MASTERING TISSUE CULTURE PLANTS | HOW TO ACCLIMATE PLANTS FROM TISSUE CULTURE

[@Miles_Hoffman]

Thank you, I love learning from others mistakes. Please make more content about how different plants need different care. 😊

[@brookewarra9520]

I'm waiting on my very first TC and I'll be honest, I had no idea what I was getting into. I just happened to look up the term tc+prop tonight and seriously I have been through so many videos & felt so confused and overwhelmed until I got to your content. Very informative and straight forward and detailed info. You're a lifesaver. I can't wait for my new little dude to show up!

[@Parkfolia]

I’m so glad this was helpful!! You got this 💪 it’s intimidating dealing with your first tc plants but once you get the hang of it, it’s a ton of fun watching teeny tiny plants grow into their full size

[@alasdairowers-bradley6421]

Such a helpful vid! How much of a water reservoir do you need in the cup and how often are you watering? Do you open during the first 2 weeks to water? Thanks

[@vmota60]

Please do more videos! I am new to ptc and want to learn from experienced people.

[@buffalo7480]

I haven't seen your content else where, but you've nailed this vid. I'd never heard of that media. It takes a little longer, but I would love to see a comparison of different methods or protocols if you do Tc yourself. Great job! Looking forward to more content

[@Parkfolia]

Thank you so much! I can definitely make some comparison videos, it would be an interesting experiment to see the growth pattern of different protocols

[@skepticalhippopotamom]

thanks for sharing!! tbh i would love to see some content on multiplying plants too if you have some time :) !!

[@Parkfolia]

Of course!! I’m going to try and do some filming while I work on plants in the still air box soon 😄

[@hberror404]

Yaaay more TC content on KZbin!! 🎉🎉🎉🎉

[@vivity01]

Just received my first tissue culture from Etsy and was totally lost. Thank you for your professional, informative video! Looking forward to some of your culture offerings. Well done!

[@Parkfolia]

Thank you very much! I appreciate it and glad it helped you 🥰 happy growing!!

[@vivity01]

The only thing I was not clear on was, when using two plastic cups as a dome, did the bottom cup need drainage holes?

[@Parkfolia]

@@vivity01 nope! I keep a reservoir of water in the bottom with this mix so no drainage holes are necessary

[@davidmaisel8062]

It would be nice to have a video showing the multiplication step on a few species and why different mediums are used.

[@elvisgonzalez3798]

Hey, thanks for the great content. Wondering if neem oil can be used as fungicide when cleaning the plant?

[@TheSkyHive]

Just found your channel and subbed! I have been growing fungi for 27 years and recently started a mushroom farm. I have many industrial size pieces of equipment like my autoclave and flow hood, I have ALWAYS wanted to get into tissue culturing and I think it will be my fall project. The Biocouplers intrigue me. Have you made a video about the pros and cons of using them versus older tried and true techs?

[@realpain84]

Great content, so much to learn for me, thanks for sharing ❤

[@ralfvk.4571]

That's such a cool thing, wow. Thank you very much for this professional Video and information. ❤

[@sunshinecarnivores1919]

This is helpful. Most of the plants I want are carnivorous plants and the cheaper ones are in tissue culture.

[@EverythingPlants]

Great instructional video and is this the substrate you send with the plants when purchased?

[@Parkfolia]

I never used to but I am moving to sending this mix instead! My holy grail used to be coco coir and perlite which is still a great simple substrate to use, but I’ve had much better success with stratum 😃

[@sousafamilygarden]

Question please - how to order the tissue culture kit to try it myself - thanks Great Video thanks so very much

[@prettyingreen]

Subbed!! I hope you do more videos 🙌

[@buckbeaksgarden761]

I hope you do more!

[@GrawRious]

I’m about to buy some banana plants that are tissue cultured and this was so helpful.

[@alfredosantos6669]

Good information. Thank you. Happy 4th of July.

