How to Acclimate Tissue Culture Plants

Let's acclimate some plants today! Sorry for all the millenialcore sketch comedy and ancient memes. I was possessed by the year 2013.
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Supplies used in this video:
• Trays amzn.to/47VKn3Y
• Fluval Stratum amzn.to/3KPPrwI
• Perlite amzn.to/3QRYNvY
• Rooting Hormone amzn.to/3KOhdtG
• Fungicide amzn.to/3sgPJ9q
• LED Lights amzn.to/47HFZFw
• LED Bulbs (if you have a smaller space) amzn.to/3KST4lM
• Fertilizer amzn.to/45l5kDH
The lights I recommended above are different than the ones I use in my laboratory! My lights are T5 grow lights with 4 rows of tubes each and they are totally OVERKILL (they are leftovers from the year I got really into cacti...). I had to modify them to not fry my TC plants.
Timestamps:
2:19 Demo
7:10 The First 8 Weeks
9:16 Tips + Tricks
Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.

[@basii578]

Such a refreshing contrast to all the other tissue culture content. You’re changing the game ❤

[@leafjerky]

i thought this was the only tissue culture content creator

[@Sama_09]

@@leafjerky naah there are a ton just like her and others too

[@plantsinjars]

@@Sama_09 lmfao where

[@Sama_09]

i just love the way you present, missing the memy videos !! but after subscribing to your channel I was inundated with so many similar channels !! @@plantsinjars will share the list.

[@D3is3l]

My life is in your hands, I got my first tissue culture coming in next week. Wish me luck dude

[@MBG_Broker]

Great topic, great humour and a wonderful host. 10 / 10 would watch again.

[@copyjockeynk]

Please continue with the millenialcore sketch comedy and ancient memes. I'm excited to try all of this!

[@pepperbuster]

I'm not ready for tissue culture but your humor is 💯. Absolutely entertaining video! I definitely have to subscribe now.

[@joecaz]

The science procedures and explanations are very clear and interesting. I laughed out loud several times throughout. Your videos have the comfortable feel of the old Bill Nye the Science Guy shows, except that you're way funnier and much better looking.

[@nickviramontes7371]

This was very helpful. Thank you! I bought a bunch of cultures when shopping for plants for my dart frog enclosure, and I didn’t know where to start. I’ll try to master this and then I can move on to growing something more expensive, like a spiritus sancti

[@BeOurBee]

There's some pushback against substrates like Fluval Stratum in the aquarium community. The thinking goes that it doesn't do anything special for aquatic plants, and similar calcined clay media like kitty litter or oil absorbent work just as well and cost less than $10/giant bag. A trip to the auto parts store for some Oil Dri or Tractor Supply Company for Safe T Sorb might make for an interesting head-to-head comparison. It could also obviate the need for perlite. For kitty litter, any non-clump and un-scented natural clay version should work as well as the oil absorbents.

[@mattyplants]

I've watched this video probably 6x over the last 6 months but it's by far my favorite of yours. The flash drive, the dip n dots, everything in here is peak TC comedy and content ❤

[@sparklessparkles728]

African violets are beautiful! They need a glow up! Another fun video!

[@Jeff-so3kj]

Very interesting. I think a tour of what you have cultured before would be fun. I would certainly watch.

[@Louis-kw6yk]

I love your videos, i hope you keep doing those

[@raissarocha]

I'm sooooo happy I found your video! I'm about to purchase my tissue culture plants from the same site and was totally lost as to what my next steps would be! Alright, you convinced me to go ahead and do it! 😂

[@smacleod1973]

Thanks! Just got a Spiritus Sancti and you have helped a lot!

[@moon.lit.plants]

Love Orange Lake! I got my first TC from them and they are awesome! Love all your content too! 🌱

[@BioInsaneTitan42hz]

You’ve really gotten great on video editing. Much improvement. Also my son loves begonias!

[@jaderey1824]

Thank you for doing this content. I ran into your videos a month or so ago and in that time Iv gotten into home mycology, so Iv got 90% of the items to do this already. You have inspired me to give this a whole hearted attempt and start/create a plant tissue culture journey. Thank you. Looking forward to more of your content!

[@Benlovesplants]

Love this channel, glad to see an acclimatization video from you. Even happier it appears my two week old first try is off to a pretty perfect start. Looking forward to getting my own TC setup started with the help from your >$200 video. Thank you!!

