How to Tissue Culture Cacti & Succulents

Рет қаралды 19,020

Plants in Jars

Күн бұрын

Пікірлер: 79

@TechplantChannel 4 ай бұрын

the merch is seriously cool

@noteasybeingakid7242 2 ай бұрын

Thanks for the educational KZbin videos.

@Turtletopia Ай бұрын

Right up my alley!!!

@floob247 4 ай бұрын

That shirt is super dope!

@morningstar8187 4 ай бұрын

Amazing video. It’s definitely gonna be useful. I actually managed to find a source for the chemicals and plant hormones needed for TC. Seed banks here in Europe release their catalogs of seeds around fall, early winter, so it’s a project for later in the year. I’m mostly interested in germinating seeds using TC methods. Cactus seeds are super fussy when they’re young and need constant humidity. I get so many losses to algae and fungi.

@alecjosephson7275 Ай бұрын

Damn you!!! I don’t need all these awesome distractions on your channel 😅

@jakevankirk872 4 ай бұрын

Rad merch gonna grab me one of those shirts! Great content, love you're working with cacti now!

@noah295463 2 ай бұрын

Great video . Incredible content. Thank you!

@larrya3989 4 ай бұрын

I've been watching your videos ever since I found your channel and I love your setup and what I'm learning from you ❤

@paulocoelho558 4 ай бұрын

Hey, garden guru! I love learning with you. Haha, you're mildly funny like the areoles. Thanks for the perfect expression to characterize you!😂😂😛😛🪴🪴

@maddscyentist3202 4 ай бұрын

I got that shirt. Will be arriving soon.

@manuelsza7064 Ай бұрын

Very nice informative video. Still not sure whether this is faster than traditional propagation through pups and grafts. But still interesting!

@herbertfrischke7921 4 ай бұрын

Kind of related to TC is a more low tech approach. In Takeaway tec or coke bottle tec you place the cactus seeds in a closed container with soil, preferably sterilised to some degree. The small cacti can handle the high humidity just fine, I've had plenty that shared the soil with algae. For propagating cactus plants grafting is great. All cacti can be grafted to each other. It's great fun and really rewarding. Combining both can yield big cacti in a relatively short time.

@maxritter7868 4 ай бұрын

AreolES, not to be confused with AreolAS, which are also live-giving protrusions but slightly different.

@EddieTheH 4 ай бұрын

Nope. They're just a spelling variation on the same word.

@maxritter7868 4 ай бұрын

@@EddieTheH I think that joke flew a mile over your head. A growth on a cactus and a mammalian breast are markedly different things. Unless you are growing spines out of your nipples I guess.

@EddieTheH 4 ай бұрын

@@maxritter7868 No, the joke doesn't work because the spellings are essentially interchangeable, both apply to both things.

@realpain84 4 ай бұрын

Wow! Usually i'm cloning but now i have to try this out...thanks for sharing

@robertbrindley8948 4 ай бұрын

Sniper Wolf of the plant world, we are all grateful for you and your beautiful information 🥺

@taguato2000 2 ай бұрын

For comercial growers TC is a big thing in the germination of many very small and high cost seeds, such as orchids, maybe you could try to germinate store-bought vainilla seeds or something like that, just for fun

@jchastain789 16 күн бұрын

Love ur vids

@Primitivemycology 4 ай бұрын

The way you draw the seeds up into the pipette, I do the same thing with fungal spores for germination on PDA media. It's wild how similar the two practices are.

@fromiraq100 4 ай бұрын

you are great. do you have protocol for palm tree tissue culture?

@teloria77rainbow Ай бұрын

Quick question; when growing succulent seeds in vitro, should they be put on a germination mat? When germinating seeds the conventional way, a heat mat is a must for succulent seeds. The seeds I’ve tried to start in vitro rarely germinate. Let me know what you recommend. Thanks!!

@plantsinjars Ай бұрын

No, I think that would melt the media. I recently had issues getting echeveria seeds to germinate though, so maybe temp was the issue.

@snoywntr2 4 ай бұрын

Can you buy already prepped the tissue culture media in jars so that you can try this without buying all the ingredients and equipment?

@sunx3829 Ай бұрын

Your MS media includes Myoinositol ?

@AntonioDellElceUK 4 ай бұрын

I'll be waiting for Pseudolithus! But maybe you should try something (somewhat) easier and more interesting like a Welwitschia mirabilis? or maybe a Gasteria Armstrongii variegated :D

@michaelfeeley4351 4 ай бұрын

Great video Laur! Would you ever consider trying tc techniques on more woody plants? It’d be cool to attempt on fruit trees or something

@lacroix3931 2 ай бұрын

You don’t sell plants?? What do you do with all of them! ?

@teloria77rainbow 2 ай бұрын

Hey, for the bleach rinse step, do you reuse your bleach solution for the 3 rinses? Do you need new water for the water rinse step, or can you reuse the water for the rinse? What temp do you bring your medium recipe to, to be sure the agar dissolves? thanks for all your awesome videos!