[@poeticplants]

How long should the grow lights be on for during the first 2 weeks? Thanks for the video ❤

[@muddypawswanderlust]

Please helllp! 😭 I messed up! I accidentally added 30gr/L of agar instead of 6gr/L. I used an expensive DKW media and other expensive additives and hormones and I don't wanna waste it! Will this too high concentration of agar affects plant growth?

[@Parkfolia]

🥺 hate to break bad news, but I think so..I imagine the agar will be rock solid from 30g/L so I think it will be difficult for the plant to make good contact with the media. Keep me updated how it goes though, I am curious if the plants will still be able to absorb the nutrients with such a dense media!

[@dredope90]

my 2 TC enjoy the faucet half way running on the plant for 5mins so far they are doing good one is 3weeks and one is 1week who am I just putting out my experience or my water is that clean I guess

[@tulsiramdagur9580]

हेलो दोस्त आप कैसे हैं। आपके द्वारा टीशु कल्चर पर बहुत सुंदर जानकारी देते हैं। आप भी बहुत सुंदर हो आपके टेटु भी बहुत मस्त है

[@dream.fiiend]

50/50 peroxide to water to disinfect?

[@jamess1732]

Do they need bright light?

[@rosewest5168]

Hi Do you put the zip lock bag under a grow light please? Or is it best not too?

[@robertl6711]

Can I use RO water?

[@natachapetite1008]

Is it good to leave them on a heatmat ?

[@Parkfolia]

I prefer without a heat mat, it can get REALLY warm in the sealed environment and speed up potential mold growth or melt the plant from the heat in my experience

[@Weightlosspendotuk]

Hi u ever thought about using ozone to sterilize

[@gettinluckyfishin4514]

@Parkfolia, great info! By the way, what brand and model lights are you using on the racks behind you?

[@Parkfolia]

They’re Barrina lights! I believe the LED T8 version

[@ShawnKeuk]

How about using lechuza pon?

[@geronimomiles312]

What would I do with Aquarium plants , sold as tissue culture , that I want submerged? Supposedly it's routine for them to melt.

[@Parkfolia]

I have a few TC plants in my aquarium, they can be a bit harder to establish, i probably lost 1/4 of my dwarf hairgrass when I planted it out so I’m definitely not an expert on the aquatic side of things! The biggest tip I can give is make sure 100% of the gel is rinsed off before planting them in your tank and give it a dose of fertilizer to start growing roots right away

[@Sergiu.93]

What do you use for CT? ms + naa and bap?

[@MrDirkles]

To avoid having contamination issues when using working with growth media for plants i have found that the following process is essential after 10 years working with GM plants. *wash your hands and lower arms thoroughly with soap ( most important step) * wipe down flow hood with 70% ethanol. * Before you put your containers, bottles, plates etc in the working area wipe them down with 70% ethanol. * spray a little 70% ethanol on your hands before you start. * Have a bottle of 70% ethanol in your working space ( This is kept specifically for flow hood) and decant some into a beaker to sterilize tools whilst working. * Have a flame ( away from the ethanol) so that every tool is dipped in the ethanol and then passed through the flame before the tool is used. * If working with plates , only raise the lid the minimum and never place the lids upside down in the flow hood.

[@Parkfolia]

Great tips thank you!! Have you ever used a 10% bleach mixture instead of ethanol when working?

[@MrDirkles]

@@Parkfolia I only use a bleach Solution for seed sterilisation. To save yourself a lot of headaches doing this stuff I'd recommend that people find a published protocol and follow it. For example, this paper (1985). A Simple and General Method for Transferring Genes into Plants. , 227(4691), 1229-1231. doi:10.1126/science.227.4691.1229 Is the most common plant cell culture transformation method used. You can download the full paper by visiting sci-hub This site allows to download any scientific paper even if it is behind a paywall.