[@rodscl]

loved your videos! you are so good with the camera. 10/10!

[@endor8witch]

quality content. no fluff. right down to the stuff

[@user-zq3sx8kr2u]

I am very impressed . Great job!

[@goober-ll1wx]

Funny AF as usual, nice vid!

[@Peregrine1989]

So I am not sure if you are interested, but it would be cool to see a version of this for Aquatic plants. As I mentioned in my last message to you, the Aquarium hobby is VERY big into Tissue culture plants as it provides a guaranteed pest free way to bring plants into your aquarium, compared to the other cheaper methods (Plants grown in ponds and lakes, or other aquariums) which often come with snails, planaria and leaches, AND if grown by a commercial grower dragonfly/mayfly larvae. However we don't actually do anything that could be considered acclimating. Standard procedure (as you can tell from a quick google) is to remove the plant from the Media, rinse off as much media as possible and then plant in the aquarium substrate (either Gravel, sand or aqua soil) or attach to the rock if its a Rheophyte or Lithophytes, of either a filled aquarium, or if you are more patient a dry start tank. The former method is WELL known to lead to slow growth initially, as the plant needs to adapt to both being underwater AND out of media at the same time. (Meanwhile the Drystart is similar to what you do here, but less sterile.) Actually TBH, I am curious if its possible to culture in such a way that its already acclimated to being under water. You have mentioned in other videos using a liquid media instead of the solid media used in most Aquarium culture setups. Although that might decrease growth rate.

[@nh251]

Plant girls are way better than party girls.. I'd marry you!

[@joysofplanting8241]

Just got some TC plants, thank you for making this video so entertaining ❤

[@petarzaikov6882]

Your videos made me realize how important hygiene and sterilization is. I've crafted the same 'still air box' but instead of tissue culture , I sterilize soil in the microwave and do my cuttings in a clean and sterile environment. First time I've tried that , 8/12 of my plants succeeded (didn't rot) and grew roots etc. I've made a huge mistake with removing the lit for a whole day, just to find out most of them died out, but it doesn't really matter, it was just an experiment and it worked! So far it works best with softwood cuttings. Also, keep up the good work , its really nice that you are chasing what you love and I admire that!

[@syzygyxv3359]

i loved the dip and dots bit

[@mygamingchannel8575]

I just bought the stratum from your link. Thanks for the good tips.

[@mahjabeengardencenter129]

Love the content. Everything well explained in a very good way. I am greenhouse grower always want to start my own facility for tissue culture

[@tylerforrest1311]

😂😂😂 you make me laugh so many times in one video it should be illegal. 😂😂😂😂

[@jaqhass]

Bell siphons work just fine and is better for the plants (unless the setup drown them) than coating the plants to stop evaporation.

[@boodiewhole3211]

forbidden dip n dots yesss, ty for being funny and informative

[@chubaka9432]

The best and the most helpful video about adoption tc!! Thank you

[@Erixamarle]

I'm so happy I found this cuz I have plants coming from orange and I wanted to do tissue culture.

[@Robert-cd2ht]

Subscribed to your IG and filling out an application for a date. Thanks for the inspiration I hope to try propagating aquatic plants, strawberry and fruit trees.

[@user-co8vc5nd7l]

Subbed for the laughs 😂

[@craigoswald9083]

Enjoyed the 🌱 video! Very interesting. 👍

[@markmartinez2022]

Dude! Awesome 👌 👏 👍 very informative

[@DiscoStuLikesDiscoMusic]

very interesting channel, will be watching more of your videos.

[@brad7090]

Stardust, mermaid, panda,wedding pie, Grandaddy Purps, Ice Cream Cake, Ice Cream Mac.

[@lukestevenson7475]

Hello, Laur. Thank you so much for this excellent content! It gives me a lot of "food for thought" for an upcoming career change application. I'm new to your channel and tissue culture, in general. I'm curious to know if you have heard of any DIYers successfully growing large timber bamboo from tissue culture and, if so, do you think I could be successful using your affordable plastic storage bin hack that you've detailed on some of your other videos? Thank you and keep up the great work!

[@teojara8488]

im in love!