@plantsinjars 2 ай бұрын

No, I wouldn't reuse the water. I just try to have enough on hand to rinse the plants enough times. And I don't use heat when I make the media most of the time, I just throw the whole media bottle in the pressure cooker / autoclave

@teloria77rainbow 2 ай бұрын

@@plantsinjarsIs it okay to reuse the bleach solution for the 3 rinses, or do you need a fresh bleach solution for each rinse? Thanks for the info on the water! Should the autoclave be under the flow hood, along with the shaker? I’ve gotten contamination and I wasn’t sure if the open air transfer was an issue, even if the jar is sealed with a lid. (Not plastic wrapped though) I’ve googled how to asses contamination because I’m curious if it’s from my explant-succulent, like a fungus or bacteria. It had some tiny black specks on it where it looks like necrosis of the plants epidermal layer. My microbiology class was a while ago, but I know there’s a series of tests you can use to narrow down the issue. I’ve been looking on reasearchgate with not much luck. I’d love a video on how to take the contamination and read the results under a microscope, to see if my plant is sick or it’s my “dirty house air”, lol. I love your videos, thank you so much for sharing all your knowledge on TC!! ❤❤❤ I ❤️🌱 ➡️ 🫙channel!!

@SUPERSUDS Ай бұрын

lithops?

@BeeWellSeeds 4 ай бұрын

great vids! have you ever considered doing in-person workshops at some of the plant expos and/or other events?

@everydaymacrocooking 4 ай бұрын

this is a supportive youtube comment

@HoangHuy-vh2gp 4 ай бұрын

can you tell me about tissue culture anubias nana petite what dosage of medium is needed i am trying many times but all failed, the plant does not sprout and grow with: Ms: 2.4g Kinetine: 0.15g Agar: 4g sugar: 15g look forward to your feedback

@EitanV1 2 ай бұрын

Why are there no auxins in the media? Only a cytokinin (BAP)

@shawnbutcher1153 4 ай бұрын

ooOoooOOoo I’ve been waiting for a cactus video

@LifeWithLeavesGH 4 ай бұрын

I had to get one of the new shirts!! They’re 🔥 🔥 🔥!!

@SubieShaves 4 ай бұрын

New sub awesome work

@raybod1775 4 ай бұрын

Love this channel because it seems a bit crazy and I’ll never do it.

@story_mode 4 ай бұрын

Would you try platycerium ? We are in Asia and there is a huge surge of platycerium trend. Special platycerium selling for over 200K

@footballfan1104 3 ай бұрын

Can a hobbyist do this at home?

@plantsinjars 3 ай бұрын

Definitely. My next video is going to be a home tutorial. I also have some on my channel.

@thechemistaliyahya1638 4 ай бұрын

I need to know how to tc anthoruim

@dmatula811 Ай бұрын

It seems like some of your cleaning processes are similar to the brewing process. Star San is what I used to clean jars and utensils that will got into any beer or wine.

@plantsinjars Ай бұрын

My brother does homebrewing-- there is definitely some overlap!

@NikoMC31 4 ай бұрын

If you don’t sell them then what do you do with the hundreds of plants on those shelves?

@yoteango 4 ай бұрын

she doesn't sell them anymore to us plebs :( lol

@AloeLover 4 ай бұрын

Been waiting for this video!!! 🙂‍↕️

@apr888 4 ай бұрын

How about using a sterilized dilute solution of sodium thiosulfate as a rinse after the bleach bath? That should neutralize the chlorine/stop bleach activity and minimize the number of rinsing needed. So something like bleach bath, then sodium thiosulfate rinse, and then a water rinse.

@Micheal-uc5qb 4 ай бұрын

Wow. THIS... Milestone moment!

@franklinwaynejohnson2161 Ай бұрын

Í love your channel. The link for you containers is erroneous. The product displayed says, Do not autoclave with media, the plastic will distort.

@flamesintheattic Ай бұрын

Nice job. My propagation technique looks like guerrilla fighting by an actual gorilla in comparison.

@lkysvn 4 ай бұрын

You're so beautiful and smart omg. You even love science like I do and plants! I'm in love 😂❤

@cooley18 2 ай бұрын

What made you stop selling plants?

@deniskyd1483 4 ай бұрын

WoW!!! So pretty girl 😊😊😊

@steve-adams 4 ай бұрын

The biotilt is interesting to me, but the entire system is so basic and unpolished as to make me think I should just build it myself (that is, assuming it's pretty expensive to buy from PCT). It also looks like it needs a much better solution for locking in the biocouplers. How do you examine the state of a coupler in the middle of a rack, surrounded by other couplers? Lean into the biotilt and peak into the jar from above? I wonder if early detection of issues would be made more difficult that it should be. Perhaps I'm misunderstanding how it works too, but it looks like you need to take out an entire row of couplers at a time to inspect them individually. In any case, I'm looking forward to your video about it!

@salimmazariboufares3118 4 ай бұрын

Areoles is a French plural of the word Areole and it is pronounced ah-ray-OHL for both singular and plural, the 's' is mute.

@TheExperimenthair 4 ай бұрын

Why did you stop selling plants doe?

@teddyruxpin3811 4 ай бұрын

Thanks for your videos. I'm going to give tissue culture a try for the first time. Just a heads up, "sterilize" is different than "sanitize". Sterilize uses heat, sanitize uses chemicals. So technically you're sanitizing the nodes when you put them in the bleach solution, not sterilizing them. But whatever no big deal.

@barcaisbest10 4 ай бұрын

🔥🔥🔥🔥

@andrewspencer303 Ай бұрын

Why did you stop selling plants?

@RealRabbit1124 2 ай бұрын

If you bid on eBay, you literally have to be right there the last few minutes to be sure someone else doesn’t bid on you. People wait for the last minute.