[@max420thc]

If you are working with any hemp, marijuana, cannabis plant this will not work. A virus has been spreading through growers crops called HLVd. Or hops latent viroid disease. Alcohol will not kill it and you will spread it through your utensil's. The only thing that kills it is bleach , I suggest keeping your surface area clean with bleach and your tools with bleach then wipe they areas down after using the bleach with alcohol , your plants will not like the household bleach at 20 percent ,

[@max420thc]

I use a 20 percent bleach, new single edge razor blades for each plant , then replace it. Then after using bleach I clean the surface areas with alcohol after that. The plants will not like the 20 percent household bleach. Not dichlor, but household bleach.

[@shellym79]

Could you use just perlite? A bag of fluval is literaly the same price I paid for my TC plant.

[@Parkfolia]

Yes absolutely! I like adding fluval because it releases nutrients but straight perlite with a small reservoir works great as well

[@svcruz]

I notice your distilled water is PC brand, if your in Canada, where do you buy TC plants to ship into Canada?

[@Parkfolia]

TC 101 group is where I've found a few sellers! I don't really buy them anymore, there's one on Etsy as well who I used when I first started and they were great, I think the name of the shop is Plantsme or Plantsme2 something along the lines of that

[@ericalvarez889]

By using two cups with tape aren't you risking contamination? Or am I overthinking

[@Parkfolia]

Keeping a sterile environment isn’t much of a concern anymore once they’re planted, the idea is to keep high humidity and with the cups taped together it keeps the environment sealed at 100% humidity :)

[@ericalvarez889]

@@Parkfolia thank you for explaining! 😊 sorry totally new to all this.

[@arloaHoekema]

Where do you get those little jars from??

[@Parkfolia]

A lot I've kept from past TC orders or purchased from other local sellers who had unused jars! I have found them on Aliexpress too though and they're kind of expensive on there but if you don't need a lot it works great. I believe ULINE also has a few sized jars with polypropylene lids but I haven't tried those yet

[@rickprater7159]

Wonder if I could add very diluted superthrive?

[@dillonchamberlain]

What for?

[@DEAexotics]

great information

[@Parkfolia]

Glad you liked it! :)

[@IIrandhandleII]

​@Parkfolia is it possible to create a plant from the skin or fruit cells?

[@Sama_09]

Where do you recommend to buy the tissue cultured plants ?

[@somerb33]

I just bought some from Orange Lake Nursery!

[@Sama_09]

nice @@somerb33

[@CHIROTHECA]

0.3-0.5% Peroxide!

[@gardeningonly]

Do you use flow Hood?

[@Parkfolia]

I don’t! I’ll be making a video soon on the still air box that I use ☺️

[@gardeningonly]

@@Parkfolia thank you

[@noprialdi5032]

nice vidio

[@tulsiramdagur9580]

Nice information thenks. 👍❤ Watsapp no.

[@charliemagoo7943]

It seems like all the tc videos I see are generic. Mix this stuff up. Throw a piece of plant in there. 2 weeks later you have 1 million plants in a test tube. Hmmm. Ok in a test tube what is considered a plant? 1 root. 1 cell. ??? How to divide the million plants in the test tube without injuring or turning them into a combobulated mess.etc

[@Parkfolia]

I really Appreciate this, honestly since doing it for a few years I tend to forget what is and isn’t a sort of “common knowledge” and I can absolutely make a video breaking down each step even further and explaining terminology

[@charliemagoo7943]

@@Parkfolia will be waiting...I'm sure it'll be a video and not a short

[@Lovely_planet]

@@charliemagoo7943sheeeeeesh

[@tulsiramdagur9580]

Hello friends how are you. I needed blue berry plant.ms midiya.

[@thawiseninja1559]

​@@Parkfolialink that video here

[@daltonaganos4547]

You are gorgeous!

[@Celticninja011]

Why are all the tissue culture hosts on KZbin always super hot?

[@Vgb_ali_official]

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