[@marchm-p9485]

This is PERFECT timing, I literally checked your channel yesterday because I was looking for videos on this as I just received my first TC plants. Also, I would a video on how to properly dispose of TC medium. Like if you want to save the jar, where do you get rid of the medium? I’m assuming the hormones and things are a bit of hazard so it can’t just be thrown away right?

[@mariclaireprimeau5654]

I’m guessing you have to find a hazardous waste disposal location

[@hberror404]

The meme choices were 1000000/10

[@ChadAF_YT]

We are the same. If it says OG, cookies, or ethos, I have to have it.

[@MrDeicide1]

Adorable witch growin rare plants n flowers, makin tonsa money 🥀🧸

[@junio_fabian_san]

I will keep looking but I did not see if you made a video of how to get them to root.

[@junio_fabian_san]

Are you making a rooting video, or explain how you get multiple plants .

[@roddyhughte2186]

i love your content and straightforward explanation. I do have one question...do you need to place your TC on a heating mat? what if I don't have any lights to place them under, where is the best place to place them?

[@user-oe6tk9lq2i]

I would love to start doing this. I have the passion and love for unique and rare plants. My home is a jungle. Did you go to any schooling to learn all of this? Please teach me Sensei.

[@thepalsar2449]

This is such a informational video!!! Wish me luck with my very first attempt acclimate some alocasias 🤞🙈🌿. I'm wandering if we can use deionized water instead of distilled and still have success...

[@badakhshanaryana8943]

Hi, I just came across your channel, super helpful, thank you❤ How can I find out what is causing contamination in my media?

[@user-qk7nu7td6n]

Do ya thang girl👍

[@zoenightshade2332]

loved the video, I always learn so much! One question at 8:26 you mention against removing the humidity dome unless the plant needs terrarium like conditions and show a picture of a Sarracenia (pitcher plant) but that genus is entirely north American based and to my knowledge is recommended for outdoor growing not a terrarium? If it was the unrelated Sun Pitchers from South America Heliamphora then I'd understand because they live in high elevations. Idk I feel like there's a lot of misinformation out there that all carnivores plants are tropical and need terrarium care, and while there's a few genera that do, most like the Venus flytrap (native to N. Carolina) don't

[@MoleManMattG]

love plants

[@brucetutton7897]

Nice tip re the IBA, cheers!

[@bellaterlaje]

I have the same rooting powder xD

[@nito85]

Could you make a video about making keiki paste please? 😊

[@gavincarr4566]

Hi love your channel could u do acclimating in aeroponics thanks

[@deanerrol1026]

Hello I was wondering if there is a way to un stunt the growth? One put out a new leaf, but after that it's been at a stand still for the past couple months. Should I grow it in water instead?

[@Kay42oo]

YAYYY your finger is better! :)

[@dillonchamberlain]

Thank you. I have nepenthes bicalcarata coming in November and have a much better idea how to transfer them

[@marygorchidsmore2058]

Hi Lauren, great video! Can't wait to see those babies grow bigger! Can you please let me know what is the brand you're using for fungicide?

[@plantsinjars]

It's the one! amzn.to/3sgPJ9q You might be able to find it for cheaper in person or on another website though

[@CriticoolHit]

Thank you for the IBA tip.

[@Cfggghgfdsszz]

Pretty and intelligent pretty intelligent

[@wancrit2317]

great video, are you going to do a video for aclimatising plants form the biocoupler? is the process the same?

[@plantsinjars]

The steps would be the same! Taking plants out of the BioCoupler is actually a little easier because you don't have to deal with solid media sticking to the roots

[@stoned_Evee]

This is very interesting, but what do you do with TC?

[@PhillipXJ9]

I have a few questions… I use Professor Monstera Method, do you think it will work for TC? I dont want to burn out my plants with the fertilizers.. But I love how they’re tailored to monstera so i’m hoping i can keep them. Except the medium would be cocoperlite.. I can list the fertilizers if needed also can i use 100% fluval stratum? If i cant use PMM that is..

[@petersebborn306]

I use Maxsea on my Pinguicula plants.

[@josemontalvo4532]

Do you have any Dwarf Hairgrass, and Purple Knight Cultures available for aquarist hobbyists?

[@TheYokus]

I was not expecting this kind of KZbin content but I was so wrong

[@steffd8822]

Hi! Thank you for sharing, I bought a Burle Marx flame TC & nervous about acclimation. Would you be able to use hydrogen peroxide instead of buying an antifungal agent?

