invaluable to my university project looking into making mycelium based toys, thank you!

[@matte4123]

the cleanliness in his grow is something to behold

[@victorribeiro8147]

Thank you so much for sharing Mike, the details make all the difference and help to avoid numerous mistakes. There are few people with the willingness and ability to share this knowledge so well and so simply. Again, thank you very much. Hugs from Brazil.

[@joesmith1922]

Likely one of the best mushroom videos out there. Thanks!

[@NickolasHinderer]

Sick set-up, thanks for sharing, keep up the great work!

[@mahoneytechnologies657]

On watching the mixing process I think random alternating spin direction would give a better breakup and mixing. Great to see the processing involved! Thanks

[@Imhotep-Still-Juicy]

“And in this next room, we have the Gamma-Ray Gun where we turn Humans into Mushrooms”. Great videos, very impressive facility, loads of useful info. Cheers

[@ritahkays7410]

Really, 🤔

[@CMDRSweeper]

@@ritahkays7410 Technically he is correct, you can turn humans into mushrooms using gamma rays... But it is not a nice thing :D death by radiation sickness, then you use the body as food for fungi...

[@mohammedalali3380]

@@CMDRSweeper شببييثصجحج١2٢١

[@StevenScheffe]

This was funnier than it probably should have been

[@Thomass7586]

Step away from the video games. Do it now

[@ericr45]

Thank you for your ultimate videos. Sharing your knowledge with others is a science of the wise.

[@digvijaysinghchauhan9101]

You are doing amazing work sir. You have imparted so much of knowledge and that too free of cost. You are an amzing teacher.

[@carsonelias4594]

I've heard a lot of tripping stories, and they are very exciting,I would love to try magic mushrooms but I can't easily get some, Is there any realiable source I can purchase from??

[@patriaciasmith3499]

I have been looking for a legit seller to buy from.

[@mirabelwatson7863]

Yeah, I got mine from bergwilly1

[@mirabelwatson7863]

On Insta

[@markaxel9799]

I have heard about him. Is he really legit?

[@JamesTaylor-ff4dp]

The Trips I've been having have really helped me a lot,I finally feel in control of my emotions and my future and things that used to be mundane to me now seem incredible and full of nuance on top of that I'm way less driven by my ego and I have alot more empathy as well

[@kylehancock3785]

Phenomenal! Clear, easy to understand info. Thank you!

[@FrankDeLalla]

Thank you for this great video! I happened to be doing this for the first time TODAY!!! (Grain to grain.) What awesome timing. 🙏

[@cantho11]

I love this dude dont hold any knowledge back. Thx bro

[@Zayskibop]

man you designed your whole facility like a mad scientist! the bigger im getting and the more consultation based work I do, the more I wish I could have been apart of building the beautiful farm&facility you have at Southwest.. wicked!

[@mathewyoung1134]

Your videos are amazing my dude.. Rookie mycologist myself. I find alot of great content highly useful in my endeavors. Thank you very much.

[@AdamMulholland]

Amazing quality videos! I have picking up tips and look forward to even more.

[@Zulanderr]

Im unbelievably jealous of your setup

[@Z13ux]

Thank you so much for all these little tips and tricks! Love it

[@mumsymaebear]

You are a good teacher and thanks for sharing the finer points in your videos.

[@Lurch-11]

2 years later but I'm so glad I found this vid/channel. So dayumm descriptive FINALLY!

[@melindammelly]

He does a great job explaining how this all works in terms that I can understand

[@fusion9619]

So much here that I don't understand... But as a teacher, I can recognize another teacher, and I'm watching a great teacher here. Keep it up man, I can sense that you're on a very valuable mission

[@mutableparameter4598]

Amazing setup... Starting out just now .. changing my set-up from Cubes to Gourmet. New sub

[@luchacefox259]

lol sad.