[@BrandonWilliams-ng7st]

Hey Laur, what length forceps do you use?

[@35pianoplayer]

can I use vermiculate as a substitute for fluval stratum?

[@PlantCellTechnology]

🎉🎉🎉

[@sunshinecarnivores1919]

Most of the plants I want are carnivorous and the ones in tissue culture. This would probably work for them too.

[@garycoetsee4934]

Question re the Wilt Stop. The active in this is Pinene. From what i have been advised is Pinene in the incorrect dosage can block the plants completely from transpiration. This is potentially also dangerous. Wouldn't a silica based mono-silica acid be a better option to address wilting and other stresses for the tissue culture plants? there are other benefits to the silica in this form i'm just wandering if this isn't a better and safer option as it also protects plants immune system? Your thoughts would be appreciated

[@shellym79]

Is there a reason you can't use just plain perlite or sphagnum moss?

[@saifsawafi2773]

I purchased 12 jars of Musa Florida and Musa Nono tissue culture but all of them died 😔. However, I'm not giving up. This time, will try your method and see how it goes. I think my 1st mistake was using a container that did not maintain any humidity at all. 2ndly I kept on opening the cover every 2 to 3 days for healthy check, and somtimes adding more water to the planting media.

[@nemo9396]

What fungicide and concentration do you use?

[@ninan3311]

Hi. Hoping you’ll answer - I have deflasked some drosera yesterday and was wondering if I can leave them in 100% humidity for 2 months [im travelling in and out of the city at the one month mark, so would rather do the acclimation process after I’m back properly]

[@lion-ty6eu]

Try to use a uvc lamp to disinfect

[@veryberry39]

So I'm one of the dummies who bought a tissue culture from Epcot. It's only around 3 months old right now, so I think I have a bit before I need to remove it? But it's so pretty and it's growing so happily that I'm terrified of taking it out and killing it. 😭 I guess I'll be doing all the research in the next several months, to strengthen my chances!

[@plantsinjars]

Those kits are so cute! I need to look for them next time I go. You could take it out if you wanted :) the steps in the video should work for dragon fruit

[@caviincursu]

does anybody know if it´s safe to throw away the jar with the medium? if it isn´t safe to be touched then could it be harmful for the environment?

[@shellym79]

Are natural fertilizers like espoma more dangerous to TC babies? (It's just that that's what I have on hand.)

[@alfredgomez6064]

Where can I get IBA that you mentioned ?????

[@TheRipdub]

I told Orange Lake Nursery you mentioned them, and I had to message them about an order I received. They said they'd check it out. So if they reach out to you, hook a guy up with a syngonium 😂

[@TheRipdub]

@@plantsinjars Okay! Just did.

[@beeopper]

Also If you are looking for a editor and Graphics artist, I've been looking to continue video editing since finishing university and wouldn't mind showing you or looking to edit for ya

[@valentineartstudio]

I love these videos you make micro culture seem so much more approachable I dont know if you're planning to do another Q and A but can you use these processes on rubus seeds? Specifically rubus chamaemorus as I can only seem to find seeds and not any sort of cuttings and they are known for reproducing asexually

[@MrDirkles]

I'd suggest that you search on Google scholar for a protocol

[@sdqsdq6274]

wait a min , is tissue culture plants that weak ? how that it die easily ? and there lacking of miniature african violet in the market , i cant find any locally

[@mayotoons4310]

How many moths are we in deep at this point? From the beginning of the hex plant till they are ready to acclimate?

[@puonjairo777]

Does this proses works for trees or just plants?

[@merileelifeisbutadream]

Do you use Miracle Grow Perlite? I know you said that you want to feed them later and that they are delicate when it comes to fertilizer burn. I'm just wondering if your babies are truly ok with Miracle Grow?

[@atyoukid]

Cant find the fungicide! Any other recommendations?!

[@bellaterlaje]

What's the fungicide you used?

[@toeknee8111]

What fungicide do you recommend.

[@bun8Cookie]

Idk if anyone will respond but anyways… Instead of the anti fungi solution can I soak the plant in a mix of distilled water and peroxide??

[@thomasvanwyk]

Amazing job well done how are you

[@GeraldBlack1]

I would clone some blue java ice cream bananas!❤

[@blacknass1943]

very intresstin :)