[@momsmushroomsjodyfoster5786]

My love of nature and the forest brought me together with mushrooms. And mushrooms became an obsession after that

[@lucasvallerie5439]

Thanks for making these videos. DENSE with info, and he’s so pleasant to listen to 👍

[@NickManeck]

I love the work you do for the advancement of mycology. I just wanted to make a mention of this.

[@XCLU5V]

i dont even like mushrooms, but i cant stop watching all your steps on how to make them!

[@gwartard]

I don't know how I came across this channel. But I'm so happy I did.

[@CassEOpia]

Relaxing to watch and listen to the technicalities. Interesting and fun to learn such things. Seems like a neat person all around. Chill person to work for and with. I'd look forward to going to work every day in such a place.

[@111Lky]

outstanding demonstration, thank you so much!!!

[@mikeywulff3766]

Massive thank you and big love for sharing such incredible knowledge ! Much appreciated :) !

[@Northcoast4dees]

your teaching skills and knowledge are amazing, thank you!!

[@beeman3008]

really enjoyed this video, informative and chill. Eyeing up my agar cultures now to get ready for liquid culture!

[@TigreDemon]

I really love hearing about these kind of things (even though I'll probably never start a culture)

[@pipersall6761]

Youre a great teacher. Thank you for sharing your knowledge!

[@jacobshocklie2928]

Thanks for all the great videos!!

[@NUTRITIONALDIVERSITY]

love this man thank you !!! great setup!!!

[@dombaker8790]

Another BRILLIANT video.

[@jorgesaracco7491]

loved how in min 2:39 the calendar on his right (our left) marks the month of FEB and this was posted on June

[@DiscoveryWonders]

*Notice how WELL he disinfects everything*

[@rev.jimmywonko9615]

I would follow you with a camera like a zen student. That space is beyond awesome.

[@tarsier419]

Nice vid, thanks. A lot of great info! Looking forward to more!

[@fromdusktilspawn]

Awesome set up! Great Technique!

[@meridethtexastwister]

I've learned so much watching your videos. So interesting. Thanks for sharing your knowledge and experience

[@silverrose7554]

Who ever helps you with camera 🎥 work is the bomb . 👋🏼 five great job ! Say hello some time !

[@AB-kg6rk]

Your operation is incredible.

[@whitefordpipeshandmadebymi7238]

Very informative and easy to listen to and understand! Thanks ! Take care! Peace ✌️ from Welland Ontario Canada 🇨🇦

[@inphinitezero5499]

If he was a teacher i could listento him all day without getting bored

[@benjamingreer1660]

This guy is awesome. Keep spreading those spores!

[@Tony-dx3eo]

Lab jacket is a nice touch Mike 🙌🏻

[@cjhification]

I haven't watched a lot of your content yet, just found you recently. Very interesting content indeed and your stacked plate work was very good, I haven't worked in a micro lab for over 15 years so lovely to see. You may cover this in other videos bit in case you don't, your refering to aseptic technique when you talk about maintaining sterility in a lab, I thought that might help some people who want to research further about it. (For the you, it's not important, as you clearly know what you're doing). Also for others the plates come in the bag, sealed for sterility, if you open carefully it perfect to then store them in again, with a little masking tape to close up. I'd only store them like that, if storing blank plates (Petri dish with set agar) before use. I'd tend to use cages to store inoculated plates, but they lived in climate controlled cabinets. Also interesting fact about agar for those new to it, you heat it up to about 85°C (about 185°F) to liquify it and it will then stay a liquid as it cooos to around 37°C (about 100°F) then it gradually set.

[@nathanielcharles2175]

Impressive. Thank you for this! I've developed a passion for mycolocy and I have goals to start growing and producing mushrooms to help people. Good stuff brother! 🤞🏾

[@thenmgarden6471]

You remind me of one of my friends. Very inspirational as far as trying to be clean im going to need a flow hood one day

[@matthewbrown6688]

Wow, very well articulated and informative!

[@eircification]

Flo hood Jesus. Beautiful video , jealous of your setup , appreciate this video. You're a skilled teacher , mad respect my dude.

[@phoenixjonez]

Peace to the Gods, man. Say brotha, let me first thank you and give you your roses. You are well spoken, intelligent, and thorough with your explanations. I've watched a good amount of your videos and I am beyond elated and impressed. I just recently started my journey in mycology, and yes, am very fascinated with the world of fungi. You have been doing this for a long time and your knowledge shows. From every fiber in me, please continue to work your magic...please continue to study and soak up as much as you can, so that you may further your journey as one of Earth's messengers...please continue to be a good father, and if you haven't been, it's never too late to change...and always give thanks to our ancestry and those who came before you, for without them, none of us would be where we are today. Thank you again, king, and Happy growing 🍄‍🟫🍄💯

[@Frostyd241]

These videos are amazing. So inspiring to a brand new grower.

[@vancegilmore245]

Magic Mushroom Mike's Masterpieces of Mycological Manufacturing Methods!!

[@mattnsim]

Thank you! Just thank you.

[@notd4335]

I love your content so much! Plz keep making more

[@ricksflicks-]

Amazing video. Thanks for sharing your knowledge and calm attitude.

[@SweetLife529]

Really wonderful videos! Thank you for sharing all that you do! 👍

[@TheArchform]

Dude, I wish you had a book or taught classes so I could get the whole process.

[@SouthwestMushrooms]

Hold tight, a lot more videos coming

[@JamesCT153]

@@SouthwestMushrooms can't wait!

[@silverrose7554]

Southwest Mushrooms start a paid website to learn rshrooms 101 102 ect ! You will set the standard . Get a Dagree from southwest mushrooms will be the valued standard .

[@leehuntley189]

@@SouthwestMushrooms do you sometimes leave a stir bar inside your liquid cultures?

[@mastertrey4683]

@@jeremyhinken3365 thanks

[@valentinpopa4249]

Amazing videos mate, keep up the good work!

[@loractorres5050]

Thanks for the tips man!

[@andrewedwardsmusic]

Hey, Im in Phx Az as well! Im glad to know you are here in the valley with me!

[@ruftime]

Thank you!

[@HD-cr8ne]

Such awesome videos. Heaps of info and easy to watch. Thank you.

[@valerieyoung2125]

I love the channel. Please keep the videos coming!

[@vk081064]

Brother each and every video of yours is a gem

[@casualgamer3771]

this setup is incedible

[@MicahMysiuk]

Your lab is beautiful, thanks for sharing.

[@miasnieuwoudt8952]

You are awesome. Thanx for all the tips and help.

[@Theoldindie]

It looks so clean, I dont even want to cough on the screen!! :)

[@anthonymasters07]

Awesome teacher. Thank you soo much.

[@Capucino888]

Waw, you are such a great teacher. I find your tutorial as the more practical. Thank you very much for sharing your knowledge. Keep up with the good work... Michel from Belgium.

[@JamesSailor]

I wonder if he would be willing to be a mentor?

[@f.demascio1857]

I envy your set up. Reminds me of my time at FP.

[@babyrob0t]

Thank you so much 🍄

[@justamanandhisvan]

I already cultivate on a smaller scale but I'm expanding to a full size operation next year. Your videos give me some structure on how to setup my area. Also for someone that might be new to cultivating these videos are spot on and have invaluable information in the process so good job man. 10/10 great videos.

[@kereyc5751]

How is the scaling up of your operation going now? Just curious since I ran across this comment and its a year old ;)

[@gargoylekingGWO]

U truly are one smart King love the content keep shining like u do

[@davek2068]

I don't know much about growing mushrooms, hence watching KZbin videos......I am very impressed by what you're doing! Great videos! A bit intimidating as to all the technical processes you've implemented but looks to be very successful! Thanks for making the videos!

[@karloskardenas2414]

Awesome info Teach! Thank you!🙏🏻

[@Axiomatic75]

Very informative video, thanks a lot!